Plasma treatment is used in electron microscopy, including cryo-EM, to clean and modify sample surfaces, enhancing imaging accuracy. By removing organic contaminants and creating a hydrophilic surface, plasma treatment improves the adhesion of samples and reduces charging effects during imaging. In cryo-EM, it aids in the uniform distribution of vitrified ice, ensuring better sample preservation and image quality. Plasma-treated grids enhance sample adherence and reduce beam-induced movement, crucial for obtaining high-resolution images of biological specimens at cryogenic temperatures. Overall, plasma treatment is essential for preparing pristine, stable samples for electron microscopy.
