Provides an extensive overview myeloid and selected lymphoid subsets in any given tissue Covers several key populations and activation marker expression in some of these subsets - Monocytic MDSCs (Mo-MDSCs)/classical monocytes, - Non-classical monocytes - Polymorphnuclear MDSCs (PMN-MDSCs)/neutrophils, - Eosinophils - Plasmacytoid dendritic cells - Classical dendritic cell 1 and 2 including migrating subset (CD103+) - Tumor-associated macrophages/macrophages Markers: CD45, CD11b, CD11c, CD19, CD64, CD68, CD103*, CD86*, CD206*, CX3CR1, Ly6c, Ly6g, Siglec-F, Siglec-H, MHC II, XCR1, viability dye. (* = changeable markers). Includes flexibility by having two changeable markers to decipher your specific focus. Examples of focus could include - Evaluation of enhancement of antigen presentation using e.g. CD80, CD86, IL-12, MHC II and MHC I bound epitopes (in a few models) - Evaluation of suppressive markers such as IL-10 and PD-L1 - Evaluation of macrophage polarization markers such as CD80, CD86, CD163, CD206 and PD-L1
