Our proprietary pre-analytical platform ensures standardized, automated fresh sample preparation at the point of blood collection. SBS slides with a fixed monolayer of white blood cells for CTC identification and plasma for analysis of cell-free content are made available, with a walk-away protocol, by See.d.® No special tubes for blood collection, no specialized technicians, perfect sample integrity.
SmartBioSurface® slides enable stable immobilization of non-adherent cells while preserving the optical quality required for advanced fluorescence microscopy. Suspension-derived cells, including hematological cell lines and primary white blood cells, are often difficult to retain on conventional glass surfaces during fixation, staining, washing, and imaging procedures. This can compromise reproducibility, reduce image quality, and limit the effectiveness of high-resolution analyses. The proprietary titanium nanostructured coating promotes efficient cell retention directly on imaging-grade glass, allowing cells to remain firmly positioned throughout sample preparation and acquisition. At the same time, the coating maintains low background fluorescence and introduces no detectable imaging artifacts, ensuring high signal-to-noise ratios and accurate visualization of cellular and subcellular structures. SmartBioSurface® is fully compatible with standard immunofluorescence protocols and advanced fluorescence imaging technologies, including Confocal Laser Scanning Microscopy (CLSM), Structured Illumination Microscopy (SIM), Total Internal Reflection Fluorescence (TIRF), and Stochastic Optical Reconstruction Microscopy (STORM). In TIRF microscopy, the enhanced positioning of cells at the glass interface improves the visualization of membrane-associated and near-membrane structures while minimizing background signal. In STORM applications, the coating supports precise fluorophore localization and high-quality image reconstruction, enabling detailed analysis of nanoscale cellular features. Experimental studies have demonstrated robust cell retention throughout sample preparation and imaging workflows. The resulting combination of sample stability, optical performance, and imaging reproducibility makes SmartBioSurface® particularly effective for high-resolution and super-resolution microscopy of suspension cells. Among the tested configurations, the 20 nm titanium coating delivered the best performance in super-resolution imaging, providing improved localization precision and reconstruction quality. By combining reliable cell immobilization with excellent optical properties, SmartBioSurface® offers a versatile platform for advanced imaging applications in immunology, hematology, oncology, cell biology, and translational research. Fully compatible with multicolor immunofluorescence workflows, SmartBioSurface slides support a wide range of advanced microscopy platforms, including Confocal Laser Scanning Microscopy (CLSM), and super-resolution Structured Illumination Microscopy (SIM). The coating generates no detectable fluorescence artifacts and provides the optical stability required for high-quality image acquisition and reconstruction. By combining robust cell immobilization, excellent optical performance, and compatibility with advanced fluorescence imaging techniques, SmartBioSurface® slides offer a versatile platform for studying suspension-derived cells across applications in immunology, cell biology, hematology, and translational research.
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High-Plex Spatial Profiling of Suspension Cells SmartBioSurface® enables reliable high-plex spatial profiling of suspension cells, supporting integrated analysis of blood-derived and tissue samples within a unified workflow. When combined with advanced cyclic immunofluorescence platforms such as CellScape™, CellScape XR™, and Quanterix Spatial PhenoCycler®, SmartBioSurface® provides the stable cell immobilization required for repeated staining, washing, and imaging cycles while preserving image quality and assay reproducibility. Exceptional cell retention across multiple imaging cycles ensures consistent data generation and minimizes sample loss. In cyclic immunofluorescence workflows, SmartBioSurface® achieved approximately 98.5% cell retention after ten imaging cycles, supporting robust and reproducible biomarker analysis. Combined with the multiplexing capabilities of CellScape XR™, this enables high-content profiling with excellent signal quality. SmartBioSurface® has also demonstrated compatibility with the Quanterix Spatial PhenoCycler® platform, maintaining cell integrity throughout repeated staining and reporter-exchange procedures. Multiplex analysis of peripheral blood leukocytes generated specific and reproducible staining patterns across multiple immune markers, confirming the suitability of the workflow for high-dimensional immune profiling. By enabling the use of validated reagent panels and imaging chemistries across different platforms and sample types, SmartBioSurface® facilitates direct comparison between tissue sections and suspension-derived cells. This integrated approach provides a more comprehensive view of complex biological systems and supports advanced applications in immuno-oncology, immunology, infectious diseases, and translational research. Scalable, reproducible, and platform-compatible, SmartBioSurface® provides a robust foundation for next-generation spatial biology studies of non-adherent cells.
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Cellular fraction is gently stabilized on proprietary, nanocoated SBS slides for rare cell detection, including CTCs, while plasma is made available for the analysis of cell-free content. See.d® and SBS slides are for Research Use Only, not for use in diagnostic procedures.
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