The software tool GATTAnalysis by GATTAquant provides a fast and easy way to evaluate super-resolution data gained from measurements with DNA-origami based nanorulers. Just by a few clicks origami structures can be detected in the super-resolved image and analyzed with regard to their dye-to-dye-distance. The resulting distance values are subsequently presented in a histogram including basic statistical analyzing options and can be exported to a text file. The analysis of data gained from microscopy with intrinsic binning (STED, SIM, etc.) differs slightly from those gained from localization based microscopy (STORM, PALM, DNA-PAINT, etc.). The processing of both data types will be presented independently in the subsequent chapters. The software is based on Java and requires the Java SE Runtime Environment 7 or higher.
Nanorulers from the GATTA-PAINT series are the ideal resolution rulers for any type of localization based super-resolution microscopy. Due to the mechanism for providing the blink kinetics which is based on the DNA PAINT technology the samples are nearly free of photo bleaching. In addition the design containing three emitting points allows the acquisition of very eye-catching images. We offer for the GATTA-PAINT nanorulers the mark-to-mark distances in the sizes 40 nm and 80 nm. Both sizes are available in the colors red (ATTO 655), green (ATTO 542) or yellow (Cy3B) as well as in the color combination red/green (ATTO 655 & ATTO 542) or red/yellow (ATTO 655 & ATTO Cy3B). product properties of GATTA-PAINT 80 nm Nanoruler - PAINT 80R: Color red; Available fluorophores ATTO 655; Recommended laser source 625 - 665 nm; Surface density ≈ 1/µm²; Distance 2x 80 nm; Level Beginner; Price 365,-€ - PAINT 80Y: Color yellow; Available fluorophores Cy3B; Recommended laser source 560 – 590 nm; Surface density ≈ 1/µm²; Distance 2x 80 nm; Level Beginner; Price 365,-€ - PAINT 80G: Color green; Available fluorophores ATTO 542; Recommended laser source 530 - 560 nm; Surface density ≈ 1/µm²; Distance 2x 80 nm; Level Beginner; Price 365,-€ product properties of GATTA-PAINT 40 nm Nanoruler - PAINT 40R: Color red; Available fluorophores ATTO 655; Recommended laser source 625 - 665 nm; Surface density ≈ 1/µm²; Distance 2x 40 nm; Level Advanced; Price 365,-€ - PAINT 40Y: Color yellow; Available fluorophores Cy3B; Recommended laser source 560 – 590 nm; Surface density ≈ 1/µm²; Distance 2x 40 nm; Level Advanced; Price 365,-€ - PAINT 40G: Color green; Available fluorophores ATTO 542; Recommended laser source 530 - 560 nm; Surface density ≈ 1/µm²; Distance 2x 40 nm; Level Advanced; Price 365,-€ Beginner: Samples to check the basic functions of your microscope. Easy to resolve, also for beginners in the field of microscopy. Advanced: Samples to optimize the settings of your microscope. Advanced experience is necessary in order to resolve the nanorulers. fluorophore properties - ATTO 655: Our notation R; Excitation 655 nm; Emission 680 nm - Cy3B: Our notation Y; Excitation 560 nm; Emission 571 nm - ATTO 542: Our notation G; Excitation 542 nm; Emission 562 nm
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The GATTAquant Brightline series delivers you a much better tool for your experiments and calibration measurements we now developed a new series of GATTA-SIM nanorulers featuring an even higher fluorescence brightness but without any loss of compactness compared to previous GATTA-SIM nanorulers. Currently we provide as colors red (ATTO 647N), yellow (Alexa Fluor 568), and blue (Alexa Fluor 488). If you could not find the new GATTA-SIM - brightline nanorulers with your required distance please add a note to your request and we are happy to offer you custom samples optimized for your application.
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The GATTAquant Brightline series delivers you a much better tool for your experiments and calibration measurements we now developed a new series of GATTA-SIM nanorulers featuring an even higher fluorescence brightness but without any loss of compactness compared to previous GATTA-SIM nanorulers. Currently we provide as colors red (ATTO 647N), yellow (Alexa Fluor 568), and blue (Alexa Fluor 488). If you could not find the new GATTA-SIM - brightline nanorulers with your required distance please add a note to your request and we are happy to offer you custom samples optimized for your application.
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product properties of GATTA-SIM - brightline 140 nm and 120 nm Nanoruler SIM 140Y - brightline: Color yellow; Available fluorophores ATTO 565; Recommended laser source 540 - 585 nm; Surface density ≈ 1/µm²; Distance 140 nm; Level Advanced; Price 365,-€ SIM 140B - brightline: Color blue; Available fluorophores Alexa Fluor® 488; Recommended laser source 480 - 505 nm; Surface density ≈ 1/µm²; Distance 140 nm; Level Advanced; Price 365,-€ SIM 120B - brightline: Color blue; Available fluorophores Alexa Fluor® 488; Recommended laser source 480 - 505 nm; Surface density ≈ 1/µm²; Distance 120 nm; Level Expert; Price 365,-€
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