By Institute for Advanced Chemistry of Catalonia (IQAC-CSIC)
Hair Efficacy Test - Degradative treatments of hair: perming, bleaching, relaxation, UV photodegradation, etc. - Extraction and analysis of hair lipids (TLC-FID) - Hydrophobicity / hydrophilicity Determination of contact angle (Electrobalance KSV Sigma 70) - Moisture content. Thermogravimetric analysis (TGA TG-50) (Thermal Analysis Service, SAT) - Absorption/desorption kinetics (DVS) to determine hair permeability (TA Thermobalance). (Thermal Analysis Service, SAT) - Thermotropic Evaluation with differential scanning calorimetry (DSC) to determine the thermal stability of the main morphological components of hair (DSC-821). (SAT) - Mechanical properties of hair such as elasticity, deformation, and energy at break, etc., Dynamometer Instron 5500R - Visualization of the hair surface by Scanning Electron Microscopy (SEM) in collaboration with the Microscopy Service of the University of Barcelona - Color determination (Datacolor 400 Spectrophotometer) - Evaluation of protein degradation of hair after UV irradiation (Bradford colorimetric assay) - Evaluation of free radicals formed in the oxidative process (Electron Paramagnetic Resonance (EPR) Spectrometer EMX-Plus 10/12 Bruker BioSpin) (Magnetic Resonance Service) - Lipid peroxidation of hair lipids. Determination of malonylaldehyde content using the barbituric acid test (TBARS) - Gloss evaluation (Micro-TRI-gloss Glossmeter) - Tryptophan Degradation.(Spectrofluorimetric measurements)