Introducing Lumi804 – Next Generation Radiometal Chelation Lumiphore has introduced its new 1,2-HOPO macrocyclic BFC for TRT use dubbed Lumi804 TM. This single molecule chelate can handle a variety of radio-metals and is available for licensing and collaboration opportunities. One mBFC – Multiple Radiometals Retaining a small but important degree of flexibility is a feature of all of our macrocyclic BFCs. Shown above are the crystal structures of Eu-, Lu- and Zr-Lumi804 parent mBFCs, demonstrating how a wide range of metal ion sizes can all be accommodated by a single partially flexible chelator. Additionally, Europium is highly sensitized by Lumi804 with a quantum yield approaching 30% - highest available for aqueous environment. This an other core features are enabling in vitro assay and analysis utilizing Time Resolved Fluorescence (TRF) or TR-FRET. Stable In-Vivo Chelation Complex stability was probed with 1:1 metal:Lumi804 complexes, HPLC purified, incubated in 250 mM DTPA at pH = 7.4 for up to 18 days. As Lumi804 has a characteristic absorption spectra differentiating the Prescence or absence of chelated metal, this was monitored for up to 18 days at 24C. Lumi804-Eu binds and sensitizes the luminescent metal europium, which emits at different wavelength than terbium, permitting simultaneous multi-channel fluorescent testing. Both Lumi4®-Tb and Lumi804™-Eu are used in high value homogenous assays.
