Amine Sensors SEN-AU-100-10-AMINE Regular price $329.00 USD Sale price $0.00 USD Amine Sensors are used to covalently immobilize biomolecules containing free carboxyl groups onto the sensor surface for analysis in OpenSPR instruments. Note that to couple the biomolecule to the sensor surface via EDC/NHS chemistry, the EDC/NHS activation and blocking takes place on the biomolecule. This method is typically only recommended for biomolecules containing a COOH tag for coupling. Sensors are provided in a package of 10 or 3. It is recommended to use with Nicoya's Carboxyl Optimization Kit, which provides the coupling reagents (ie EDC/NHS, etc.) required for surface preparation and sample immobilization. Additional information Quantity 10, 3 Storage Temperature 4-8°C Shelf Life 6 months Ships Within 1-2 weeks
Meet Revo, the four- or eight-channel digital SPR benchtop system that’s high impact—not high cost. Revo fills critical gaps in SPR by combining digital microfluidics with nanotechnology-based biosensors in a compact, affordable system. Its disposable cartridge supports the same throughput as a 96 well plate (four-channel), without fluidics maintenance and with crude sample compatibility. With low sample requirements, full automation, and consistent, publication-quality data, Revo makes gold-standard SPR accessible, scalable, and cost-effective for any lab. Digital SPR, simplified Revo brings the precision of traditional SPR into an accessible, intuitive benchtop platform. By merging digital microfluidics with nanotechnology-based biosensors, scientists gain direct access to high-quality, reproducible kinetic data, without the training, maintenance, or complexity associated with legacy SPR systems. Low sample, high impact With just 2 μL of sample and up to 18 interactions per four-channel disposable cartridge or 36 interactions per eight-channel disposable cartridge, Revo dramatically reduces sample consumption and hands-on time. Automated serial dilutions and buffer movements minimize workflow burden, enabling faster iteration and more cost-effective experiments across research teams. Maintenance-free reliability Revo’s disposable cartridges eliminate fluidics maintenance and support interactions even from crude samples. This ensures consistent performance, minimizes downtime, and empowers users to generate publication-quality data again and again, without specialized skills or costly service contracts. Supported Applications Binding Kinetics & Affinity Measure real-time association and dissociation rates to understand how molecules interact, how strongly, and how fast. Revo provides high-quality SPR kinetic data ideal for biologics engineering, peptide discovery, antibody optimization, and early mechanism validation. Hit Screening & Ranking Quickly identify the most promising candidates with low sample consumption and automated workflows. Revo’s cartridge-based system allows screening of up to 36 interactions per run, even when working with crude or precious samples. Quantitation & Titering Quickly measure the concentration of your proteins in crude expression media. Automated sample handling, and integrated concentration determination improve accuracy while minimizing hands-on time. Perfect for QC, translational teams, and academic labs. Custom Protocols Powered by digital microfluidics, Revo supports flexible assay design, enabling unique workflows, and tailored experimental configurations that are not possible on traditional systems. Digital microfluidics meets SPR Revo integrates digital microfluidics with advanced nanotechnology-based biosensors inside a sealed, disposable cartridge. This architecture eliminates fluidics maintenance, reduces sample use, supports crude samples, and ensures consistent assay performance, bringing gold-standard SPR data to any benchtop. Product Specifications Throughput: 4 channels; up to 18 interactions / 90 curves per assay 8 channels; up to 36 interactions / 180 curves per assay Sample Volume: Minimum 2 μL Cartridge Types: Multiple surface chemistries; crude-compatible Technology: Digital microfluidics + LSPR biosensors Automation: Automation compatible with software API; Automated sample/buffer movement, serial dilutions Maintenance: None (disposable cartridge) Footprint: Benchtop system – 1.5H x 1W x 1.5D (ft) Affinity Range: pM – mM Applications: Kinetics, affinity, screening, quantitation, custom protocols
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Alto integrates digital microfluidics and nanosensors, making it the world’s first digital SPR instrument. Alto redefines SPR, with 16 channels of innovative DMF and nanosensors in one disposable cartridge that contains your entire experiment. The result: a technology that offers the ease of use of BLI and the high-quality data of traditional SPR. Alto’s integrated cartridge combines 16 parallel sensors with all of the samples, reagents and buffers needed for an SPR assay. Automated serial dilutions of each sample are performed on the cartridge, allowing for measurement of up to 240 total binding curves in one experiment—all without user intervention. Once the assay is complete, cleaning up is as simple as removing the cartridge. Versatility Comes Standard: With a wide range of supported applications and sample types, Alto is designed to meet your SPR needs. Assay Modules: Kinetics/Affinity Characterization (single-cycle, multi-cycle); Kinetics/Affinity Screening; Epitope Binning/Mapping; Quantitation; Custom Protocols. Sample Compatibility: Serum; Cell lysate; Cell culture supernatant; Crude samples. Molecule Types: Proteins; Antibodies; Peptides; Oligonucleotides; Virus, VLP, exosomes; Carbohydrates; AAVs. Reliable, high-quality data: Alto is trusted by scientists everywhere to deliver consistent, publication-quality kinetics data that’s reproducible across instruments, cartridges, and users—again and again. Applications - Basic Research: Mechanism of signaling, action analyses. Alto enables high-throughput binding kinetics and affinity measurements from ultra-low volumes, empowering interaction characterization studies with precision and ease. Biopharmaceutical Drug Discovery: Protein, antibody, peptide, and oligo candidates. Alto simplifies the lead identification process with high-throughput screening, reliable real-time kinetics, microsample usage, wide dynamic ranges, and automation. Hybridoma library screening and quantitation; Antibody-antigen, Antibody-Fc receptor kinetics; Developability testing; Epitope binning. Drug Development: Alto streamlines therapeutic development with high-throughput, automated binding kinetics and affinity from antibodies to peptides, supporting epitope binning and biosimilar testing while enabling regulatory-ready data capture and minimal sample consumption. Antibody Titer; Cross-species reactivity assays. Vaccine R&D: Alto simplifies testing and saves resources, adapting to your testing needs to provide unmatched power, while delivering rapid, high-quality results without compromise. Antigen characterization; Antibody binding assays in serum; Virus-like particle characterization. Applied Research: Alto empowers applied research with rapid, low-volume binding kinetics and affinity measurements. Ideal for reagent validation and assay development, this instrument can deliver reliable, label-free data from crude or purified samples. Reagent characterization (quality assessment). Digital microfluidics: the future of SPR. Digital microfluidics (DMF) is a liquid handling technology for manipulating nanoliter-sized droplets across an array of electrodes. Contained within the Alto Cartridge, DMF brings the samples and buffers to the SPR sensor without the need for the pumps, tubing, or flow cells used by traditional label-free systems. This means Alto is compatible with crude samples (like serum, lysate, and cell culture supernatant), while eliminating the need for maintenance—saving you time, money, and resources. Beyond dramatically increasing ease of use, DMF cartridge design breaks traditional SPR limitations by: Decoupling interaction time from sample volume: With its DMF-powered droplet oscillation, Alto Digital SPR is designed to enable unlimited sample interaction time without any sample dispersion or evaporation. This allows users to optimize ligand-analyte interactions without being limited by flow rate or sample volume. Improving assay flexibility and scalability: Division of fluid into independently programmable units for manipulation results in a system that can be reorganized to provide new functionality on demand (Custom Protocols) or scaled based on experimental requirements. Improving precision and accuracy: DMF-based cartridges that automate sample manipulation allow Alto users to reduce hands-on and sample preparation times by up to 70%, which cuts user-to-user variation, and improves reproducibility. Product Specifications: Throughput: 16 channels; up to 72 interactions / 360 binding curves. Sample Volume: Minimum 2 μL. Cartridge Types: Multiple surface chemistries; crude-compatible. Technology: Digital microfluidics + LSPR biosensors. Automation: Automation compatible with software API; Automated sample/buffer movement, serial dilutions, robotics compatible. Maintenance: Minimal. Footprint: Benchtop system – 1H x 1W x 1.5D (ft). Affinity Range: pM – mM. Applications: Kinetics, affinity, screening, quantitation, epitope binning/mapping, custom protocols.
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Advanced protein stability, made simple Plate-based intrinsic DSF (differential scanning fluorimetry) delivers clearer stability insights faster, more affordably, and directly in your standard microplate workflow. SUPR-DSF is a benchtop microplate reader that measures protein stability using intrinsic fluorescence – no dyes, no proprietary consumables. From formulation and manufacturing to automation, it delivers reliable melting data with minimal sample prep, helping scientists move faster with confidence and clarity. Go beyond melting temperature Tm tells you when proteins unfold. Tagg shows when aggregation begins. Neither tells you if proteins refold. With MSF, SUPR-DSF measures both melting temperature (Tm) and non-reversibility onset (Tnr), revealing when structural changes become irreversible, often before aggregation is detectable. Designed for cost-efficient screening Run up to 384 samples in a single experiment using standard, low-cost qPCR plates and seals—no proprietary consumables required. With low 10μL sample volumes and a broad concentration range (from <0.1 mg/mL to 100 mg/mL), SUPR-DSF offers the flexibility to handle diverse protein samples while maintaining seamless integration into existing lab work-flows including automation Full-spectrum data you can trust By capturing the complete intrinsic fluorescence spectrum across the full temperature range, SUPR-DSF reveals subtle stability differences others miss. Rich, high-integrity data enables flexible analysis approaches and confident decision-making, even at therapeutic protein concentrations or in challenging formulations. Dye-free, in-plate simplicity SUPR-DSF eliminates extrinsic dyes and extra preparation steps by measuring intrinsic protein fluorescence directly in a single 384-well plate. This reduces hands-on time, lowers error risk, and ensures signals reflect true protein unfolding. Scale stability screening with automation SUPR-DSF fits seamlessly into automated stability screening workflows, enabling labs to scale condition screening and candidate ranking without adding operational complexity. Its plate-based format supports straightforward integration with liquid handlers for repeatable sample setup and consistent execution across large studies. The result is faster iteration, improved reproducibility, and a stability workflow that can grow with your pipeline. Precision DSF technology The SUPR-Suite software provides a comprehensive data analysis environment. Users can process emission spectra via barycentric mean, ratio of two wavelengths, or intensity at a single wavelength range.Automatic peak fitting of the first derivative, combined with interactive tools, ensures that DSF data analysis is both straightforward and efficient. Additionally, integrated thermodynamic models allow for precise fitting to determine van ’t Hoff enthalpies and accurate onset temperatures.
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Don’t just keep up, stay ahead with SX20 Unlock biology in real time. Capture millisecond reactions with unmatched precision. Discovery done right The world’s most-cited stopped-flow system, SX20 gives scientists insight into the fastest molecular events, revealing reaction pathways, intermediates, and mechanisms that shape biological function. With high sensitivity, dual-detection capabilities, and reproducible measurements, it provides reliable data on protein interactions, folding, ligand binding, enzyme kinetics, and chemical reactions—empowering better decision-making in research and formulation development. Gain rich, multi-dimensional data Simultaneously monitor multiple signals—such as fluorescence, absorbance, or FRET—to capture complementary insights in a single experiment. Dual-detection maximizes efficiency and gives you deeper mechanistic understanding faster. High-speed spectral acquisition reveals reaction intermediates in real time. SX20 captures the full evolution of complex mechanisms across the UV–Vis range, enabling global analysis and multi-step kinetic modeling. Simplify complex experiments Automatic dead-time and drive-volume measurements, combined with the integrated pneumatic mixer, reduce setup complexity and eliminate manual calibration—so you can focus on analyzing results rather than troubleshooting the instrument. Flexibility for every experiment Choose from a wide range of light sources, detectors, and accessories, supported by Pro-K software. SX20 adapts to diverse biological and chemical systems, enabling thermal, pH, or chemical stability studies and custom workflows with ease. Light sources designed for your workflow SX20’s interchangeable light sources give researchers the freedom to optimize each experiment. As shown below, the xenon lamp provides substantially higher intensity and signal-to-noise performance for fluorescence polarization kinetics, revealing subtle structural changes with greater precision. LED illumination remains a robust, cost-effective option for routine assays or strongly fluorescent samples. With SX20, you can tailor the setup to the demands of your biology without compromising data quality. Explore key molecular interactions SX20 lets you study fast biochemical reactions in real time, providing actionable insights into enzymes, protein folding, and protein-ligand interaction. Precision kinetics, trusted worldwide SX20 combines rapid stopped-flow mixing, dual detection, and optimized optics to deliver reproducible, high-sensitivity kinetic measurements. Trusted by over 1,000 labs worldwide, it powers 12,000+ peer-reviewed discoveries and is the most-cited stopped-flow system in the world. Capture fast reactions. Reveal molecular insights. Accelerate your kinetic studies with precision, sensitivity, and confidence.
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