TM A Practical Approach to CZE Dynamic vs Bonded Coated Capillaries Application Sheet: CElixir can be fully Automated Precise EOF every run “Fresh” Start every run Minimize “Local Voids” in your CZE ¢¢¢¢¢¢¢¢ The advantages of using a rapid, (dynamic coating) for overcoming the inherent problems of permanently bonded capillaries and their constantly changing morphology are many. Bare fused silica and permanently coated capillaries for HPCE are effected by the CE environment of your run. Parameters such as pH, friction and sample adsorption will cause a loss of the coating and this will degrade efficiency and create poor reproducibility. to the capillary wall is one of the main culprits producing inconsistent CZE. Each time you do a run on a bare fused silica or a bonded-coated capillary, some of your analyte may be adsorbed to the wall. The “local” zeta potential may fall to zero at this particular spot creating a kind of “void” in the flow. With a sufficient quantity of “voids”, the speed of your EOF will change and make quantification and reproducibility very difficult as can occur. When you use the CElixir dynamic coating system instead of a bonded capillary or bare fused silica capillary, in each of your runs, the capillary is conditioned, method is run, then stripped and re coated minimizing wall adsorption effects. Your benefit is very simple, the EOF is consistent from run to run: even capillary-to-capillary. This makes your installed capillary on your CE instrument last longer minimizing the time it takes to change and condition capillaries. CElixir's faster EOF at low pH will further help to minimize band broadening because the faster the separation the less chances samples have to be adsorbed to the wall. and shelf life can be a potential problem for permanently bonded capillaries. Time and improper storage can cause capillaries to dry out and the internal morphology and chemistry can degrade from it original intent. With dynamic coatings you do not have to worry about this problem as your HPCE column is constantly regenerated, each run. If you bond the capillaries yourself to avoid these problems, consistency and time to properly bond them become a disadvantage. With a dynamic coating solution that is manufactured under, you are assured of the same, consistent performance each time. Another advantage that you get from CElixir's dynamic coating system is that the coating can be by adjusting the CElixir Accelerator Solution's (B) pH. With bonded wall coatings this is often not possible and usually the bonded coatings are optimized for one specific method or pH range. in-situ coating method Sample adsorption band broadening Stability GMP Conditions optimized for your method
The most flexible, robust and reproducible Entry-Level Flow FFF system for Research Applications. Application Range The AF2000 AT is the basic modular system which marks the Entry Level of the Postnova AF2000 MultiFlow platform. The system is non-temperature controlled and runs at typical ambient laboratory conditions from 15° to 25°C. It is ideal for standard research applications in university research groups and educational institutions with a clear focus and defined application range. The AF2000 is the best choice and a good starting point for groups which want to enter into the area of Field-Flow Fractionation for the first time. It is available as aqueous metal-free, stainless steel and multisolvent version and can be run with a broad range of aqueous and organic solvents. Planar Asymmetric Flow FFF (AF4) and circular HollowFiber Flow FFF (HF5) channels can used at the same time. Various detection principles, such as UV-Absorbance (UV), Refractive Index (RI), Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) can be coupled easily online. System Hardware It is the Postnova's clear mission to only offer fully optimized and professional FFF hardware, software and support solutions. Therefore, the AF2000 AT series is a basic but completely optimized Flow FFF system which contains all necessary standard modules and components in the package that are required for a robust, reliable and fully operable Flow FFF system. There is no need for assembling and utilizing semi-suited third party HPLC components which are often just made for other purposes, show less flexibility and are not available in the strict specifications needed for FFF applications. The AF2000 AT series is a true ready-to-use non-compromise Flow FFF system where no additional components are required. The system incorporates the complete set of Postnova's proven FFF technologies which makes it the most easy-to-use, robust and reproducible system available. The AF2000 AT system is comprising an Eluent Organizer (PN7205), an Eluent Degasser (PN7520), an Eluent Pump (PN1130), a Focus Pump (PN1130), a Flow FFF Control Module with integrated electronic interface and cross-flow syringe pump (AF2000 Module), a Manual Injector Holder (PN5120), a user-selectable Asymmetric / HollowFiber Flow FFF channel (AF4/HF5) and a Control PC with the NovaFFF software suite for operation of the AF2000 AT system. Additional System Options The AF2000 AT Flow FFF is available in different versions and additionally can be upgraded and customized for special applications by adding extra system options. This can be done at the date of purchase or later as an upgrade. - EFS Eluent Filtration System - UVD Eluent Disinfection Module - SAM Manual/Auto Injector with Options - ELC Aqu. Metal-free/Organic/MultiSolvent - AF4/HF5 Channel Versions and Membranes - S3 Outlet Splitting Sensitivity Enhancer - RID Refractive Index Detection System - UVD Absorbance Detection System - FLD Fluorescence Detection System - MALS Multi-Angle Light Scattering Detector - Online DLS Dynamic Light Scattering Detector - ICP-MS Online Connection and Interface - FRA Automatic Fraction Collector with Options System Software The unique NovaFFF single software platform is used for full control and data acquisition of the entire AF2000 AT Flow FFF together with associated peripheric modules (such as Auto Injector, Fraction Collectors) and detectors (LS, RI, UV, FLD). The software also allows comprehensive data analysis and evaluation based on Relative Standard Calibration and Absolute Light Scattering methods. Furthermore, the software is also the platform to run all major FFF versions, including AF2000 MultiFlow FFF, CF2000 Centrifugal FFF and TF2000 Thermal FFF. This, together with additional features, makes NovaFFF the most advanced and leading software platform available for FFF applications today. Temperature Range: - Room Temperature Principle Measurement Range: - Particles: 1 nm to 1 µm - Polymers: 500 Da to 10 12 Da Channel Dimensions: - Volume: 0.5 -2.5 mL - Size: 335 x 60 x 40 mm - Thickness: 350 µm (other sizes available on request) Carrier Liquids: - Aqueous: any aqueous liquid, pH from 2 - 11 (depending on acid and conditions, please inquire), ionic strength from DI water to saline - Organic: THF, MeOH, etc Membranes: - reg. cellulose, PC, PES, CTA, TF, PAN - Molar Mass: 0,3; 1; 4; 5; 10; 20; 30; 50; 100; 150 kDa (depending on membrane type) Detectors: - UV - RI - Light Scattering - Viscometry - Fluorescence and more - Multiple Detectors in line Channel Flow Range: - 0 - 10 mL/ min Cross Flow Range: - 6.25 µL/min. - 7.5 mL /min Pressure Limit: - 0 - 35 bar Power Requirements: - 110 V or 240 V, 50/ 60 Hz; 2,5 A PC Requirements: - Windows, min. 64 MB RAM, 2 RS232 ports Software System: - NovaFFF AF2000 Control - NovaFFF Analysis Maintenance Parts: - Consumables - Pistons and piston seals - Inline filters - Membranes Injection Volume: - 1 - 1000 µL - Standard 20 µL - Optional 1000 µL Injected Sample Mass: - Typical injection mass 20 - 100 μg Injection Method: - Via manual injection valve - Via PN5300 autosampler
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The most sophisticated Advanced-Level Flow FFF system for Research and QC Applications. Application Range The AF2000 MT is the modular Advanced-Level system of the Postnova AF2000 MultiFlow platform. The system includes sophisiticated temperature control and runs at conditions from 5° to 90°C. This makes the system even more stable due to the temperature stabilized diffusion process. Also separations of fragile biomolecules at lower temperatures as well as high molar mass macromolecules requiring higher temperatures are possible. The AF2000 MT therefore offers the highest reproducibility and the maximum application range within the AF2000 MultiFlow Platform. The system is ideal for special research applications in university, public and industrial research groups and also for high demanding QC applications in production environments. The AF2000 MT is the best choice for advanced FFF groups which want to work at the highest technical level and expect maximum performance from their FFF. The system is available as aqueous metal-free, stainless steel and multisolvent version and can be run with a broad range of aqueous and organic solvents. Planar Asymmetric Flow FFF (AF4) and circular HollowFiber Flow FFF (HF5) channels are standard and can be used at the same time. Various detection principles, such as UV-Absorbance (UV), Refractive Index (RI), Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) can be coupled easily online. System Hardware It is Postnova's clear mission to only offer fully optimized and professional FFF hardware, software and support solutions. Therefore the AF2000 MT series is a completely optimized Flow FFF system, which contains all necessary standard modules and components in the package, that are required for a robust, reliable and stable operable Flow FFF system. There is no need for assembling and utilizing semi-suited third party HPLC components, which are often just made for other purposes, show less flexibility and are not available in the strict specifications needed for FFF applications. The AF2000 MT series is a true ready-to-use non-compromise Flow FFF system, where no additional components are required. The system incorporates the complete set of Postnova's proven FFF technologies, such as FocusTech, ChannelCartridge and SyringeXFlow, which make it the most easy-to-use, robust and reproducible system available. The AF2000 MT system is comprising an Eluent Organizer (PN7205), an Eluent Degasser (PN7520), an Eluent Pump (PN1130), a Focus Pump (PN1130), a Flow FFF Control Module with integrated electronic interface and cross-flow syringe pump (AF2000 Module), a FFF Channel Thermostat with Manual Injector Holder (PN4020), a user-selectable Asymmetric / HollowFiber Flow FFF channel (AF4/HF5) and a Control PC with the NovaFFF software suite for operation of the AF2000 MT system. Additional System Options The AF2000 MT Flow FFF is available in different versions and additionally can be upgraded and customized for special applications by adding extra system options. This can be done at date of purchase or later as an upgrade. - EFS Eluent Filtration System - UVD Eluent Disinfection Module - SAM Manual/Auto Injector with Options - ELC Aqu. Metal-free/Organic/MultiSolvent - AF4/HF5 Channel Versions and Membranes - S3 Outlet Splitting Sensitivity Enhancer - RID Refractive Index Detection System - UVD Absorbance Detection System - FLD Fluorescence Detection System - MALS Multi-Angle Light Scattering Detector - Online DLS Dynamic Light Scattering Detector - ICP-MS Online Connection and Interface - HCT Multi Heated Capillary Thermostat - FRA Automatic Fraction Collector with Options System Software The unique NovaFFF single software platform is used for full control and data acquisition of the entire AF2000 MT Flow FFF together with associated peripheric modules (such as Auto Injector, Fraction Collectors) and detectors (LS, RI, UV, FLD). The software also allows comprehensive data analysis and evaluation based on Relative Standard Calibration and Absolute Light Scattering methods. Furthermore the software is also the platform to run all mayor FFF versions, including AF2000 MultiFlow FFF, CF2000 Centrifugal FFF and TF2000 Thermal FFF. This together with additional features, makes NovaFFF the most advanced and leading software platform available for FFF applications today. Temperature Range: - Cooling real 5°C - Heating max. 90°C depenting on channel and frit material (PMMA channel up to 70°C, with ceramic frit up to 40°C) Measurement Range: - Particles: 1 nm to 1 µm - Polymers: 500 Da to 10 12 Da Channel Dimensions: - Volume: between 0.5 - 2.5 mL - Size: 335 x 60 x 40 mm - Thickness: 350 µm (other sizes available on request) Carrier Liquids: - Aqueous: any aqueous liquid, pH from 2 - 11 (depending on acid and conditions, please inquire), ionic strength from DI water to saline - Organic: THF, MeOH, etc Membranes: - reg. Cellulose, PC, PES - Molar Mass: 0,3; 1; 2; 4; 5; 10; 30; 100 kDa Detectors: - UV - RI - Light Scattering - Viscometry - Fluorescence and more - multiple detectors in line Channel Flow Ranges: - 0 - 10 mL/ min Cross Flow Range: - 6.25 µL/min - 7.5 mL/ min Pressure Limit: - 0 - 35 bar Power Requirements: - 110 V or 240 V, 50/60 Hz; 2,5 A PC Requirements: - Windows, min.64 MB RAM, 2 RS232 ports Software System: - NovaFFF AF2000 Control - NovaFFF Analysis Maintenance Parts: - consumables - pistons and piston seals - inline filters - membranes Injection Volume: - 1 - 1000 µL - Standard 20 µL - Optional 1000 µL Injected Sample Mass: - Typical injection mass 20 - 100 µg Injection Method: - Via manual injection valve - Via PN5300 autosampler
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We provide Fused Silica Capillaries for CE applications with inner diameters from 50 – 150 μm and an outer diameter of 365 μm. These capillaries come in various lengths depending on the customer’s needs. Range of applications: • Capillary electrophoresis with tubing and windowed capillaries • Precision flow cells with custom tubing for CE
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