The Automated Cell Monitoring System cellZscope systems measure the transepithelial / -endothelial impedance of cell layers under physiological conditions. They are computer-controlled and allow automated, long-term monitoring experiments with up to 96 different cell cultures simultaneously. Various types of barrier-forming cells cultured on permeable membranes can be analyzed. The ohmic resistance (TER, transepithelial / -endothelial resistance) and optionally the capacitance (Ccl) of the cell layers under investigation are determined in real-time. The cellZscope is easy to operate and has a broad range of possible applications. In particular, it is ideally suited for studying the influence of substances such as drugs, toxins etc. on the barrier function of cell layers. As the original innovator of label-free automated monitoring of barrier forming cells grown in standard cell culture inserts, nanoAnalytics designed the cellZscope systems with highest precision engineering and quality materials. Features are a convincing performance, great measurement flexibility, and instrument operations and maintenance that are truly a snap. These and other features have made the cellZscope systems the ideal tool for controlling cellular barriers. The real strength of the cellZscope lies in its capability to sweep fast and accurately over a wide frequency range instead of measuring at just a few frequency points. Only the full spectral information detected by the cellZscope ensures a reliable and unambiguous detection of cell layer properties and changes thereof. Maximum flexibility – don't stick to one size Depending on the model, cellZscope systems can be loaded with up to 96 inserts simultaneously. Three well sizes are available for use with small (24-well type), mid-sized (12-well type), or large (6-well type) inserts, respectively. The Cell Module features individual (stand-alone) stainless steel wells. This also allows the user to combine different well sizes within one Cell Module, measuring simultaneously and comparing cell layers grown on small, mid-size, or large inserts in a single experiment. With its innovative design the cellZscope offers full compatibility with standard cell culture inserts from all major manufacturers, like Corning Transwell / Falcon, Greiner Bio-One ThinCert, Sarstedt and Millipore Millicell. During an experiment the Cell Module is typically placed inside a standard incubator while the external Controller is connected to a computer running the cellZscope software. Easy to clean – sustainable and effortless The electrode design makes cleaning very easy. Single-piece stainless steel 'pots' serve as bottom electrodes, as vessels for holding the cell culture medium, and as supports for cell culture inserts. They can be detached easily without the use of tools. The innovative design of the lid stands out with its high user-friendliness and makes handling of the upper electrodes comfortable. All stainless steel electrodes and all parts of the Cell Module lid are autoclavable. These features make the cellZscope systems a snap to clean and sterilize. No additional device-specific disposables are required, keeping customer operating costs at a minimum. Software – intuitive and clear layout The cellZscope software features advanced multi-channel data acquisition with data processing. The software is highly intuitive providing the user complete control of the experiment. TER and capacitance of the cell layer are displayed in real time. Other features of the cellZscope software include visualization of raw data acquisition, statistical analysis, and comprehensive documentation of experimental parameters and events. The measurements can be scheduled by specifying time intervals according to experimental requirements. User-friendly functions for exporting cellZscope data into popular file formats are also included. Setting up and controlling the experiment is easy and intuitive. Barrier property dynamics can be visualized in real-time. Powerful offline data processing and easy export of graphs, results and raw data are supported. The crucial parts are designed using the highest quality stainless steel – ensuring long-lasting durability and excellent compatibility with all standard cleaning and sterilization methods. cellZscope systems are designed to ensure simple operation and maximum flexibility. The Cell Module provides investigators full apical and basolateral access to all wells. 24 individual wells, measurement of TER and Ccl every 5 minutes [*]. Exceptional ease of use: handling during experiments is a snap with the Docking Station. 24 individual wells, measurement of TER and Ccl every 20 minutes [*]. Sustainable, easy to use and maintenance without the use of tools. 6 individual wells, measurement of TER every hour. Entry-level model that goes easy on the budget. Later upgrade to a full cellZscope® possible. High-end model with up to 96 individual wells per Controller [*], measurement of TER and Ccl. Top performance: standard time resolution of 30 seconds [*] with full spectral information.
24-Well Inserts (PET membrane, individually packaged, box with 48 pcs.) - 932 04 02: translucent, 0.4 µm, 1×10 8 /cm 2 - 932 04 12: clear, 0.4 µm, 2×10 6 /cm 2 - 932 10 02: translucent, 1.0 µm, 2×10 6 /cm 2 - 932 10 12: clear, 1.0 µm, 2×10 6 /cm 2 - 932 30 02: translucent, 3.0 µm, 2×10 6 /cm 2 - 932 30 12: clear, 3.0 µm, 0.6×10 6 /cm 2 - 932 50 02: translucent, 5.0 µm, 0.6×10 6 /cm 2 - 932 50 12: clear, 5.0 µm, 0.6×10 6 /cm 2 - 932 80 02: translucent, 8.0 µm, 0.2×10 6 /cm 2 - 932 80 12: clear, 8.0 µm, 0.1×10 6 /cm 2
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Stick to your favourite cell culture insert! cellZscope TEER measurment systems are compatible with a variety of standard cell culture inserts from different manufacturers. Three versions of the electrodes are available. They are tailored for use with small (“24-well” type), mid-sized (“12-well” type), or large (“6-well” type) inserts, respectively. Different well sizes can be combined within one Cell Module. The following table displays a selection of cell culture inserts compatible 1 with the cellZscope. Feel free to reach out to us with any questions. Manufacturer Item number Membrane Insert type 24 well size 12 well size 6 well size Material Pore size Pore density Optics Corning Transwell 3413 3410 3412 PC 0.4 µm 1.0x10 8 /cm 2 translucent hanging 3470 3460 3450 PET 0.4 µm 4.0x10 6 /cm 2 clear hanging 3415 3402 3414 PC 3.0 µm 2.0x10 6 /cm 2 translucent hanging 3472 3462 3452 PET 3.0 µm 2.0x10 6 /cm 2 clear hanging Corning Falcon 353495 353494 353493 PET 0.4 µm 1.6x10 8 /cm 2 translucent hanging 353405 353180 353090 PET 0.4 µm 1.6x10 6 /cm 2 clear hanging 353104 353103 353102 PET 1.0 µm 1.6x10 6 /cm 2 clear hanging 353492 353292 353092 PET 3.0 µm 2.0x10 6 /cm 2 translucent hanging 353096 353181 353091 PET 3.0 µm 8.0x10 5 /cm 2 clear hanging Greiner Bio-One ThinCert 662 640 665 640 657 640 PET 0.4 µm 1.0x10 8 /cm 2 translucent hanging 662 641 665 641 657 641 PET 0.4 µm 2.0x10 6 /cm 2 clear hanging 662 610 665 610 667 610 PET 1.0 µm 2.0x10 6 /cm 2 clear hanging 662 631 665 631 667 631 PET 3.0 µm 2.0x10 6 /cm 2 translucent hanging 662 630 665 630 667 630 PET 3.0 µm 6.0x10 5 /cm 2 clear hanging Merck Millipore Millicell MCHT 24H 48 MCHT 12H 48 MCHT 06H 48 PET 0.4 µm 1.0x10 8 /cm 2 translucent hanging MCRP 24H 48 MCRP 12H 48 MCRP 06H 48 PET 1.0 µm 2.0x10 6 /cm 2 clear hanging MCSP 24H 48 MCSP 12H 48 MCSP 06H 48 PET 3.0 µm 2.0x10 6 /cm 2 translucent hanging Brand 7827 00 7827 20 7827 40 PS 0.4 µm 1.0x10 8 /cm 2 translucent standing Nunc 140620 140652 140660 PC 0.4 µm 8.5x10 7 /cm 2 translucent standing 140627 140654 140663 PC 3.0 µm 1.7x10 7 /cm 2 translucent standing Sarstedt 83.3932.040 83.3931.040 83.3930.040 PET 0.4 µm 1.0x10 8 /cm 2 translucent hanging 83.3932.041 83.3931.041 83.3930.041 PET 0.4 µm 2.0x10 6 /cm 2 clear hanging 83.3932.101 83.3931.101 83.3930.101 PET 1.0 µm 2.0x10 6 /cm 2 clear hanging 83.3932.300 83.3931.300 83.3930.300 PET 3.0 µm 2.0x10 6 /cm 2 translucent hanging SABEU cellQART 932 04 02 931 04 02 930 04 02 PET 0.4 µm 1.0x10 8 /cm 2 translucent hanging 932 10 02 931 10 02 930 10 02 PET 1.0 µm 2.0x10 6 /cm 2 translucent hanging 932 30 02 931 30 02 930 30 02 PET 3.0 µm 2.0x10 6 /cm 2 translucent hanging 932 04 12 931 04 12 930 04 12 PET 0.4 µm 2.0x10 6 /cm 2 clear hanging 932 10 12 931 10 12 930 10 12 PET 1.0 µm 2.0x10 6 /cm 2 clear hanging 932 30 12 931 30 12 930 30 12 PET 3.0 µm 6.0x10 5 /cm 2 clear hanging Kanto Chemical ad-MED Vitrigel 2 08364-96 08363-96 - Collagen - - clear hanging CELLTREAT 230629 230615 230601 PC 0.4 µm hanging 230635 230621 230607 PET 0.4 µm hanging 230631 230617 230603 PC 3.0 µm hanging 230637 230623 230609 PET 3.0 µm hanging If you are using one of our devices, the recommended cell culture medium volumes are provided below. The specified volumes correspond to a fill level that ensures the upper electrodes are submerged 2 mm in the apical medium. Manufacturer Item numbers Insert size Medium volume Apical Basolateral Testing Corning Transwell 3413, 3470, 3415, 3472 24 well 0.25 ml 0.99 ml 1.80 ml 3410, 3460, 3402, 3462 12 well 0.83 ml 1.48 ml 3.50 ml 3412, 3450, 3414, 3452 6 well 3.63 ml 4.38 ml 10.00 ml Corning Falcon 353495, 353405, 353104, 353492, 353096 24 well 0.40 ml 0.77 ml 1.80 ml 353494, 353180, 353103 353292, 353181 12 well 0.81 ml 1.46 ml 3.50 ml 353493, 353090, 353102 353092, 353091 6 well 3.97 ml 3.96 ml 10.00 ml Greiner Bio-One ThinCert 662 640, 662 641, 662 610, 662 631, 662 630 24 well 0.24 ml 0.98 ml 1.80 ml 665 640, 665 641, 665 610 665 631, 665 630 12 well 0.65 ml 1.66 ml 3.50 ml 657 640, 657 641, 667 610 667 631, 667 630 6 well 2.97 ml 5.05 ml 10.00 ml Merck Millipore Millicell MCHT 24H 48, MCRP 24H 48, MCSP 24H 48 24 well 0.28 ml 0.93 ml 1.80 ml MCHT 12H 48, MCRP 12H 48 MCSP 12H 48 12 well 0.62 ml 1.54 ml 3.50 ml MCHT 06H 48, MCRP 06H 48 MCSP 06H 48 6 well 3.02 ml 4.78 ml 10.00 ml Brand 7827 00 24 well 0.44 ml 0.68 ml 1.80 ml 7827 20 12 well 0.97 ml 1.23 ml 3.50 ml 7827 40 6 well 3.85 ml 3.81 ml 10.00 ml Nunc 140620, 140627 24 well 0.44 ml 0.68 ml 1.80 ml 140652, 140654 12 well 1.09 ml 1.11 ml 3.50 ml 140660, 140663 6 well 3.36 ml 4.63 ml 10.00 ml Sarstedt 83.3932.040, 83.3932.041 83.3932.101, 83.3932.300 24 well 0.24 ml 0.98 ml 1.80 ml 83.3931.040, 83.3931.041 83.3931.101, 83.3931.300 12 well 0.65 ml 1.66 ml 3.50 ml 83.3930.040, 83.3930.041 83.3930.101, 83.3930.300 6 well 2.97 ml 5.05 ml 10.00 ml SABEU cellQART 932 04 02, 932 10 02, 932 30 02 932 04 12, 932 10 12, 932 30 12 24 well 0.23 ml 0.99 ml 1.80 ml 931 04 02, 931 10 02, 931 30 02 931 04 12, 931 10 12, 931 30 12 12 well 0.66 ml 1.61 ml 3.50 ml 930 04 02, 930 10 02, 930 30 02 930 04 12, 930 10 12, 930 30 12 6 well 3.10 ml 4.87 ml 10.00 ml Kanto Chemical ad-MED Vitrigel 2 08364-96 24 well 0.17 ml 1.05 ml 1.80 ml 08363-96 12 well 0.52 ml 1.77 ml 3.50 ml CELLTREAT 230629, 230635 230631, 230637 24 well 0.20 ml 1.03 ml 1.80 ml 230615, 230621 230617, 230623 12 well 0.65 ml 1.65 ml 3.50 ml 230601, 230607 230603, 230609 6 well 3.37 ml 4.17 ml 10.00 ml made by SABEU cellQART cell culture inserts in 6-, 12-, and 24-well format are 100% compatible with all cellZscope systems.
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Full control of experiment and results with the cellZscope software! The cellZscope comes with customized software which provides full instrument control including user defined experiment setup and automated data acquisition. Furthermore, it features comprehensive tools for displaying and statistically analyzing data as well as printing and exporting results. The user interface is geared to easy operation of the instrument. Main features are: - Schedule measurements by specifying time intervals for data acquisition according to experimental requirements, - monitor results in real time, - enter individual well descriptions and notes, - log and annotate all experimental events. The cellZscope software allows to monitor and record all relevant parameters during the course of the experiment, in particular it provides the - transepithelial endothelial electric resistance TEER and the - electric capacitance C cl of the cell layer as easy readout parameters. User-friendly functions for exporting cellZscope data into popular file formats are included, making seminar and manuscript preparations a breeze.
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