Provides an extensive analysis of T cell subsets in any given tissue Covers T cells and NK cells including comprehensive phenotyping Markers: CD45, CD3, CD4, CD8, CD25*, CD44*, CD49b/CD335, CD62L*, FoxP3*, IFNγ*, PD1*, MHC multimer*, viability dye. (* = changeable markers). Includes flexibility by having seven changeable markers to decipher your specific focus. Examples of focus could include - Evaluation of T cell exhaustion using PD-1, CTLA-4, TIM3, KLRG1 - Evaluation of proliferation using Ki-67 - Evaluation of adoptively transferred cells using CellTrace or CD45.1/2 - Evaluation of cytotoxicity using CD107a (degranulation), IFNγ, perforin and granzyme B - Evaluation of cytokine production including IL-2, IL-4, IL-9, IL-10, IL-17, TNF-α, TGF-β - Evaluation of effector/memory phenotypes using CD44 and CD62L. CD8+ memory Stem T cells (T SCM) can be included using a combination of CD122, BCL-2 and Sca-1
