Structural & Compositional Analysis

Mineral Geological Analysis

Elemental Composition for exploration Mineral Type identification & quantification Geological Material Identification or verification GC-FID Analysis (Gas Chromatography with Flame Ionization Detection)

Material Characterisation

Surface Morphology (imaging) Elemental Mapping (elemental distribution) Component metal quality comparison Metal grade determination Metal Heat treatment condition determination Failure analysis Diamond identification including micro diamonds

Mineral & Geological Testing

Elemental Composition for exploration Mineral Type identification & quantification Geological Material Identification or verification GC-FID Analysis (Gas Chromatography with Flame Ionization Detection)

Polymorph screening

Polymorph screening helps to identify all relevant forms of an API, investigate its properties and choose the optimal crystalline form. Conducting these screens can also help prevent the appearance of new forms in the latter stages of development. Our scientists focus on establishing the interconversions between forms, relative stability of forms and understanding the monotropic or enantiotropic relationship between polymorphs as per the requirement. About 90% of drug substances have the ability to exist in different crystal forms, therefore, understanding and controlling polymorphism is essential to successful pre-clinical development. Understanding polymorphism is key to eliminating batch-to-batch inconsistency and ensuring consistent in-vivo performance. With polymorphs differing in properties such as stability, hygroscopicity, and dissolution rate, some compounds can prove challenging to develop. Polymorph screening is a regulatory requirement whether a salt, cocrystal or free form compound is chosen for development. We recommend performing drug polymorphism screening as part of the process of selecting a pre-clinical candidate. This allows any challenges to be identified and overcome before causing any delays to the pre-clinical program.

Co-crystal screening

Co-crystal screening can identify and assess the potential co-crystal forms of an API and provides an alternative approach to modify the physical properties of an API where salts do not achieve the desired performance or are not an option. With state-of-the-art characterization techniques and rich experience in polymorphism screening, scientific experts at Jubilant Biosys can guide through the process of identifying hydrates, solvates, and meta-stable forms of compounds. Our expertise in crystallization enables us to design scalable methods to reliably produce optimum solid form and provide non-GMP material for pre-clinical studies, supporting progression into contract development and manufacturing organization programs.

Physicochemical Study

Melting point pKa Optical rotation Solid state analysis (DSC, TGA and XRPD) KF Titration Solubility Log P determination Elemental analysis Polarimetry IR Spectroscopy

Structure Elucidation

Structure elucidation of new chemical entities (NCE’s), impurities, degradation products, metabolites and natural products. X-Nuclei NMR (1 H,2 H,7 Li,11 B,13 C,15 N,19 F,23 Na,27 Al,29 Si,31 P,119 Sn,195 K) Structure elucidation by LC-HRMS, NMR, FT-IR & UV-Visible. NMR special experiments (2D-NOESY, COSY, HMBC, EDHSQC, Selective Gradient 1D-NOESY / ROESY, TOCSY, 2D-ROESY, DOSY, H2BC, etc).

Analytical Chemistry Services

Jubilant Biosys provides advanced analytical chemistry services as part of its R&D offerings, including quantitative and qualitative analysis, structural confirmation, chemical characterization, and chiral/achiral purification, delivered with precision, accuracy, and speed. The analytical team provides support to discovery and process chemistry groups as an integrated service or a stand-alone option for our customers. Our unique approach allows us to identify and evaluate the chemical in our analytical testing laboratories rapidly and effectively communicate the resulting data to help our customers understand the product’s chemistry. Our analytical expertise supports small molecules as well as advanced modalities, including oligonucleotide synthesis programs.

X-ray Crystal Structural Studies Services

The Jubilant Biosys team has demonstrated its strength in protein engineering to suit the needs of crystallization by designing multiple constructs for a given gene-to-structure project and worked on ~40 different protein targets and delivered more than 250 co-crystal structures (better than 2.5Å resolution). The structural work has been showcased through publications in peer-reviewed international journals (where the Biosys team is co-authored with clients). - Protein crystallization(apo or in presence of ligand) crystallization screening using commercial kits. - Crystal hit optimization (sparse matrix in-house grid, additive and detergent screening). - Diffraction screening and data collection using in-house x-rays. - Synchrotron beam access (at Australia). - Structure determination. - Structural analysis. - Evaluation of electron density maps for ligand binding.

Mass Spectrometry Proteomics

Traditional methods for mass spectrometry-based proteomics, such as SILAC (stable isotope labeling with amino acids in cell culture), are insensitive, poorly reproducible, costly, and time consuming. LifeSensors’ TUBEs bind to all polyubiquitin chains with 1-10 nM affinity, overcoming major problems common to mass spectrometry proteomics.