In this webinar, we demonstrate how replacing these conventional steps with microfluidic tangential flow filtration (TFF), using the µPulse® system, transforms the workflow. Our workflow reduces processing time to a few hours compared to the 2- 3 days required by DEF and freeze-drying, while delivering consistent, higher-quality results across batches. The resulting MNPs yield higher and more stable fluorescence signals, directly translating into more reliable MST-based Kd determinations with lower data variability and reduced sample consumption. The methodology described here was developed on the µPulse v3.
At the heart of these instruments are microfluidic chips - the functional units that deliver exceptional precision, speed, and sustainability from nanoliters to milliliters. Mantis Microfluidic Chip These microfluidic chips use patented microdiaphragms and valves that are pneumatically controlled to deliver metered droplets, enabling precise, non-contact dispensing. The dispensing cycle begins when vacuum is applied to the input valve, drawing a fixed volume of liquid into the microdiaphragm. Pressure then closes the input valve to isolate the measured volume; after which, the output valve opens, and pressure is applied to the diaphragm, which expels the droplet through the nozzle. This innovative design enables the dispensing of fixed discrete volumes, even for challenging liquids such as volatile and viscous solutions. Sustainable, Tipless Dispensing: High-throughput assays consume enormous amounts of plastic tips, incurring both financial and environmental costs. Our microfluidic chips make tipless dispensing the new standard for modern laboratories. Their reusable design cuts consumable cost, while built-in RFID tracking monitors chip usage to ensure consistent performance. Each chip is optimized for reliability and longevity, turning sustainability into a core design principle rather than an afterthought. No Cross-Contamination: In high-value assays, cross-contamination is a critical failure point, causing batch loss, increased costs, and compromised results. Formulatrix microfluidic chips are built to eliminate cross-contamination. Each reagent travels through an isolated fluid path and is dispensed non-contact, preventing reagent carryover between dispenses. The chip is designed for reuse—simply wash it to clear the fluid path of any residual material. Together, these features protect even the most sensitive assays, ensuring the accuracy and reliability of every result. Miniaturization with Precision and Efficiency: As modern workflows move toward smaller reaction volumes, every drop counts. Traditional systems struggle, wasting reagents through dead volume and losing accuracy at sub-microliter scales. Formulatrix microfluidic chips solve these challenges by dispensing volumes down to 100 nL with < 2% CV, even into 384- and 1536-well plates with the dead volume as low as 6 µL . This minimizes waste and maximizes the value of every reagent and enzyme, enabling true assay miniaturization without sacrificing accuracy.
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A compact, programmable dispenser built for workflows where every nanoliter counts from master mix preparation to sensitive cell and bead dispensing. Volume Range Min: 0.1 µL Max: ∞ Technology Micro-Diaphragm Pumps + Continuous Flow Precision CV <2% @ 0.1 µL More Than a Reagent Dispenser While traditional handheld pipettes or larger automated liquid dispensers greatly deteriorate in accuracy and precision below 2 µL volumes, the Mantis automated liquid dispenser excels at low-volume dispensing in a wide variety of scientific workflows. The system is an easy and affordable solution to quickly increase the reproducibility and quality of your research as well as the throughput and efficiency of your laboratory. The diaphragm-based chip design is liquid class compatible, easily dispensing aqueous and organic solutions. Additionally, the Mantis will dispense in any SBS template from 6 to 1536 well plates and can be easily programmed to dispense to custom designs as needed. Reduce Costs - Save on Reagents and Consumables - Miniaturize reactions with tipless dispensing Reproducible and Accurate - Enhanced Precision & Accuracy - Dispense volumes down to 100 nL at CV < 2% Versatile and Flexible - Dispense and Normalize with Ease - Reagent dispensing for a broad range of applications Benefits High Precision and Reproducibility: Dispense volumes as low as 100 nL with exceptional consistency (CV < 2%), enabled by the new High-Fidelity Chips that ensure even tighter control over dispense accuracy and precision Efficient: Miniaturize reactions, scale up throughput, save on reagents, and remove pipette tip cost, saving time and resources across the board Tackle High-Viscosity and Diverse Reagents: The continuous flow chips enable accurate dispensing of thick fluids like 70% glycerol, DMSO, beads, and mastermix—ensuring broad reagent compatibility for various workflows Reliability and Traceability with Every Dispense: The Mantis ensures reliable dispensing with droplet verification, real-time LED status, automated breach detection, and RFID tracking for accurate, traceable results and enhanced uptime across your workflows Fast, Error-Free Setup with CSV Templates: Seamlessly import CSV templates for quick and error-free experiment setup, streamlining workflows and reagent normalization Fast Labware Setup with Intuitive Control: Define labware in seconds with the Interactive Labware Teaching module, and enjoy a streamlined setup process through an intuitive user interface Compatible with a Broader Range of Plates: The Adaptable Plate Clamp is gentle enough to accommodate even thin-bottomed plates Biosafety Cabinet Compatible: With its compact dimensions (350 mm x 232 mm x 212 mm), the Mantis can be used in various biosafety cabinets to accommodate workflows that require this level of safety Automation Friendly: Integrate effortlessly with external systems, fostering connectivity and dat a synchronization Seamless Operation in Regulatory Environments: The system features optional 21 CFR Part 11 Capable Software Small Footprint: All these new features are packed within the same small footprint Broad Range of Applications - Assay Development and Design of Experiment (DoE) - Next Generation Sequencing (NGS) - Library Preparation - Polymerase Chain-reaction (PCR) Workflows Real-time and Digital - Enzyme-Linked Immunosorbent Assays (ELISA) - Cell Culture and Bead-Based Assays - Scaling Up Organoid Cultures - 3D Cell Culture Automated Washing Built-in dual wash stations for easy fluid pathway cleaning following dispenses. QC Droplet Detection Station Verify your dispenses with the new Quality Control Droplet Detection Station Waste Collection Waste Station Sample Numbers and Accessories Automatic Chip Changer (ACC) - Dispense up to 6 reagents to create complex plates and assay development protocols Large Capacity Chip Changer (LC³) - Up to 24 reagents can automatically be dispensed Flexible Reagent Formats Use Any of these Reagent Formats - Pipette Tips - Microfuge Tubes - 15 mL and 50 mL Tubes - Bottles Plate and Sample Types (SBS, Tubes and More) Dispense into 6-, 24-, 48-, 96-, 384-, and 1536-well formats. Able to create custom, non-standard plate templates quickly and easily Accuracy of 'Chips' State-of-the-art Dispensing Technology Accurately and rapidly dispense discrete, or continuous volumes of liquid, - 0.1 μL – 5 μL - CV's < 3% - Continuous flow (600 μL /sec.) Radio-Frequency Identification (RFID) Automatically track all your chip usage data including, diaphragm cycles and dispense history. The RFID chip will also notify you if any reagent is not in the correct position on the system. Compatability Chemical: Dispense DMSO and other harsh solvents using optional perfluoroelastomer chips Hardware: Expands the number of reagents that can be dispensed by adding a modular carousel for up to 24 chips, or 48 different reagents
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Precision meets power with the all-new XL Chip, engineered to deliver metered dispensing of 50 µL, making it the largest-volume diaphragm chip in our Mantis chip catalog. Designed for applications that require higher robustness, scalability, and chemical compatibility, the XL chip represent the next evolution in automated liquid dispensing. Modern chemistry workflows, from reaction setup and compound screening to formulation and sample prep, routinely rely on the dispensing of aggressive solvents and corrosive reagents. Our LV and HV PFE chips have long supported miniaturized, high-precision workflows for harsh reagents. But for workflows that operate at higher volumes and with harsher reagents, a new level of performance was needed. Designed for Chemistry - Built to Scale The XL Chip have been developed specifically to bridge this gap. With a 50 µL metered volume, it enables the accurate dispensing of larger volumes in a single shot, reducing the need for repeated cycles and minimizing wear on the system. Its advanced chemical-resistant fluorinated ethylene propylene (FEP) body and perfluoroelastomer (PFE)diaphragm ensure reliable performance with even the most challenging solvents, for example, DMSO, THF, Toluene, Chloroform, as well as strong acids and bases. Key Benefits - High-volume metered dispensing: (50 µL) in a single actuation- filling 96 well-plate in less than a minute - Exceptional chemical compatibility:Compatible with a vast range of aggressive chemicals - No calibration required: The system is ready to use out of the box for consistent performance - Exceptional precision:CV < 2% at 50 µL for high reproducibility across dispenses - Save on Reagents:Dead Volume as low as 70 µL Whether you're scaling up reaction volumes, formulating compound libraries, or preparing complex mixtures, the XL chip bring a new level of versatility and durability to your chemistry workflows. Revolutionize Your Workflows With the XL Chip, you can now automate higher-volume dispensing with the same accuracy, reliability, and cleanliness that define our PFE chip portfolio. It's not just a bigger chip, it's a more powerful solution for demanding chemical environments.
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Verify your dispenses with the new Quality Control Droplet Detection Station. Enjoy Peace of Mind that Your Dispenses were Precise and Accurate. Optimize your workflow and ensure reliable results with the new Quality Control (QC) Droplet Detection Station. The QC station uses LEDs and optical sensors to confirm proper chip function by detecting the presence of dispensed drops. This technology allows the Mantis to automatically verify if drops are dispensed at the beginning and end of each run, providing both further confidence in dispense quality and traceability. This feature also eliminates the need for manual priming, saving you time and minimizing the risk of errors. With the QC station, you can have complete confidence that your Mantis chips are functioning properly throughout the dispensing process, delivering trustworthy results.
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