LIMA™ Hyperspectral Microscope with Tunable Excitation

By Photon etc

LIMA™ is a hyperspectral microscope based on a widely tunable laser source. It uses Photon etc.'s filtering technology to illuminate the full field-of-view of a research-grade microscope with a continuously tunable monochromatic, high power density laser light. The system provides a high spectral resolution in the VIS, NIR, and SWIR combined to a near-diffraction-limited spatial resolution. Ideal for darkfield, PLE or standard brightfield reflectance and transmittance imaging, it can map the full spectral response of a sample much faster than point-by-point or line-scan-based systems. TECHNICAL SPECIFICATIONS VIS - SWIR MODEL eSWIR 400 - 1620 nm VIS SWIR Excitation spectral range 400 - 1000 nm 900 - 1620 nm 1000 - 2500 nm Excitation spectral resolution (FWHM) 1.5 - 2.5 nm 3.0 - 5.0 nm < 5.0 nm Excitation wavelength Continuously tunable Out-of-band rejection < -60dB @ ±40nm < -60dB @ ±80nm < -60dB @ ±80nm TLS output power 2 to 4 mW 3 to 9 mW 0.5 to 8 mW Illumination High efficiency homogeneous illumination Dia or Epi illumination; Brightfield; Darkfield (Oil and Dry) Spatial resolution Sub-micron - limited by the microscope objective NA Camera sCMOS (optionally CCD, EMCCD) Photon etc. InGaAs camera (ZephIR™ 1.7 or Alizé™ 1.7) Photon etc. HgCdTe camera (ZephIR™ 2.5) Microscope Upright or inverted, scientific grade Wavelength absolute accuracy FWHM/8 Scanning speed 20 to 50ms stabilization time (for step sizes 0.01 to 10 nm) X, Y travel range 76 mm x 52 mm (with a manual stage) Z stage resolution 100 nm White light illumination Diascopic, episcopic, Hg, halogen Preprocessing Spatial filtering, statistical tools, spectrum extraction, data normalization, spectral calibration, overlay, central position map, etc. Hyperspectral data format HDF5, FITS Software PC (Windows10 - 64-bits) with PHySpec™ control and analysis software (computer included) Dimensions Microscope: 31 cm x 85 cm x 82 cm Supercontinuum laser: Up to 44 cm x 25.1 cm x 40 cm Tunable filter: Up to 30 cm x 23 cm x17.5 cm Combined: Less than 105 cm x 85 cm x 82 cm Weight ≈ 80 Kg (Gross 220 lbs; Net 180 lbs) Power requirement 120 VAC / 12 A / 60 Hz 230 VAC / 12 A / 50 Hz OPTIONS & ACCESSORIES Objectives magnification: 20X, 40X, 50X, 60X, 100X (5X and 10X available on demand) Motorized stage: 100 mm x 100 mm travel, 22 nm resolution Filter wheel: up to 6 band-pass filters Second camera port High resolution module: VIS < 0.5nm; SWIR < 1.0nm Hyperspectral filter in detection (2D spectroscopic micro-imaging) Polarization control (polarizer & analyser) Optical table with passive anti-vibration isolation recommended: 900 x 1800 x 60 mm (36 x 72 x 2.4 inches) or 900 x 900 x 60 mm (36 x 36 x 2.4 inches) next to 900 x 900 mm (36 x 36 inches) standard table DARKFIELD HYPERSPECTRAL MICROSCOPY Not only the LIMA can rapidly localize nanoparticles in a sample but the acquisition of the scattering spectrum offers much more insight into the nature of the sample. For example, the intensity and position of the scattering peak help to identify the size and shape of each nanoparticle. PHOTOLUMINESCENCE EXCITATION AND REFLECTANCE MAPPING Photoluminescence Excitation (PLE) and Reflectance hyperspectral data of perovskite films acquired over the same field of view. The combination of those optical measurements can guide fabrication methods by helping the identification of defects, inhomogeneities, and losses. APPLICATIONS Darkfield hyperspectral image of 100 nm gold nanoparticles using a 40X dry objective TUNABLE LASER OUTPUT POWER Reflectance Integrated PL (750 to 1000nm) with 550 nm excitation *PL option available on demand -hyperspectral image not shown here. With NKT Photonics SuperK FIU-15 With NKT Photonics SuperK FIR-20