By CytoViva
Using CytoViva enhanced darkfield illumination integrated with hyperspectral imaging, we have successfully identified the presence of QDs both visually and spectrally in retinal tissue (Fig. 1). While we can easily visualize the red emission from the QDs, hyperspectral imaging allows for quantitative measurement of the QD emission. In this case, the emission was measured at 628 nm. This technique has an advantage over confocal microscopy because the QDs can be simultaneously visualized (Fig. 1A) and spectrally corroborated (Fig. 1B) in the context of the tissue (or other matrix) without the use of fluorophores. In the realm of materials science, CytoViva enhanced darkfield hyperspectral microscopy can be used to identify QDs on substrates. In Fig. 2, gold nanoparticles (AuNPs) and QDs were identified and differentiated within a polyester fiber. While we can visualize the nanoparticles embedded in the fiber, we cannot be certain of the nature of the nanoparticles without quantitative confirmation. In a matter of minutes, we acquired a hyperspectral image (Fig. 2A) and determined the identity of the particles from the spectral data (Fig. 2B). Whether you are working with QDs or other nanoscale particles in biological or material matrices, CytoViva enhanced darkfield hyperspectral microscopy can accelerate your research through quick, quantitative data acquisition and minimal sample preparation. Please contact us at info@cytoviva.com to learn more about the technique or to discuss test imaging of your samples.
