Bacterial nanowire mystery solved

Deep in the ocean or underground, where there is no oxygen, Geobacter bacteria “breathe” by projecting tiny protein filaments called “nanowires” into the soil, to dispose of excess electrons resulting from the conversion of nutrients to energy.

These nanowires enable the bacteria to perform environmentally important functions such as cleaning up radioactive sites and generating electricity. Scientists have long known that Geobacter make conductive nanowires – 1/100,000 the width of a human hair – but to date no one had discovered what they are made of and why they are conductive.

A new study by researchers at Yale, University of Virginia and the University of California at Irvine published April 4 in the journal Cell reveals a surprise: the protein nanowires have a core of metal-containing molecules called hemes.

Previously nobody suspected such a structure. Using high-resolution cryo-electron microscopy, the researchers were able to see the nanowire’s atomic structure and discover that hemes line up to create a continuous path along which electrons travel.

“This study solves a longstanding mystery of how nanowires move electrons to minerals in the soil,” said lead author Nikhil Malvankar, assistant professor of molecular biophysics and biochemistry at Yale and a faculty member at the Microbial Sciences Institute.

“It is possible we could use these wires to connect cells to electronics to build new types of materials and sensors.”

Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers

Fengbin Wang, Yangqi Gu, J. Patrick O’Brien, Sophia M. Yi, Sibel Ebru Yalcin, Vishok Srikanth, Cong Shen, Dennis Vu, Nicole L. Ing, Allon I. Hochbaum, Edward H. Egelman, Nikhil S. Malvankar

Cell Vol. 177, Issue 2, p361-369

DOI: https://doi.org/10.1016/j.cell.2019.03.029

Contact information:
Nikhil Malvankar

Assistant professor of molecular biophysics and biochemistry at Yale
nikhil.malvankar@yale.edu

Tel: 203-737-7590/8001

Fax: 203-737-6715

Lab: Malvankar Lab