By Cnr Ifn
The "Single molecule sensing group" exploits the know-how in materials science, condensed matter physics, process design and development, biosensor manufacturing, molecular interaction dynamics and the available facilities for micro- and nano-technologies. In the framework of a long-standing collaboration with the Departments of Physics, Chemistry and Farmacy at the University of Bari, IFN "Single Molecule sensing group" research activity is focused on the development and fabrication of immuno-biosensors based on large-area electrolyte-gated field-effect transistors capable of selective and reliable detection of tumor markers, immunoglobulins, DNA, m-RNA, C-reactive protein with a sensitivity down to the single molecule. It is foreseen that the research activity may have a strong impact on key application fields such as point-of-care testing. Fabrication of electrolyte gated transistors for single molecule biosensors: - Substrate: flexible (kapton, PEN), or rigid (silicon wafer, glass) - Interdigitated source and drain gold electrode: UV-Litho- & E-Beam Lithography fingers - Sensing gate: flexible or rigid substrate, bio-modified with ̴ 10 12 bioreceptor/cm 2
