Next Generation High-Throughput Screening LifeSensors’ diubiquitin substrates represent a new class of substrates for the continuous fluorescent measurement of true isopeptidase activity. The IQF assay is expensive to run, requiring multiple different variations of IQF in order to properly screen for linkage-specific DUB activity. The UbiProbe platform is designed to be a less-expensive, more complete platform for easier DUB screening avoiding the specific assay requirements of IQF screening, while also addressing manufacturing and background concerns sometimes associated with the IQF assay. Not only limited to linkage 48 or linkage 63 screening, the UbiProbe assay can also be used for K6, K11, K27, and K29 screening allowing a reliable assay for studying DUB cleavage in a consistent methodology. Service Highlights * Superior to Ub-AMC and CHOP Assays * Greater Dynamic Range * Amenable to high-throughput screening (HTS) and miniaturization * Less compound interference * Less Signal-to-background
