Live cell imaging is used in virtually all areas of molecular biology. It places unique demands on imaging conditions and microscope. Controlling the environment of the sample is pivotal to ensure physiological conditions during imaging: temperature, humidity, and CO 2 concentration need to be kept constant. Furthermore, a good autofocus unit is a must, and scanning and detection have to work fast to resolve dynamic cellular processes in time. The microscope should be equipped with a water immersion objective and possibly also a dipping objective. Fluorescence lifetime imaging (FLIM) is a plus. And to facilitate longer measurements and time-lapse microscopy, it’s key to keep the light dose as low as possible to avoid phototoxic effects that might damage the sample. Last but not least, you need to use membrane-permeable, non-toxic, and at the same time bright fluorescent dyes. No matter whether you want to image in confocal or STED mode – abberior has the solution for your live cell imaging experiment. With us, you will find the ideal dye, a suitable microscope system, and the matching technical modules for your purpose; all with the aim of recording the best possible data while treating the sample with care.
