By Institute for Advanced Chemistry of Catalonia (IQAC-CSIC)
The Nuclear Magnetic Resonance (NMR) Service is part of the scientific-technical services of The Institute for Advanced Chemistry of Catalonia (IQAC-CSIC) and is a research support service that manages the facilities, equipment, financial resources, and human resources necessary to obtain NMR spectra. The equipment and services are accessible to researchers from IQAC, as well as from other CSIC institutes or external public centers and private companies. The NMR Service offers both scientific and technical advice on any topic or issue related to Solution Nuclear Magnetic Resonance Spectroscopy. Currently, the NMR Service has two spectrometers and two staff members with specialized scientific-technical training in NMR. The NMR laboratory is located on the ground floor of the Pascual Vila building of the Research and Development Center (CID-CSIC). The configuration of the two spectrometers (liquid probes) requires that samples be in solution. Spectra of a wide variety of samples can be acquired, such as small organic molecules, polymers, inorganic metal complexes, organometallic compounds, peptides, proteins, nucleic acids, carbohydrates, mixtures, samples of environmental origin, etc. Among the wide range of experiments that can be performed, we can highlight: - High-resolution 1D and 2D NMR experiments for the structural characterization of molecules of chemical origin. - Acquisition of one-dimensional NMR spectra of 1H, 13C, 31P, 15N, 19F, 11B, or other nuclei. - Two-dimensional homonuclear (COSY, NOESY, TOCSY, ROESY) and heteronuclear NMR spectra with direct detection (HETCOR, HOESY, J spectra) or inverse detection (HMQC, HSQC, HMBC). - The most common techniques are included (selective excitation, decoupling, suppression of water/non-deuterated solvent resonances, etc.). - Other routine studies such as mixture/formulation analysis, determination of diffusion coefficients, and dynamic NMR. - Quantitative analysis to determine purity and composition, quality control and/or characterization of raw materials. - Structural characterization of macrobiomolecules and their interaction with small molecules - Double/triple resonance experiments for the assignment of peptides/proteins (<20 kDa). - Drug screening by NMR and Structure-Activity Relationship (SAR) studies. - Analysis of reaction kinetics and mechanisms by NMR. - Biological activity studies, such as enzymatic activity, in-cell NMR studies, analysis of cell and tissue extracts (metabonomics by NMR). Users can access the service’s capabilities in self-service mode (internal users) or through experiments performed by Service staff (internal and external users). For more information regarding the possibilities of the NMR technique and access modes, you can contact us by email servei.rmn@iqac.csic.es. The NMR Service uses a variety of software applications for data processing and report preparation: TOPSPIN, MestRENova, MSpin, CCPN, Bruker Amix/BBIOREFCODE, ChemOffice, …
