By Novascan Technologies, Inc.
Novascan Technologies offers a variety of unmodified and modified AFM, but also welcomes the opportunity to discuss custom probe and surface modifications. Custom modifications may include unique particle attachment and alternative chemical and protein conjugations. Novascan's Probe Laboratory modifies commercially available AFM probes. Standard probe composition and spring constant information appears below. In most cases, the Probe Lab can also modify cantilevers supplied by clients. Please inquire as to the feasibility of using your probes for a given probe modification. Probe Type/Spring Constant - Silicon (N/m): 0.03, 0.05, 0.08, 0.65, 0.95, 1.75, 4.5, 7.5, 14 - Silicon Nitride (N/m): 0.06, 0.12, 0.32, 0.58 - Other probes can be special ordered. Particle Materials/Sizes Metallic Coatings - Borosilicate Glass (µm): 2, 5, 10, 12, 15, 20 Gold - SiO2 (µm): 0.6, 1, 2.5, 5, 10, 20 Platinum - Polystyrene (µm): 1, 4.5, 10, 25, 45 - Polyethylene (µm): Please Inquire - Tungsten (µm): 5, 10. Defined Chemistries - Alkanethiols: COOH, CH3, NH2, OH, Succinimide - PEG Linkers: PEG/COOH, PEG/NH2, PEG/Maleimide, PEG/Biotin - Silanes: APTES - Biotin/Streptavidin/Neutravidin. Protein and Antibody Conjugation - Novascan's probe technicians have developed several protocols for the conjugation of proteins to unmodified and particle modified AFM probes. The addition of linking molecules such as polyethylene glycol (PEG) is often required. Please inquire as to the feasibility of using specific proteins.
