By Melbourne Centre for Nanofabrication
Hyperspectral imaging performs a similar role to microspectrometry, but images a much larger area to provide information on a process within its larger context. It covers a wide spectral range of 400nm to 1000nm in the one grating, by producing a line-by-line scan and reconstructing them into an image. Users may wish to start with hyperspectral imaging to characterise a wide area at a lower resolution, after which they can select a smaller region of interest and compare spectral properties in different parts of the same image. The CytoViva Hyperspectral Imaging System combines brightfield, darkfield and fluorescence in the one instrument. The darkfield condenser is specially designed to offer up to 100nm lateral resolution. This system offers widefield 3D deconvolution, enabling applications such as localisation of nanoparticles in cells.
