By Melbourne Centre for Nanofabrication
Microscopectrometry is able to capture high resolution spectral information for nanoparticles with PMT resolution up to.05nm. It uses a deep-cooled camera to reduce background noise and increase sensitivity. A unique feature of microspectrometry is its ability to measure a wide spectral range - 190nm to 1000nm with high spectral resolutions - and stitch together images from different wavelengths to provide a complete high resolution spectral curve. It performs these scans at three different gratings (150g/mm and 1200g/mm with two different centre wavelengths). Microspectrometry is useful for the imaging of small areas, such as gold nanoparticles, to measure their dynamics and see how they behave in certain environments. The Nikon Ti-U Microscope is combined with a Princeton Instrument Isoplane 320 High performance Spectrograph as well as a Deep Cooled EMCCD camera. It offers transmitted and reflected brightfield illumination and transmitted darkfield illumination.