BioXolver

By Xenocs

BioXolver: Accelerate your biostructural research Until now, SAXS measurements on biological samples were limited to synchrotron facilities or low throughput laboratory setups requiring large sample volumes. The BioXolver is designed to overcome these limitations with the following features: - Synchrotron-quality data in your own lab - Optimized and automated configuration for biological materials - Simplified, high-throughput data collection and data analysis workflows - Low volume capability: Work reliably with volumes as small as 5 µL. - Characterize complex samples in near-native conditions High quality SAXS data in the lab Measurements previously only possible at synchrotron facilities can now be performed in the lab. High throughput measurements and results Full automation from sample to final results on up to 192 samples. Low sample volume consumption Down to 5μL. The lowest sample consumption on the market. In-line SEC and UV-Vis combined with SAXS Characterize small and large protein species with real-time concentration measurement. High data quality Measurements once limited to synchrotron facilities can now be performed directly in your laboratory. With the BioXolver you obtain: - Structural insights into proteins and biomolecular systems under near-native conditions. - High quality data for accurate modeling and interpretation. - Precise determination of key structural parameters. Due to weak scattering from biological samples, high quality SAXS measurements require an instrument optimized for maximum X-ray intensity and minimal background noise. The BioXolver combines both capabilities in a single laboratory platform. Such performance is achieved by combining the Genix3D long lasting source with new patented FOX 3D multilayer optics having a very high solid angle. The BioXolver can be installed with different source options. It can integrate a MetalJet source to obtain the highest X-ray beam intensity possible in a laboratory instrument. It can also be installed with the Genix3D, a bright microfocus low maintenance source with intensity levels only previously accessible with high power rotating anode sources. The BioXolver uses state of the art collimation slits that greatly reduce noise and provide a clean beam. High quality detection is achieved with hybrid photon counting (HPC) detectors integrated inside a vacuum-sealed chamber with no interfering window in front of the detector sensor (windowless detector). This ensures that weakly scattering samples can also be measured at low concentrations. Measuring the background level of the instrument is further improved by proprietary automatic cosmic background removal algorithm. Windowless HPC detectors also allow beamstopless measurements. - Captures the direct beam throughout acquisition for accurate transmission measurement for precise absolute intensity normalization. - Enables measurements at lower scattering angles, limited only by the sample. High throughput measurements and results Full automation from sample to final results on up to 192 samples. Streamline your laboratory workflow. With the BioXolver, standard well plates are loaded and measured automatically using the in-line pipetting robot and automated software suite. - Maximize uptime with a simplified “samples-in, results-out” process. - Minimize manual intervention with automated in-line pipetting. - Scale your research with high-throughput support for up to 192 samples. - Ensure data quality through repeatable and reproducible measurements. The BioXolver’s pipetting robot ensures reliable sample handling: - Prevents cross-contamination with disposable tips - No sample loss thanks to tubeless handling - Fully automated cleaning and drying of the measurement cell The BioXolver includes powerful analysis tools for rapid and objective interpretation of biological SAXS data. The patented LipoSAXS algorithm from Xenocs enables model-free characterization of vesicles and liposomes, removing the need for subjective core–shell modelling. Automatically determine key parameters: - Bilayer head-to-head distance - Number of lamellae - d-spacing for multilamellar systems Quickly compare large datasets and monitor structural consistency between samples or batches. Key capabilities include: - Feature clustering analysis - Similarity metrics (Chi-squared, Pearson, CorMap) - Full similarity matrix visualization - Trend tracking across datasets - Reference comparison with PASS / FAIL decisions Both parameter extraction and similarity analysis are performed in seconds, making the software ideal for high-throughput studies, automated workflows, and in-line analysis on the BioXolver. Low sample volume consumption 5μL minimum sample volume requirement – the lowest on the market. High-precision handling is powered by the seamless integration of our in-line pipetting robot, the BioCUBE flow cell, and Machine Vision monitoring. - Reduced operating costs from lower sample consumption. - Maximize research impact with more data points and greater resolution of the biostructural design space. Each sample is first automatically injected into the BioCUBE measurement cell with the tubeless pipetting robot, then positioned into the beam for measurement. - Thermal Stability: Samples are maintained at your selected temperature, from the well plate to the measurement cell. - Integrated Analysis: Optional UV/Vis capabilities for real-time sample monitoring. A digital eye on your science. Using Machine Vision, the BioXolver monitors the sample volume at all times. This provides continuous feedback to the control software, keeping the sample perfectly positioned in the X-ray beam for optimum data quality. In-line SEC and UV-Vis combined with SAXS Characterize small and large protein species with high precision. In-line size-exclusion chromatography (SEC) separates protein species, from small monomers to large complexes, ensuring clean, monodisperse samples. Integrated UV-Vis measures sample concentration exactly at the SAXS exposure point. Together, they provide accurate, reliable data even for polydisperse, heterogeneous, or aggregation-prone systems. Bring synchrotron-level characterization to your lab. Combine the BioXolver with SEC to resolve even the most complex protein structures. - Superior sample purity: Ensure monodisperse samples by separating species in real-time. - Complex protein dynamics: Analyze structurally heterogeneous, aggregation-prone, and multi-domain proteins in dynamic equilibria. - Universal compatibility: Works with any HPLC system, simply connect your column directly to the BioCUBE flow cell. Real-time concentration monitoring for optimal SAXS results. - Precise post-column tracking: Automatically account for sample dilution after the column to ensure accurate concentration data. - In situ measurement: Get real-time data directly in the BioCUBE cell, before, during, and after X-ray exposure. - SEC-SAXS Optimization: Perfect for complex experiments where concentration fluctuates dynamically during elution. - Total sample integrity: Monitor stability throughout the entire run to guarantee the reliability of your structural models. - Microfocus sealed tube: Cu, 30W/30µm*, point focus. (* DIN EN 12543-2) - MetalJet F source (Ga), 250 W - Rotating anode X-ray sources (Cu and Cr/Mo/Co on demand) 1.2 / 3 kW – 70 μm - Motorized Dual or Triple source (Microfocus sealed tube: Cu/Mo/Cr combined with MetalJet or Microfocus rotating anode) Aspheric single reflection 3D beam conditioning multilayer optics Motorized scatterless slits Xenocs thermalized BioCube flow-cell with camera and pump Pipetting robot with disposable tips Up to 2 x 96 well plates (thermalized) Down to 5 μL Automated cleaning and drying with three cleaning fluids Hybrid pixel photon counting detector Dectris Eiger2R 4M or Eiger2R 1M In-vacuum motorized detector for continuous change of sample to detector distance <0.003 Å-1 / MW < 1.5 GDa / Rg < 270 Å 4.2 meters UV-VIS, SEC-SAXS Installation, training, hot-line, maintenance contract