While LifeSensors offers both yeast (SUMOpro) and human (SUMOpro3) tags with corresponding SUMO proteases 1 and 2, respectively, in mammalian expression endogenous SUMO proteases can cleave the protein, preventing expression and purification. SUMOstar is designed to prevent the above issue by using an engineered SUMO tag to prevent cleavage and preserve the enhanced protein production properties of SUMO. Isolated SUMOstar fusions from eukaryotic cells can be cleaved efficiently via SUMOstar protease in vitro. Service Highlights: SUMOstar prevents cleavage in eukaryotic cells preserving key properties for enhanced expression; Dramatically enhances protein expression and solubility, reducing costs in costly mammalian cells; SUMOstar protease enables removal of tag to generate native N-terminini
Compiled from http://www.lifesensors.com
