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3D-printed pill samples gut microbiome to aid diagnosis and treatment


Bacteria in the gut are pulled into the helical channels by an osmotic 'pump' in the pill. @ Nano Lab, Tufts University

A research team led by Tufts University engineers has developed a 3D printed pill that samples bacteria found in the gut -- known as the microbiome -- as it passes through the gastrointestinal tract (GI). The ability to profile bacterial species inhabiting the gut could have important implications for conditions that affect and are affected by the intestinal microbiome, according to the researchers.


The 3D printed pill described in the journal Advanced Intelligent Systems represents the first non-invasive diagnostic tool capable of providing a profile of microbiome populations throughout the entire GI tract, according to the researchers. Current methods of sampling the microbiome involve primarily the analysis of fecal DNA and metabolites, but that approach provides little information of the environment upstream of the distal colon, where bacterial species can vary significantly.


The pill has been studied extensively in vitro and in vivo, and found to provide accurate identification of bacterial populations and their relative abundance, the paper says. It has been tested in pigs and primates, yet clinical trials will be needed to determine if the pill can be used routinely in humans for clinical care.


More than 1,000 species of bacteria inhabit the healthy gut. The vast majority of these bacteria have a beneficial, supportive role in digestion and protection against disease. When the natural balance of the microbiome is disturbed, a condition called "dysbiosis" occurs, which can be associated with inflammation, susceptibility to infections, and even the exacerbation of other diseases such as cancer. Research is increasingly unveiling specific microbiome metabolites that have beneficial or protective effects against disease.


"We are learning quite a lot about the role of gut microbiome in health and disease. However, we know very little about its biogeography," said Sameer Sonkusale, professor of electrical and computer engineering at Tufts University's School of Engineering and corresponding author of the study. "The pill will improve our understanding of the role of spatial distribution in the microbiome profile to advance novel treatments and therapies for a number of diseases and conditions."


The pill is more sophisticated than just a sponge. It is manufactured in a 3D printer with microfluidic channels that can sample different stages of the GI tract. The surface of the pill is covered with a pH sensitive coating, so that it does not absorb any samples until it enters the small intestine (bypassing the stomach) where the coating dissolves. A semi-permeable membrane separates two chambers in the pill - one containing helical channels that take up the bacteria and the other containing a calcium salt-filled chamber. The salt chamber helps create an osmotic flow across the membrane which pulls the bacteria into the helical channels. A small magnet in the pill enables one to hold it at certain locations in the gut for more spatially targeted sampling using a magnet outside the body. A fluorescent dye in the salt chamber helps locate the pill after it exits the GI tract.


"The design of this device makes it incredibly easy to use, posing little risk to the subject, yet providing so much information," said Giovanni Widmer, professor of infectious diseases and global health in Tufts Cummings School of Veterinary Medicine, and co-author of the study responsible for testing the pills effectiveness in animals studies and for high-throughput sequencing the microbiome samples.


The researchers see this technology as bridging an important gap in understanding the complexity of the ecosystem of the gut. "We have advanced technologies to analyze bacterial populations using DNA sequencing, but until now have not had a way to sample bacteria throughout the GI tract in a way that is not invasive," said Hojatollah Rezaei Nejad, a post-doctoral fellow studying novel applications of 3D printing in Sonkusale's laboratory at Tufts and lead author of the study. "By sampling non-invasively, this pill could help us better identify and understand the role of different intestinal bacterial species in health and disease."


Ingestible osmotic pill for in-vivo sampling of gut microbiome

Nejad H.R., Oliveira B.C.M., Sadeqi A., Dehkharghani A., Kondova I., Langermans J.A.M., Guasto J.S., Tzipori S, Widmer G., Sonkusale S.R.

Advanced Intelligent Systems July 19 2019

DOI: 10.1002/aisy.201900053


Contact information:

Sameer Sonkusale

Professor of electrical & computer engineering at Tufts University's School of Engineering

sameer@ece.tufts.edu

Nano Lab


Giovanni F. Widmer

Professor of infectious diseases & global health in Tufts Cummings School of Veterinary Medicine

giovanni.widmer@tufts.edu

Tel: 508-839-7944


Tufts University

 

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