Quantification was performed using the AI LNP Profiling analysis workflow on ONI’s CODI platform. This analysis consists of four steps: drift correction, SMLM filtering for individual channels, machine-learning-based clustering applied to the PanLNP localizations, and a localization-based ligand positivity tool and an AI-based counting and abundance tool for cargo. Positivity for the ligand channel was calculated with a minimum of 3 localizations (min locs), as previously described in the LNP Profiler user guide. Note that some optimization of the imaging and analysis settings is required. Contact ONI for assistance.
