Reveal all classes of structural variants and leave the limits of traditional cytogenetics and sequencing behind with Optical Genome Mapping (OGM) and the Bionano Stratys™ System. Stratys System Brochure: Explore the Stratys system and get a deeper understanding of its capabilities, range of applications, workflow and more. Stratys Performance Target Effective Coverage 1: 100x, 200x, 400x, 1200x 2 Expected VAF for SV Detection: ≥ 50%, ≥ 20%, ≥ 5%, lower than 5% 4 Stratys Throughput Per Week 3 / Samples: 24/7 operation: 260, 40 hour operation: 120; 24/7 operation: 168, 40 hour operation: 60; 24/7 operation: 96, 40 hour operation: 60; 24/7 operation: 30, 40 hour operation: 24 Imaging Time (One batch of 12 chips): 6 – 10 hours, 12 – 16 hours, 18 – 22 hours, 60 – 70 hours 1 Target Effective Coverage calculated based on 80% map rate achieved 2 Requires Stratys Plus Chip, VAF: Variant Allele Fraction 3 Maximum throughput on one Stratys system; assuming that enough samples are available to run at 100% capacity. Typical performance based on various human control samples run at Bionano. 4 Refer to LIT-00017 Redefining Cell Characterization and QC for Genomic Integrity and Off-Target Monitoring and ask your Bionano contact for more details. Lower VAF percentages can be achieved when using the Stratys Plus Chip, which enables up to 1200x genome coverage.
A superior automated approach to DNA and RNA isolation for FFPE samples. Isolate High-Quality Nucleic Acids from Challenging Samples The Ionic system uses isotachophoresis (ITP) to isolate, purify, and concentrate genomic DNA and RNA from cells, tissue, and FFPE samples with Ionic G2 chemistry. DNA and RNA samples prepared by the system require just a few minutes of hands-on time per sample. Simplify Your Nucleic Acid Purification Using the Fully Automated Workflow The Ionic system outperforms most nucleic acid extraction and purification methods: - Higher nucleic acid yields: No sample loss associated with binding nucleic acids to, or stripping from, fixed surfaces - Simple, automated workflows: No columns or beads and no repetitive washing - Reduced nucleic acid fragmentation: No harsh high-salt buffers or organic solvents Next Generation Sample Preparation System with the Easy-to-use Ionic System Chip By applying an electric field across the length of the Ionic Chip microchannels, the Ionic system separates and concentrates nucleic acid between buffers with higher and lower mobilities. Impurities fall behind the low mobility buffer and are separated from target nucleic acids. As target nucleic acids pass through the channel, an integrated sensor stops the current once nucleic acids reach the extraction well. The workflow requires no binding, stripping, or washing from fixed surfaces for higher yields, higher quality nucleic acids, and ultimately, better data for your research. - No organic solvents - No harsh, high-salt buffers - No system programming - No beads or repetitive washing - No pumps, valves, or other moving parts A Superior Approach to Column-Based Purification Isotachophoresis (ITP) separates and concentrates charged molecules in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Ionic Fluidic Chip. An electric field is then applied to the chip and the nucleic acid is isolated in its natural, native form. The nucleic acid is not denatured or dehydrated, and there's no binding to, or stripping from, fixed surfaces. The result is a higher yield of pure nucleic acid that is less fragmented and free from bead or wash buffer contamination. Superior Nucleic Acid Recoveries from Cells and FFPE Samples Extract Up to 4.5x More Nucleic Acid with Ionic G2 A vast majority of clinical samples used in oncology research are stored as FFPE tissues, which often contain degraded or fragmented nucleic acid. Conventional extraction methods are labor intensive and can further damage nucleic acid during the extraction and purification process. The Ionic system simplifies and accelerates nucleic acid purification, resulting in higher yields of higher quality DNA. Simple, Automated Workflow Minimizes Hands-on Time and Reduces Errors A simplified workflow minimizes opportunities for material loss and errors. The FFPE protocols greatly simplify the processing to 3 minutes per sample and enables working directly from scrolls. Nucleic Acid Purification Workflow Comparison The Isotachophoresis workflow consolidates and eliminates multiple steps compared to column-based or bead-based methods.
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Reveal genome-wide structural variation with the Saphyr™ optical genome mapping system. Achieve Unparalleled Structural Variant Detection. Routinely detect all classes of structural variants down to 500 base pair (bp) resolution and 5% variant allele frequency (VAF) with optical genome mapping (OGM) using the Saphyr™ system. Saphyr™ is the most powerful structural variant detection tool available, detecting genomic variants commonly missed by next-generation sequencing (NGS) technologies and conventional cytogenetic techniques. Empower your lab with advanced SV detection using optical genome mapping. The Saphyr™ system provides optical genome mapping of ultra-high molecular weight (UHMW) DNA in its native state, from molecules 150 kbp to multi-megabase pairs in length. In as little as three days, complete the entire workflow from sample to structural variation call using the Bionano sample preparation kits, Saphyr™ system, and software. Achieve broad, unbiased genomic coverage with flexible data collection. The genomic coverage achieved with the Saphyr™ system is flexible and allows for the detection of heterozygous variants or rarer variants found in mosaic samples and heterogeneous tumors. Collect 100X coverage of a human genome in as little as six hours. See deeper and rarer variants by simply extending the data collection time on Saphyr™ at no additional consumables cost. Achieve 400X coverage in 24 hours and provide SV detection down to 5% VAF. Further extend runs to see even lower VAF. Experience seamless integration and workflow using the Saphyr™ Chip® consumable and Saphyr™ system. The Saphyr Chips utilize hundreds of thousands of massively parallel nanochannels that linearize long, labeled DNA molecules, allowing the Saphyr instrument to directly image your samples. Sample loading on the Saphyr Chip is fast and easy. Pipet up to three samples into individual flow cells on the chip. Load up to two chips onto the Saphyr instrument per run for a maximum throughput of six samples per run. The Saphyr instrument and chips leverage machine learning-based adaptive loading and automatic run optimization to ensure walk-away scanning and the fastest possible throughput for runs. The Saphyr™ Chip® consumable: The Saphyr Chips utilize hundreds of thousands of massively parallel nanochannels that linearize long, labeled DNA molecules, allowing the Saphyr instrument to directly image your samples. Sample loading on the Saphyr Chip is fast and easy. Pipet up to three samples into individual flow cells on the chip. Load up to two chips onto the Saphyr instrument per run for a maximum throughput of six samples per run.
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Simple, Automated Genomic DNA and RNA Isolation Downstream analytical assays require the highest level of yield and purity to ensure the best representation of your sample. Bionano’s Nucleic Acid Purification Kits provide a simple and efficient method for extracting DNA or RNA from even the most precious and challenging samples. Once the desired sample has been prepped using the corresponding Nucleic Acid Purification Kit, simply load the sample onto the Ionic. Ionic® Purification Kits for FFPE and Related Samples Bionano provides a suite of nucleic acid purification sample prep kits for simple and automated isolation of genomic DNA and RNA from a range of valuable sample types. Once the desired nucleic acid has been isolated, you can go straight into the next step of your workflow. Achieve nucleic acid extraction from valuable and low- yielding samples Using the Ionic purification kits, you can achieve a high level of isolation and even sample recovery, with traditionally challenging samples. Using 50 – 300 mm 2 cross sections of sample, successfully isolate the desired nucleic acid in approximately 75 minutes with minimal hands-on time. Maximize purification yield and quality Using the Ionic purification kits, recover roughly 4.5x more DNA from FFPE sample over traditional column methods. Bionano’s Ionic purification kits with G2 chemistry produce greater purity and concentration than leading isolation methods for increased confidence in your assays. Streamline your workflow by utilizing the power of automation Using the Ionic system with Bionano’s Ionic purification kits to enhance throughput, a single operator can isolate nucleic acid from up to 48 samples a day. This system requires just a few minutes of hands-on time per sample, minimizing user error and improving reliability. The Ionic system simplifies and accelerates nucleic acid purification, resulting in higher yields of higher quality DNA. Increase Efficiency with a Simplified FFPE Workflow 3 Minutes Hands-on Time per Chip with Fewer Manual Steps The Ionic®Purification System builds efficiency in your experimental workflow in addition to the advantages in quantity and quality of nucleic acids extracted. Extract eight samples with minimal hands-on time and no separate steps required to de-paraffinize or de-crosslink samples.
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The Bionano solution phase (SP) technology allows for the isolation of DNA fragments that can achieve 1 Mbp in length and routinely yields average fragments of 230 kbp. Putting this in perspective, short read sequencing produces reads of up to 150 bp while leading high accuracy long-read sequencing provides read lengths up to 25kb. The latest Bionano SP DNA prep kits are capable of purifying UHMW DNA from tissue and tumor, bone marrow aspirate (BMA), blood and cells as well as plant and animal tissue. This sample diversity makes OGM suitable for use in a broad range of studies and applications in oncology, constitutional genetic disease, bioprocessing and general research. Bionano’s SP kits require 1.5 10^6 cells (blood, cell lines and BMA) or 10 – 30 mg of tissue as input. UHMW DNA is isolated in about four hours using a lyse, bind, and wash process, and novel paramagnetic disks. Our Generation 2 Kits, with robust enhancements and optimizations to our sample preparation OGM workflow allows you to achieve sample-to-answer in just 3 to 4 days!
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