An important, yet often overlooked component that can make or break good imaging. At abberior, we take test images with all third-party objective lenses before we install them in your microscope and only the best objective lenses are handpicked for our STED and MINFLUX systems. And since our EASY3D unit is compatible with all types of high-NA objectives, we offer the widest range of lenses for STED of all manufacturers. Oil immersion, water, silicon or glycerol, dipping lenses, and even air immersion – let your sample dictate the objective lens, not your microscope.
The abberior RAINBOW Detection pairs high transmission and quantum efficiency with optimal wavelength discrimination. There are no more compromises: you benefit from both maximum signal and full spectral flexibility! The superior detection efficiency of APDs compared to any other photon detector, including PMTs and hybrid detectors, guarantees that you collect as many precious fluorescence photons from your sample as possible. full contrast, full flexibility Continuously variable spectral detection. Spectral selection with ultra-high transmission A pair of gradient-coated filters are translated within the RAINBOW Detection in order to freely define the edges of the detection window between 400 and 800 nm. Up to four RAINBOW devices can be installed or upgraded at any time and the fluorescence can be freely distributed between every detector. Super-sensitive detection Our standard detectors are avalanche photo detectors (APDs), because they have superior quantum efficiencies, up to a factor of two above hybrid detectors. This means that even when the signal levels are low, our APDs still collect plenty of photons for a meaningful image. Typical applications are superresolution STED imaging and experiments with low labeling densities designed to maintain physiological conditions. At the same time, high-signal samples are perfectly taken care of by DYNAMIC PLUS, our dead-time compensation that yields crisp images of brightly labeled samples. This way, confocal imaging is highly improved, too. Of course, you can opt between quantitative analysis of raw data or mathematical post-processing, e.g. deconvolution. Because we always give you genuine photon counts, you remain in full control of your data interpretation. Always. Other detectors are available on request. - Gap-free spectral detection - Choose any wavelength band between 400 and 800 nm - APDs for the highest sensitivity (up to 65% quantum efficiency) - Free selection of excitation and STED wavelength combinations Because every photon counts
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Two color live-cell confocal and STED image of a mammalian cell expressing a SNAP-tag® OMP25 fusion protein decoration the outer membrane of mitochondria. OMP25 is visualized by our new abberior LIVE 610 SNAP ligand (orange). Tubulin filaments are highlighted with abberior LIVE 550 tubulin (cyan). The SNAP-OMP25 plasmid was a gift from Francesca Bottanelli, FU Berlin.
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The RESOLFT device offered by abberior is a superresolution point scanning system for switchable GFPs. It features the lowest principal light dosages of all superresolution methods and is greatly suited for live-cell imaging. - Fully compatible and integrated - Other options on request
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