Biophysical Facility at Jubilant Biosys provides highly sensitive, label-free and real-time assessment of biomolecular interaction through the Surface Plasmon Resonance (SPR) technology/platform. Our SPR services are carried out by our experienced scientific team that guides you through your project for various applications ranging from small molecule binding kinetics (reversible/irreversible), fragments screening, protein aggregation, drug competition assay etc. With extensive knowledge in the drug discovery process, Jubilant Biosys offers tailored SPR solutions to complex challenges, supporting small molecules and biologics, including antibody drug conjugate programs, to help deliver successful results and achieve our mission. An optical phenomenon, SPR, enables binding activity monitoring of any two unlabelled molecules in real-time. The generated signal is dependent on changes in the refractive index at the surface of a gold sensor chip, as an analyte flows in a microfluidic channel and binds to a ligand immobilized on the sensor chip. Consequently, over time, plotting the binding response vs time called sensorgram. The sensorgram aids in the calculation of binding parameters, such as K D, k on and k off. Advantages of SPR Technology - Deeper insight and understanding of molecular interactions – Real-time monitoring. - Small sample sizes. - Replicate measurements consistently. - Label-free detection. - Reusable sensor chips. Analysis for which the SPR services can be applied for On top of the kinetic constants, SPR measurements can be applied for: - Determination of affinity. - High Throughput Screening of Fragments. - Assessment of irreversible compounds. - Competition assay.
Compiled from https://www.jubilantbiosys.com
