By BioNavis Ltd
In the traditional multi-cycle titration method (figure B), analyte samples are injected over the ligand surface at different concentrations. After sample association, the analyte is allowed to dissociate from the surface by washing with buffer. Before injecting the next analyte concentration, any remaining bound analyte is removed by injecting a suitable regeneration solution. KineticTitration (figure A) eliminates the need for regeneration between sample concentrations and significantly reduces the time required to run a full biomolecular interaction experiment, potentially by up to half the time depending on assay type and number of concentrations. In a typical KineticTitration procedure, analyte samples are injected over the surface in a series from low to high concentration without dissociation and regeneration steps between the sample injections. The dissociation rate is measured after the last analyte sample.
