Consensus Target Design for viruses, bacteria, fungi, and parasites. Providing completely automated design of a PCR assay with unprecedented coverage, sensitivity and specificity across variations of a target. Design a simple assay to detect and differentiate between closely related genomes. Consensus ™-has been rigorously validated for the detection of thousands of viruses, bacteria and human targets. It is useful for applications such as: infectious disease variants for bacteria, viruses, human genomic targets, and mRNA profiling. It also provides consensus design for single panels (i.e. detecting all variants of pandemic 2009 influenza A virus or one set of “universal” primers for all 10,000 FluA variants). Consensus™solves these problems by addressing the following: Best-in-class Design Algorithms - The novel assay design algorithm behind Consensus™ is built on a set of empirically-derived thermodynamic parameters that calculate oligo hybridizations from first principles (rather than derivative complementarity rules). - A wide variety of potential PCR artifacts are accounted for in Consensus™ such as: false negatives caused by target secondary structure, undesired primer-primer interactions, false primer-amplicon interactions, false amplicons to background genomes, and low coverage due to sequence variations. - Consensus™ can handle large playlists such as the entire human genome, human RefSeq, human microbiome, bacterial diversity panel, soil microbes, or all known viruses, with many pre-imported sequences provided in the software for common use. Fewer Laboratory Development Cycles - Consensus™ designs tend to work out of the box, reducing the number of lab iterations needed to arrive at a viable assay. This saves time (3-8 months on average) and associated costs, and gets your assay out faster. Scalable Designs - Custom platform, laboratory, and business requirements (e.g. multiplexing) can be accommodated by Consensus™ to optimize designs for use in high volume commercial laboratories, reducing costs of running your assay at scale. Consensus™ regularly saves organizations 6+ months in work by using inclusivity and exclusivity panels for diagnostic quality assays.
