The Ubiquitin Proteasome System (UPS) controls the principal functions of almost all the cellular proteins of human cells and failures in this system often contribute directly or indirectly to the pathogenesis of many diseases, including cancer, inflammation, and neurodegeneration. There are ~100 deubiquitinases (DUBs) encoded in human genome and the functions of most of these DUBs remain unknown. Identification of substrates of individual DUBs is an essential step to unravel the cellular functions of DUBs. Not only limited to DUBs, but there are also ~700 E3 ubiquitin ligases encoded in human genome and the functions of most of these E3s remain unknown. Recent development of PROTAC drugs to recruit E3 ligases and degrade therapeutic proteins highlight the value of studying E3 ligases. Substrate identification in individual E3 ligases is an essential step to unraveling cellular functions of E3s and identifying novel therapeutic targets. LifeSensors can help you identify the substrates of your preferred E3s and DUBs. Advantages of Choosing LifeSensors For Your Drug Discovery Project In Vitro & In Cell Assays Comprehensive assays to identify DUB & E3 substrates in controlled environments and cellular contexts TUBEs for Detection Advanced TUBE technology enables identification of direct deubiquitination targets Substrate Trapping & Proteomics State-of-the-art proteomic analysis to identify endogenous substrates with precision Orthogonal Validation Rigorous validation of substrates through independent orthogonal assay methods
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