SARomics Biostructures offers NMR-based comparative analysis of higher-order structures. The details can be found on our antibody structure and epitope mapping services and protein NMR spectroscopy services pages. We use the 2D 13 C NMR method described above. The fingerprint spectra of the methyl groups of the originator antibody and its biosimilar are registered and a analysed, revealing any differences directly between the two.A significant advantage is that no extra labeling is required for these experiments, meaning no additional material needs to be produced. Molecules as large as whole antibodies can be analyzed using this approach. Additionally, NMR higher-order structure analysis can be used for formulation optimization and batch comparison. In such cases, the biomolecules can be studied in the actual formulation buffer. Depending on the biomolecule, around 1-2 mg of protein can be sufficient for HOS analysis.
