By MMI Molecular Machines and Industries AG
Isolation petri dishes (ø 35 mm) with a silicone bottom 10 dishes per pack The MMI Isolation Dishes in combination with the MMI CellCut and MMI Membrane Rings enable contamination-free isolation of living cells in culture. Enzyme treatments (i.e. trypsin), other potentially harmful selective reagents, and time-consuming repetitive enrichments can be avoided. The benefit is an increase in reliability, effectiveness and accuracy of your research. - Gentle living microdissection - Living cell cutting without removing culture media - Highest cell integrity for meaningful -omics applications and re-cultivation.
The MMI CellScan is the most flexible whole slide imaging system on the market. With compatibility across a wide range of research microscopes and any possible imaging mode, CellScan maximizes the performance of your research or pathology services. CellScan is a whole slide scanner that converts any sample format into high-resolution digital data in 5D: Time-lapse, Z-stack, XY Scan, and multi-color fluorescence for the deepest insights into your sample. CellScan is not just a whole slide scanner; it’s a comprehensive imaging solution tailored to meet the demands of modern research. Elevate your imaging experience with CellScan’s advanced features and take your research to new heights. Whole slide scanner with the highest flexibility CellScan is compatible with all objectives, numerous research microscopes and all possible imaging modes. Together with the MMI CellCut, CellEctor and CellDetector it enables new areas of applications, like single cell isolation and automated cell analysis. High-performance whole slide scanner The entire imaging process is in your control, allowing you to scan a 15 mm x 15 mm section in less than 60 seconds and a full 75 mm x 25 mm slide in less than 7 minutes (20x objective). Seamlessly operate from any location Unlock the potential of virtual microscopy and view your whole slide images remotely with unlimited licenses of our MMI imaging software. Your digital images and slides can be stored, viewed, shared, and accessed globally. Collaborate with colleagues by sharing and virtually discussing your results. Scan your whole slides in 5 dimensions Cell Scan enables the whole slide scanning of full-resolution digital slides in 5D: XY-Scan, Z-stacks, time-lapse and multi-fluorescence, without losing quality. Obtain high-resolution images to fulfil the standards for impactful publications. Whole slide imaging meets laser microdissection Combine your whole slide scanner with the CellCut laser microdissection system. Benefit from a seamless transition between scanning and microdissection for an efficient workflow. User-friendly operation For technical assistants, the process is user-friendly — simply place your slides under the microscope and activate the device. The open x-y stage allows easy access to up to four standard slides, making slide changes and cleaning straightforward. Digital microscopy was never so easy. Compatible with almost all microscopes and imaging modes The MMI CellScan is compatible with almost all research microscopes. You decide what microscope to use. Therefore, CellScan is not just a whole slide scanner, it’s a gateway to limitless digital imaging possibilities. Whether you’re working with brightfield, multi-fluorescence, confocal, phase contrast, or any other imaging mode, this versatile whole slide scanner gives you the freedom to explore and capture your samples in unprecedented detail. All common sample formats The whole slide scanner CellScan supports various sample formats. From conventional glass slides (including coverslips) to petri dishes with living cells, and membrane slides designed for laser microdissection. This unmatched flexibility ensures that CellScan is tailored to meet the diverse needs of various applications, like in digital pathology, cell biology and oncology. 5D imaging Take control of your research with CellScan’s ability to scan full-resolution digital slides in 5D. Capture dynamic changes over time with time-lapse imaging, explore the depths of your samples in Z-stack mode, navigate seamlessly in the x-y axis, and unravel the complexity of multi-fluorescence imaging. The MMI whole slide scanner empowers you to conduct experiments and analyse exactly as you envision. Modularity is key The whole slide scanner CellScan boasts exceptional modularity, offering seamless compatibility and upgradability with all MMI devices. With modules like CellCut for precise laser microdissection, CellEctor for isolating cells in suspensions, and CellDetector utilizing artificial intelligence for automatic cell and tissue identification, our whole slide scanner provides a versatile platform for evolving research needs. It’s a whole slide scanner - Full resolution in any magnification - Any sample type - In 5D (x, y, z, time, colour) - Open file format BigTIFF It’s a microscope - Anything you would do with a microscope (e.g. any objective lenses and fluorescence filter) - Any image mode, including confocal Its upgradeable - With various microscope accessories - With any other MMI system (laser microdissection, cell picking and automated cell/tissue detection)
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LCM is the powerful technique used to isolate single cells or a targeted cell group with extreme microscopic precision. This technology can selectively isolate single cells from various origins, such as from tissue sections, cytospins or smears, and from cell culture to perform single cell omics.LCM focuses on using a low-pulse ultraviolet laser on membrane-mounted sections of tissue. The membrane offers good optical quality without interfering with staining and the downstream analysis. What makes LCM the gold standard for cell isolation? The combination of the MMI CellCut LCM system with the ZEISS Axio Observer inverted microscope allows for the precise and gentle isolation of single cells without compromising the spatial information of the cell’s micro environment. 1. The most gentle isolation LCM with the MMI CellCut and ZEISS Axio Observer features the cleanest laser on the market for dissection. The specialized laser creates an exceptionally precise cutting edge as thin as 0.3 µm, protecting the surrounding area and preserving biological information. Additionally, the sample stage moves, keeping the laser beam consistently in focus and maintaining a minimal cutting edge. This makes the MMI CellCut the most delicate method for cell isolation. 2. Check your efficiency with 100% success rate The unique CapLift technology ensures nearly a 100% success rate in cell isolation. This technology uses an Isolation Cap with an adhesive surface that captures the sample after dissection, allowing for visual inspection of the excised sample. 3. Contamination-Free Isolation During the cutting process, cell isolation is achieved without direct contact between the isolation cap and the sample. This is done using a sandwich technique, where the sample is placed between a membrane slide and a glass slide, ensuring the process remains entirely contamination-free. 4. High-Throughput Automation The LCM system from MMI and ZEISS can be enhanced with an automated, high-throughput guided workflow for cell isolation. The innovative CellRobot system enables the dissection of samples in a 96-well plate in less than 10 minutes. 5. Cut it all The cell isolation system is versatile to dissect almost any type of sample. It can handle fresh frozen tissue, FFPE material, plant tissue, living cells, smears, cytospins, and more. It can also cut through thick tissues such as teeth, bones, or forensic tapes. Even living cells can be isolated without removing the culture medium, preserving cell integrity for subsequent recultivation, cloning, or single cell analysis. The integration of a climate chamber with full environmental control is also possible. 6. Become an expert in 10 minutes The system is incredibly user-friendly, allowing you to become a professional in just 10 minutes. The intuitive software provides a user-friendly interface, enabling detailed documentation of every cell isolation step (including images and videos). 7. Unmatched Flexibility The LCM CellCut system is the most flexible option for cell isolation. It supports all standard objectives and imaging modes. Additionally, the system can be upgraded with a whole slide scanner, a cell picker, and AI for detecting specific cells. 8. Exceptional Imaging Quality with the ZEISS Axio Observer Paired with the ZEISS Axio Observer, this system delivers exceptional performance with flexibility to support future growth. The ZEISS Axio Observer provides exceptional optical performance, superior resolution, and advanced imaging capabilities, ensuring that even the most delicate samples are captured with extraordinary detail.
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The MMI CellCut facilitates precise and contamination-free dissection of cell clusters, single cells or subcellular compartments from various types of tissues including fresh frozen, paraffin-embedded and archived slides, cytospins, smears and even living cells. Gentle to Cells: CellCut ensures the highest sample integrity by airtight and sterile cutting and collection. This will make your downstream applications more efficient to achieve reproducible results. Cut it Clean: CellCut has the most precise cutting performance on the market. This ensures the thinnest and cleanest cut. Your sample will be not degraded. Keep it Flexible: CellCut is the most flexible laser microdissection system. Compatible with many microscopes and almost all objective lenses. Update your laser microdissection system to produce Whole Slide Images with CellScan. Check your Efficiency: In contrast to systems that use gravity for collection, CellCut enables a visual inspection and documentation of cut samples. Always check your cutting efficiency. Cut it All: You can cut almost any type of sample, such as fresh frozen tissue, FFPE material, plant tissue, smears, cytospins and living cells. Even the cutting of large areas beyond the field of vision is no problem. Cut them Alive: Dissect living cells without removing culture media to ensure highest cell integrity for re-cultivation, cloning and single cell analysis. With over 20 years of experience in laser microdissection, we at Molecular Machines & Industries make the difference. Our team of passionate researchers have developed the most precise laser microdissection system on the market. The gentle cutting guarantees a homogeneous cell population for meaningful downstream analysis. The contamination-free cutting with full visual inspection at any step during and after isolation enables outstanding single cell cutting performance. The MMI CellCut is highly modular and can be mounted on numerous brands of microscopes, from entry-level, mid-range to high-end instrumentation, suitable for routine applications as well as most complex research projects. The most gentle microdissection: Isolate your sample without any degradation and ensure meaningful downstream analyses in genomics, transcriptomics, proteomics, lipidomics and metabolomics. The cleanest laser microdissection on the market is enabled by the maintenance-free MMI laser. The low pulse energy laser (less than 500 ps) creates a very precise cutting edge (up to 0.3 µm) and therefore protects the surrounding area and biological information (RNA, DNA, Proteins). Move the stage and your laser is always in focus: By moving the stage and not the laser we ensure that the laser beam is always in focus. Other laser microdissection systems which move the laser can only cut samples in the field of view – leading to inaccurate cutting results. - Most accurate and precise cutting system on the market - No sample degradation for meaningful downstream applications Where is my sample? The MMI CellCut laser microdissection allows a full visual inspection of your sample during and after isolation. The unique MMI CapLift technology makes this possible. The CapLift technology uses an adhesive surface that keeps the sample in place during cutting. The orientation of the cell will be maintained, which allows you to easily verify the presence of the dissected area in the Isolation Cap. Furthermore, you can keep your sample in place during cutting to prevent single cells from flipping away. You will love this feature for your work on rare cells, and so will your reviewer! - Always check your cutting efficiency - Gentle, contamination-free and highly efficient sample collection with MMI CapLift technology - Gravity is not used for collection (Gravity allows no sample inspection and can damage the collected sample) Microdissection for almost any type of sample: With the MMI CellCut, you can dissect fresh frozen tissue, FFPE material, plant tissue, living cells, smears, cytospins and more. Thick tissue is no problem: Do you need to cut thick or wet tissue? No problem! The “Z-drill feature” allows to refocus the laser on different z-levels. Therefore, you minimise the use of excessive laser power and so preserve your sample and maximize its usability for downstream analysis. An alternative high-power laser is available to cut very hard materials such as teeth, bones or forensic tapes. Size does not matter: Dissect single cells or huge tissue areas – even beyond the field of vision. The precise laser is fixed while the stage is moving during laser cutting, thus the laser will always be in perfect focus, even with larger tissue areas. Enhancing precision in sample collection: The MMI laser microdissection system used the patented CapSure technology for the precise and contamination-free isolation of specific cells or regions of interest from tissue samples. The CapSure technology used a small adhesive cap, which automated the transfer of the target sample from the slide into a microcentrifuge tube (Isolation Cap). The Isolation Cap only gets into contact with the membrane – the sample itself is not touched. Therefore the sample can be handled free of contamination. The gentle cutting and the transfer without unnecessary induction of physical or chemical forces leads to meaningful results for any downstream applications. - With CapSure you can always check your cutting efficiency - Contamination-free cutting - Gentle transfer of cut samples to ensure meaningful downstream applications Isolation of single cells with CellCut: The isolation with the “sticky caps” enables the collection of single cells, cell clusters, and subcellular components with nearly 100% efficiency, as demonstrated by independent studies. They feature a small adhesive area filled with inert silicone, allowing for seamless transfer of target samples from the Membrane Slide to the isolation tube.” Why is high collection efficiency important? In spatial omics research, high collection efficiency is essential for reliably transferring defined tissue regions. This is especially critical for maintaining data quality in automated workflows and when working with limited sample material. Predefined Target Positioning: The Predefined Target Positioning (PTP) feature allows a contamination-free and controlled collection of individual cells on the Isolation Cap. PTP is excellent to maximize the number of samples collected into a single reaction tube, guarantees isolated samples are not compromised by subsequent cutting and is essential for auditability. MMI MultiCap and MultiSlide: The MMI MultiSlide enables you to process multiple samples in a single run, saving time and increasing efficiency in the laser microdissection workflow. You can isolate single cells into up to eight individual caps without the need to reload. The MultiSlide can also be used to analyze multiple regions within a single sample, allowing for a more comprehensive analysis of tissue or cell heterogeneity. Live cell cutting without removing culture media: Isolate living cells to obtain a homogeneous live-cell population. In contrast to systems, that use upright microscopes, our laser comes from below. Therefore, you can dissect living cells without removing culture media to ensure highest cell integrity for meaningful -omics applications and re-cultivation. Attach a climate chamber: You can equip the MMI CellCut with a climate chamber with full environmental control - Gentle living microdissection for meaningful downstream applications - Attach a climate chamber to the LMD system - Grow living cells on petri dishes with PEN membrane or multi-well ibidi slide MMI CellTools: The user-friendly solution for your microdissection: With CellCut you will be familiar within minutes. The intuitive CellTools software provides a user-friendly interface for controlling the microscope and the microdissection instrument for capturing cells of interest. Additionally, CellTools offer tools for annotating and labelling regions of interest, as well as for tracking and managing captured cells. Cutting cells was never so easy. - Control laser and microscope - Select regions for cutting by mouse or pen screen - Fully auto documentation for every cutting step (images & videos) - Import your ROIs from other devices (e.g. slide scanners) Customize your microdissection: Customization is key for your research! The MMI CellCut is compatible with almost all microscopes and objective lenses – perfect for unique applications. You can integrate our laser microdissection system on a confocal microscope, or you can combine it with Raman spectroscopy. For live cell applications, you can also integrate the system into an incubation hood. Furthermore, the CellCut is compatible with all MMI single cell solutions (MMI CellScan, MMI CellEctor, MMI CellDetector and MMI CellManipulator) - You can use almost all inverted/confocal microscopes and objective lenses - Compatible with all other MMI Single Cell solutions Whole slide imaging and beyond: Laser microdissections with the MMI CellCut can easily be combined with the MMI CellScan – a whole slide scanner to scan full-resolution digital slides. Combining laser microdissection with whole slide imaging (WSI) allows researchers to image a tissue section and then precisely select cells and dissect the tissue while concurrently preserving information about the original uncut tissue and position information about the cut. Thus, laser microdissection will be easily integrated into your digital pathology workflows. - Improve efficiency in pathology diagnostics and research by combining microdissection with whole slide imaging - Reduces hands-on time at the instrument by analysing your digital slides remotely
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Cell Picking with the MMI CellEctor is crucial when you need to isolate individual cells (e.g. circulating tumor cells, rare cells, sperm cells etc.) for further analysis or experimentation. The open cell picker platform allows the integration of your own imaging, detection and picking protocol with automated isolation and recognition and is therefore fundamental for many research projects in pathology, oncology and forensics. What are the benefits of the MMI cell picking system? When every cell counts The MMI CellEctor cell picker is crucial when precise examination of individual cells is required, even in cases of limited cell quantities. The cell picker is essential in forensics or working with rare cells like circulating tumor cells (CTCs). No contamination from unwanted cells. Automated cell detection and picking Use the optional MMI CellDetector AI analysis software to detect and isolate fully automated rare cells based on their color, morphology and relative size. Unmatched precision High-precision microfluidic cell picking robot for gentle cell transfer for the highest cell integrity and growth rates after cell transfer. Up to 100% picking/transfer efficiency of living or fixed cells. User-defined liquid handling Full optical control of your own workflow for automated collection and deposition of single cells in suspensions. The cell picker is compatible with a wide range of slide-based platforms including PCR tubes, IBIDI and different well plates (e.g. 96-well plate). Isolation of single cells like a pro With the intuitive and flexible MMI CellTools analysis software you achieve accurate and precise cell isolation and depositions. Cell picking has never been easier. The most flexible cell picking system The CellEctor cell picker is compatible with most inverted microscopes and fully expandable with all other MMI solutions: Laser microdissection (CellCut), whole slide imaging (CellScan) and optical trapping (CellManipulator). The MMI CellEctor cell picking system automated the cell pickup process using a microscope-based micro-capillary sorting system. The highly precise MMI CellPump enables single cell isolation even in nanoliter volumes. Since the system is fully motorized and computer controlled, it offers complete control in manual and automated cell recognition, acquisition and deposition modes. The MMI CellEctor allows researchers to isolate and manipulate desired cells without the need for complex sorting techniques like Fluorescence-Activated Cell Sorting (FACS). While FACS is efficient for high-throughput sorting based on specific markers, it may not be suitable for certain applications where the target cells are rare or require more precise selection. In such cases, cell picking with the MMI CellEctor provides a viable alternative, enabling researchers to manually choose and collect individual cells of interest. Cell picking with the MMI CellEctor enables extremely gentle cell transfer for the highest cell integrity and outgrowth rates after cell transfer The cell picker is Ideal for isolation in cases of limited cell quantities Cell picking with the MMI CellEctor gives the user complete control in manual and automated cell identification, acquisition and deposition. The cell picking process is contamination-free and supports a broad range of sample types. The high flexibility of the cell picker enables you to define your own workflow. Just select the wells for cell picking, deposition and rinsing using a wide range of slide-based platforms. With the ground-breaking shake mode feature, the MMI CellEctor cell picker can isolate adherent cells without compromising their viability or integrity. Thus, living adherent cells can now be selectively isolated and utilized for any downstream applications, even for sensitive RNA expression analysis.
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