Ideal imaging conditions, even in turbid samples like tissue, are every microscopist’s dream. However, specimens are often inhomogeneous, and, what’s more, embedding and immersion media are hardly ever perfectly index-matched. Refractive-index mismatches compromise the focusing capabilities of your microscope and can give rise to low-resolution and poor-quality images. abberior’s RAYSHAPE dynamic aberration correction solves this problem. Using adaptive optics with a deformable mirror, it puts diverted rays back on track, guaranteeing bright and crisp images even in complex samples, from top to bottom and regardless of embedding medium, immersion medium, or specimen. Correction happens dynamically on-the-fly and leaves nothing to desire for your results. dynamic aberration correction Dynamic aberration correction with a deformable mirror over about 200 µm z-range. 140 digital actuators adjust the mirror surface within milliseconds. The deeper you dive into the sample, the more severe the aberrations become, and the more you have to correct. In order to achieve zero aberrations throughout the sample, you therefore need to permanently adjust the amount of correction as focus depth changes. Bye-bye, aberrations!
The abberior RAINBOW Detection pairs high transmission and quantum efficiency with optimal wavelength discrimination. There are no more compromises: you benefit from both maximum signal and full spectral flexibility! The superior detection efficiency of APDs compared to any other photon detector, including PMTs and hybrid detectors, guarantees that you collect as many precious fluorescence photons from your sample as possible. full contrast, full flexibility Continuously variable spectral detection. Spectral selection with ultra-high transmission A pair of gradient-coated filters are translated within the RAINBOW Detection in order to freely define the edges of the detection window between 400 and 800 nm. Up to four RAINBOW devices can be installed or upgraded at any time and the fluorescence can be freely distributed between every detector. Super-sensitive detection Our standard detectors are avalanche photo detectors (APDs), because they have superior quantum efficiencies, up to a factor of two above hybrid detectors. This means that even when the signal levels are low, our APDs still collect plenty of photons for a meaningful image. Typical applications are superresolution STED imaging and experiments with low labeling densities designed to maintain physiological conditions. At the same time, high-signal samples are perfectly taken care of by DYNAMIC PLUS, our dead-time compensation that yields crisp images of brightly labeled samples. This way, confocal imaging is highly improved, too. Of course, you can opt between quantitative analysis of raw data or mathematical post-processing, e.g. deconvolution. Because we always give you genuine photon counts, you remain in full control of your data interpretation. Always. Other detectors are available on request. - Gap-free spectral detection - Choose any wavelength band between 400 and 800 nm - APDs for the highest sensitivity (up to 65% quantum efficiency) - Free selection of excitation and STED wavelength combinations Because every photon counts
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Two color live-cell confocal and STED image of a mammalian cell expressing a SNAP-tag® OMP25 fusion protein decoration the outer membrane of mitochondria. OMP25 is visualized by our new abberior LIVE 610 SNAP ligand (orange). Tubulin filaments are highlighted with abberior LIVE 550 tubulin (cyan). The SNAP-OMP25 plasmid was a gift from Francesca Bottanelli, FU Berlin.
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The RESOLFT device offered by abberior is a superresolution point scanning system for switchable GFPs. It features the lowest principal light dosages of all superresolution methods and is greatly suited for live-cell imaging. - Fully compatible and integrated - Other options on request
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