By Protochips
In Situ Gas Cell Study atomic-scale gas-solid interactions in the (scanning) transmission electron microscope to drive innovation in heterogeneous catalysis, materials science, and more. Atmosphere AX is an in situ gas phase system for transmission electron microscopy (TEM). It enables researchers to observe their materials under realistic, functional conditions while taking advantage of the resolving power of the TEM. With full control over temperature, pressure, and gas/humidity composition, it allows for direct visualization of processes like catalytic activation, operation, and degradation. Flexible in situ environments: Full control to create the most relevant environments with unmatched flexibility in gas mixing and compatibility Machine vision software suite: Integrated machine vision software manages many data collection tasks in the background so you can focus on the experiment at hand. Front-to-back workflow: Each step of the research process, from sample preparation to data management and analysis, has been considered to maximize rigor and reproducibility. Safety: Safety of the TEM is top priority – none of our systems require disassembly that would put the TEM at risk to enable different imaging techniques or perform maintenance. Temperature up to 1000°C Up to 1 bar H₂,O₂, CO, CO₂, CH₄, custom mixtures, humidity, organic vapors, and more Quiet, integrated residual gas analyzer Live physical drift mitigation, dose analysis, and offline data analysis All components are fully approved by the major microscope manufacturers to meet their rigorous standards for safety, compatibility, and reliability.
AXON Studio Data Processing Software The Studio module provides an intuitive and efficient means for processing and managing large TEM datasets all in one place. AXON STUDIO: STREAMLINED DATA PROCESSING AXON Studio provides researchers with advanced tools to efficiently analyze, organize, and share both in situ and conventional TEM data. Its intuitive interface simplifies the review of thousands of TEM images, enabling users to quickly identify the most critical data. By eliminating the primary challenges of post-processing, AXON Studio streamlines workflows and enhances productivity. Additionally, it fosters seamless collaboration among both local and remote colleagues, promoting meaningful and productive discussions. COMING SOON Cross Correlation with EDS Analysis For the first time, follow both the structural and compositional changes in a material throughout an in situ experiment. EXPLORE FASTER Plot and filter data in innovative ways to quickly locate key information and effortlessly identify trends. SHARE EASIER AXON Studio is a free download, enabling colleagues and stakeholders to conduct their own in-depth analyses and engage in more productive discussions PUBLISH SOONER Image stack and video export capabilities allow for the creation of data-rich visuals and videos, all in one platform, ready for publication FILTER FOR FAST SEARCHING All experimental metadata is indexed, meaning you can use any parameter to filter your data and find the most important information quickly. Filter by temperature, voltage, gas composition, magnification, or take advantage of AXON’s unique metadata to find the most dynamic or the best focused images in an instant. GET ORGANIZED USING COLLECTIONS Create subsets of data that are meaningful by using “collections”. This is a quick way to organize large amounts of data once you have identified the most important parts. Very similar to creating photo albums within your phone, collections help group data without duplicating and prepare datasets for sharing or publishing to a video. SHARE LARGE DATASETS EASILY The Pack-and-Share feature enables you to quickly reduce the size of a subset of data and zip up the file to share with your colleagues and collaborators. They will receive the images and all indexed metadata, including any attached files, notes, and tags, for a productive discussion. Since AXON Studio is free for all, all colleagues can visualize the data in the same manner. DATA LABELING AND ORGANIZATION While processing data, use tags to label parts of your session for better organization and easier retrieval later. These tags can be filtered and serve as helpful notes on the timeline, making it easier to share and explain your experiment to colleagues or (external) collaborators. INTEGRATED ANALYSIS TOOLS Studio offers various analysis tools, including measurement tools, live FFT view, customizable plotting, and a notebook with file management. These tools are accessible to anyone viewing a dataset in Studio, enabling thorough analysis and productive team discussions. PUBLISH VIDEOS AND IMAGE STACKS The publish tool enables you to create images stacks and videos all within the same software. Apply rolling and blocked averages, choose from several different files types (all FAIR/RDM approved), apply overlays, and more. Create the ideal set of images or video for your publication, presentation or report with just a few clicks of your mouse. AXON STUDIO COMES STANDARD WITH OUR AX IN SITU SOLUTION BUNDLES AXON MACHINE-VISION PLATFORM LIBRARY Published research using the AXON software solution. Release Notes Want to know what has changed in the newest version of AXON?
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TEM Management Software The Synchronicity module provides TEM data automation without taking away control so you can focus on what’s most important: your sample. AXON SYNCHRONICITY: YOUR VIRTUAL LAB ASSISTANT In situ TEM experiments involve managing numerous variables, and AXON Synchronicity provides automation without taking away your control. Whether you are a seasoned electron microscopist or an occasional user, Synchronicity transforms TEM data collection into a seamless process, empowering users of any skill level to efficiently acquire high-quality data. EXPLORE FASTER Automations help lower the learning curve for traditional and in situ TEM experiments PUBLISH SOONER More detail is captured per experiment, reducing the number of experimental iterations needed RDM/FAIR COMPLIANT A complete and transparent experiment history is recorded and maintained LIVE PHYSICAL DRIFT CORRECTION Synchronicity’s machine vision software monitors movement and automatically corrects for it live by moving the stage, adjusting the beam, adjusting the piezo (if available), and/or doing a digital correction. With correction in the x, y, and z planes happening in the background, you can take your hands off the controls and focus on your sample. Not to mention, you will leave the lab with a fully drift corrected dataset! LIVE PARAMETER PLOTS Monitor in situ parameters and unique AXON parameters live for a complete dashboard view of different experimental facets alongside images. LIVE FFT VIEW Monitor changes in material structure simultaneous to imaging live for efficient imaging optimization as well as a better understanding of material behavior under different conditions. ALL EXPERIMENTAL PARAMETERS INDEXED Over 200 experimental parameters from the TEM, the imaging system, and the Protochips in situ system are not only recorded and aligned with each image but also indexed to allow for easy filtering of images by parameter in our offline processing software AXON Studio. No need to worry about manually taking notes or aligning time stamps anymore! Enables compliance with FAIR Principles. INSTANT SESSION REVIEW Review all captured session data, completely drift corrected and aligned with all parameters, before removing the holder from the TEM. This will allow you to make critical decisions on the next steps instantly without having to spend days or weeks processing data first. DATA LABELING AND ORGANIZATION Tag parts of your TEM session with labels to organize your experiment better and find sections of it more easily later. You can even filter by these labels in our processing software! Cross Correlation with EDS Analysis For the first time, follow both the structural and compositional changes in a material throughout an in situ experiment. AXON Synchronicity comes standard with our AX in situ Solution bundles
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The Dose module ensures unparalleled reproducibility by automating the quantitative measurement and tracking of electron dose. AXON DOSE: PRECISION DOSE MANAGEMENT FOR RELIABLE RESULTS The electron beam can profoundly influence sample behavior, sometimes producing desired effects and sometimes introducing unwanted changes. Accurately understanding dose exposure across the sample at every stage of the experiment is essential for ensuring reliable results and reproducibility. AXON Dose is the first and only software of its kind that fully automates the calculation and recording of electron dose throughout the entire TEM workflow. By automating a traditionally labor-intensive and error-prone process, it establishes a new standard of reproducibility from one experiment to the next, and across multiple labs. INCREASE REPRODUCIBILITY A unique external Faraday cup and power supply deliver reproducible, quantifiable measurements, ensuring consistent results across any TEM SHARE EASIER All dose measurements are automatically aligned and indexed into the metadata, ensuring they’re readily accessible to anyone reviewing the data in AXON Studio INTERPRET FASTER Intuitive visualization tools aid in understanding dose effects on sample behavior EXAMPLES USING THE AXON DOSE MODULE VISUALIZE DOSE Colorful dose overlays introduce an entirely new way to visualize dose exposure. Electron dose is measured on a pixel-by-pixel basis and tracked throughout the entire experiment for both TEM and STEM modes, ensuring a detailed, continuous record. Colorized maps highlight actual exposure levels and indicate when approaching critical thresholds. IDENTIFY DOSE LIMITS Tools like spot fading quickly pinpoint when and where the electron beam first contacts your sample, helping you understand the beam’s influence based on dose rate or cumulative dose. Once dose thresholds are identified, the software allows you to set warning indicators. Colorful dose overlays then provide clear alerts for approaching dose levels. A BIRDS-EYE VIEW The dose navigation pane shows a low-magnification view of the stage and the same colorized dose maps show where the beam has been previously. Move to pristine areas with a simple double-click in the navigation pane to quickly begin a new experiment AXON Dose is an Additional Module for Our AX in situ Solution bundles Release Notes Want to know what has changed in the newest version of AXON?
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Controlled Illumination for Operando Environmental TEM Sol is a brand-new member of the Atmosphere AX family, enabling light-driven reactions to be studied under pressurized, gaseous environments at the nanoscale. The highly-published Atmosphere AX now enables nanoscale studies for a new set of research areas. Builds on the capabilities of Atmosphere AX: Supports the full capabilities of Atmosphere AX, like temperature control, gas pressure, compatibility, and mixing, enabling truly representative reaction environments. Well-characterized photoillumination workflow: Holder and ex situ calibration workflow optimized to quantify light power at the sample and maintain temperature accuracy, even during illumination. Flexible light integration: User-exchangeable light source input allows use of various wavelengths, making Sol adaptable to diverse experimental needs. The holder is compatible with most Thermo Fisher and JEOL TEM platforms. Seamless software integration: Photoillumination metadata is synchronized with experimental timelines using Protochips AXON machine vision software, reducing manual tasks and allowing you to focus on your research. Light source flexibility 350 nm - 2000 nm wavelength 0.0025 mm² Illuminated area ≥ 5000 mW/cm² Power density Integration of photoillumination metadata with AXON Closed-loop temperature control APPLICATIONS Photocatalysis Photochemistry Photovoltaics Optoelectronics Plasmonics SOL FOR ATMOSPHERE AX WORKFLOW Prep Preparation - Align optics - Ex situ power density calculation - Sample screening - Global scientific support Collect Collection - Live physical drift control - Dose quantification - Metadata indexing - Integrated notebook Analyze Analyze - Identify heat vs light induced reactions - Environmental control - TEM/STEM/EDX Optimized - Quiet gas analysis Publish Publish - Consolidated visualization - Fast filtering - Historical experimental records - RDM/FAIR compliant SOL FOR ATMOSPHERE AX COMPONENTS All components are fully approved by the major microscope manufacturers to meet their rigorous standards for safety, compatibility, and reliability. The Atmosphere AX solution uses custom-made microelectromechanical systems (MEMS) as sample supports to add temperature within the environmental holder. All MEMS devices (E-chips™) are designed, fabricated, and quality checked in-house, which gives Protochips an immense amount of control and oversight to ensure quality and proper functionality as well as keep cost minimized. Uniquely utilizing silicon carbide as a heating membrane instead of the traditional metal coil, Atmosphere E-chips provide superior uniformity and accuracy to other MEMS chips on the market.
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