These CNAST or Mellon College of Science resources are available for general use by CNAST members. ABI 2720 PCR Thermal Cycler Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider ABI 310 Genetic Analyzer Capillary Electrophoresis System Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider ABI 3130xl Genetic Analyzer Capillary Electrophoresis System Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider ABI ViiA7 qPCR Machine Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Access to Modified DNA/RNA Sequences - For DNA up to 35 mers and RNA up to 30 mers - For incorporating modifications for which commercial specialty phosphoramidites are available (via GlenResearch) Das Lab, MI 742 Contact: Subha Das Agilent TapeStation - For rapid gel electrophoresis and automated analysis of RNA and DNA abundance, size, and integrity McManus Lab, MI 255 Contact: Joel McManus AKTA Explorerer FPLC Liquid-phase Chromatography System Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Analytical and Semiprep HPLC Achim Lab, MI 835/839 Contact: Catalina Achim ASI MS-2000 Programmable Translation Stage Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Beaglehole Variable-Angle Ellopsometry System Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Beckman IC100 Murphy Lab Automation Suite, MBIC 1st floor Contact: Robert Murphy Beckman-Coulter P-ACE MDQ Automated Capillary Electrophoresis Systems - With UV, two-color LIF detection and photodiode array detection Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Biacore Surface Plasmon Resonance Instrument Das Lab, MI 742 Contact: Subha Das Bio-rad Gel Dryers - Model 583 Das Lab, MI 742 Contact: Subha Das Bio-Rad Protean Llxi Slab Gel Electrophoresis System Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Bruker Nanoscope V MultiMode AFM System Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Cary 300Bio UV-Vis Spectrophotometer - With temperature control Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Cary Eclipse Fluorescence Spectometer - With stopped-flow accessory, peltier multi-cell holder, and temperature control Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Cell Analyzer - 88/640 lasers with 96-well plate sipper attachment Intellicyte/Accuri C6 Cytometry Center, MBIC 1st floor Contact: Yehuda Creeger Cephied SmartCyclyer PCR workstation - With 16 sample chambers and 4 channel optical detection Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Circular Dichroism Center for Molecular Analysis, MI 555 Contact: Mark Bier Coherent Optics QE Pro High-Performance Spectrometer Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Differential Scanning Calorimetry Evilevitch Lab, WH 6409 Contact: Alex Evilevitch Eppendorf 5415C Ultracentrifuge Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Eppendorf 5810R Ultracentrifuge Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Fluorescence Correlation Spectrometer - Zeiss Confocor 3 Unit Microscopy Facility, MBIC 2nd Floor Contact: Haibing Teng Fluorescence Spectrometers - Cary Bio, Equipped for melting curve analysis (2) - Photon Technology Inc Armitage Lab, MI 726 Contact: Bruce Armitage Fluorescent Activated Cell Sorter - FACSVantage, 4 lasers (405, 488, 532, 640) Cytometry Center, MBIC 1st floor Contact: Yehuda Creeger Forte Bio Red Evanescent Surface Sensor Washburn Lab, MI 814 Contact: Newell Washburn Gel Electrophoresis Equipment - For agarose and polyacrylamide gels - including large plates/gels – for gels 20cmx35cm - Reserved for use with nucleic acids (no proteins) Das Lab, MI 742 Contact: Subha Das Gel Filtration Chromatography Bruchez Lab Coldroom, MI 755 Contact: Marcel Bruchez Gel/Culture Plate Scanner - Typhoon 9700 Location TBD (2nd floor currently) Contact: Marcel Bruchez Isothermal Titration Calorimetry Rule Lab, MI 246 Contact: Gordon Rule JASCO J-810 Circular Dichroism Spectrometer Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Jobin-Yvon Fluorolog-3 Florescence Spectrometer - With TIRF accessory Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider KSV 5000 Langmuir-Blodgett Trough - With dipping accessory, Wilhelmy plate, and surface potential accessory Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Labsmith Microflluidics Toolkit - Programmable DC power supply and chip prototypes Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Malvern Zatasizer Nano Dynamic Light Scattering System Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Mass Spectrometer - Q-TOF-ESI/APCI/Nanospray - LCQ-ESI/APCI Ion Trap - MALDI Center for Molecular Analysis, MI 555 Contact: Mark Bier Mermade 4 DNA/RNA Synthesizer (Bioautomation) Das Lab, MI 742 Contact: Subha Das Metamorph Imaging Software Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Nanodrop - Scanning UV-vis Spectrophotometer - For spectra with small sample (2-3 μL) volumes Das Lab, MI 742 & Shared Chemical Engineering Lab, DH 2203 Contact: Subha Das & Jim Schneider Nicolet Magna-IR 560 IR Spectrophotometer - With MCT and DTGS detection Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Nikon TE2000U Inverted Microscope - With high-resolution CCD camera (Photometrics Quatrum EM) and integrated laser system with interlocks Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider NMR Spectrometers Pittsburgh NMR Center for Biomedical Research, MI 1st Floor Apply To Use Facilities Ocean Optics QE Pro High-Performance Spectrometer Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Perkin Elmer Spectrum CT Imaging System & Surgical Suite - IVIS/CT Small animal fluorescent/luminescent/CT imaging instrument MI Vivarium Point-Scanning Spectral Confocal Microscopes - Zeiss 510 Meta 2x Shared Imaging Facility, MBIC 2nd floor Contact: Haibing Teng Potentiostat for Electrochemistry Measurements Achim Lab, MI 835/839 Contact: Catalina Achim Preparative Ultracentrifuge MBIC Common Equipment Rm, 2nd floor Contact: Haibing Teng Spectral Fluorescence Plate Reader - Tecan Safire 2 MBIC, 2nd floor Contact: Haibing Teng Spectral Fluorescence/Luminescence Plate Reader with Anisotropy - Tecan M1000 Bruchez Lab, MI 755 Contact: Marcel Bruchez SpectraMax M2 UV-Vis Fluorescence Microtiter Plate Reader System Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Spinning Disk Confocal Microscope with Live-Cell Apparatus Shared Imaging Facility, MBIC 2nd floor Contact: Haibing Teng Stopped flow UV Vis Achim Lab, MI 835/839 Contact: Catalina Achim Synergy H1 Hybrid Plate Reader System Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Teledyne Isco Comniflash Rf 200i Flash Chromatography System Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Tokai Hit Transparent Thermoplate Stage Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider UV Vis Spectrometers - Cary 200 - Cary 50 - Cary 300, Equipped for melting curve analysis (2) Armitage Lab, MI 726 Contact: Bruce Armitage UVP Bio-DocIt Transilluminator - With CCD Image Capture Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Waters Alliance 2690 Analytic HPLC System - With heated column, PDA, fluorescence, and DLS detection Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider Waters Delta 600 Semi-prep HPLC System - With PDA detection and fraction collector Shared Chemical Engineering Lab, DH 2203 Contact: Jim Schneider
