By Applied NanoFluorescence LLC
Plate Reader Module For high throughput, high sensitivity experiments in near-infrared spectrofluorimetry A complete NS Super system with Plate Reader Module The newest add-on for our NanoSpectralyzer line of instruments, the Plate Reader module is designed for rapid measurement of SWCNT fluorescence from microwell plates. It is preprogrammed to work with 12, 48, 96, and 384-well plates using an intuitive graphical interface to define and control experiments. The NS Plate Reader Module offers unparalleled flexibility and speed for a wide range of applications. By sharing the excitation and detection hardware of an attached NanoSpectralyzer system, the Plate Reader accessory also provides exceptional value. Hardware features - Emission capture from 870 to 1600 nm - Continuously scannable excitation from 515 to 830 nm (with NS Super) - Multiple discrete excitation wavelengths (with NS1, NS2, or NS3) - Precisely selectable excitation times down to 10 ms - Compatibility with standard multi-well plates Software features - Powerful unified software for instrument control and data analysis - User-friendly, configurable selection of experimental parameters - Integrated software for automated (n,m) mapping of SWCNT samples
The NM1 is a specialized optical microscope system custom-designed for imaging individual single-walled carbon nanotubes through their intrinsic near-IR fluorescence. - A high quality modular inverted microscope with objective lenses - A focal plane array detector containing 320 x 256 InGaAs pixels, with sensitivity from 850 to 1700 nm, cryogenic cooling for dark current suppression, and calibrations to correct for sensitivity variations and nonlinearity - A fluorescence excitation system including three diode and/or DPSS lasers with electronic beam control, optics for beam combining, polarization control, and focusing - Appropriate optical filters for high contrast imaging of SWCNTs - A computer system for instrument control and data acquisition Versatile Visualization of Individual SWCNT with NIR Fluorescence Microscopy, Tsyboulski et al, Nano Letters (2005) 5, 975-979
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The spectral range of the standard NanoSpectralyzer can be expanded by adding a second thermoelectrically cooled multichannel spectrometer to detect NIR emission between approximately 1200 and 2100 nm with a resolution of approximately 8 nm. This instrument modification will also allow absorption spectra to be measured from 400 to 2100 nm.
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Additional NanoSpectralyzer Options - Vertical sample translation: For rapid, automated fluorescence mapping of DGU centrifuge tubes - Reduced sample volume capability: Allows measurements on 50 µL samples - Flow cells: Allow spectral monitoring of eluent streams - Plate reader: For rapid acquisition of data from multi-well plates - Extra NanoSpectralyze software license: For spectral data analysis on a stand-alone computer
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Introducing: The NS MiniTracer The NS MiniTracer is the latest addition to the innovative line of NanoSpectralyzer instruments from Applied NanoFluorescence, LLC. Its design is optimized for fast, easy, and highly sensitive analytical measurements on a variety of samples containing near-infrared fluorophores such as single-walled carbon nanotubes or quantum dots. The MiniTracer is ideal for quantitating SWCNTs in environmental and biological specimens and is also perfect for routine sample characterization in any SWCNT research lab. Features - Most affordable fluorometer for single-walled carbon nanotubes - Quick measurements with user-friendly software - Trace detection down to sub-nanogram levels; lowest LOD and LOQ - Wide dynamic range - Robust and compact design: approximately 165 x 215 mm (~6.5 x 8.5”) - Near-IR emission spectra from 900 to 1600 nm - Optional near-IR absorption spectra from 900 to 1600 nm - Rapid sequence acquisition for kinetic studies or eluent monitoring - Choice of one excitation wavelength The NS-MiniTracer is the newest and most affordable member of the NanoSpectralyzer family. Its robust, compact design includes one excitation laser and optics for capturing the characteristic near-IR fluorescence from fluorophores such as dispersed SWCNTs or PbS quantum dots. Optimized for the lowest possible optical backgrounds, the MiniTracer offers long integration times to provide exceptional detection sensitivity. It is the best instrument available for the selective detection and quantitation of trace amounts of SWCNTs in environmental and biological specimens. Operation of the NS-MiniTracer is also exceptionally easy and user-friendly. Custom software prompts the user to load blank and sample specimens, and automatically selects optimal data acquisition parameters. After acquisition is complete, a full emission spectrum is displayed along with the net spectrally integrated nanotube emission signal. This convenient numerical result is proportional to SWCNT content within a set of samples, and can be used to construct project-specific calibration curves. The NS-MiniTracer is also an ideal tool for any laboratory that needs to routinely characterize their SWCNT dispersions. It quickly captures emission spectra that reflect the composition, concentration, and individualization of a sample’s SWCNTs. And it gives even more spectral information with the affordable near-IR absorption option. Applied NanoFluorescence delivers the NS-MiniTracer as a complete turn-key system including a notebook computer pre-loaded with custom software for instrument control and data analysis. The system carries a full one-year warranty plus free software updates for three years. Please note the images of the instrument and software are of the prototype and the actual appearance may vary. NS MiniTracer Specifications Fluorescence excitation laser: 638 nm, 100 mW standard Other wavelengths available Fluorescence configuration: High numerical aperture epifluorescence Fluorescence spectral range: 900-1600 nm Near-infrared detector type: 512 element TE-cooled InGaAs array Limit of detection: < 10-9 g single-walled carbon nanotubes Minimum sample volume: 120 μL Absorption light source: Stabilized tungsten-halogen lamp Absorption spectral range: 900-1600 nm Absorption spectral resolution: 6 nm Absorption ceiling: 3 AU Absorbance noise (rms): < 2 x 10-4 AU at 0 AU for 10 s integration Power consumption: 40 W max (excluding computer) Main Module dimensions: 6.5" W x 8.5" D x 5.5" H (165 x 215 x 140 mm) Spectrometer Module dimensions: 7.2" W x 4.3" D x 1.9" H (182 x 110 x 47mm) System weight: 8 lbs (3.7 kg), excluding notebook computer Absorption capability is optional Near-infrared fluorescence spectrum (900-1600 nm) with 638 nm excitation Sequence acquisition of near-infrared fluorescence spectra (900-1600 nm) with 638 nm excitation Optional: near-infrared absorption (900-1600 nm) Sample is a single-walled carbon nanotube suspension in D2O MiniTracer Flyer MiniTracer Specification Sheet Product Catalog
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