By Breault Research Organization
The main focus of this course is volume light scattering and fluorescence with emphasis on, but not restricted to applications in life sciences. The tutorial gives participants the knowledge and skill needed to model the optical performance of biomedical systems and instruments with ASAP. Participants balance theory with hands-on experience by applying each lesson in a series of practice sessions. COURSE OVERVIEWThe course covers the simulation and analysis of volume light scattering and fluorescence, based on ASAP. A thorough introduction into the underlying physics is provided. Basic knowledge about light scattering and fluorescence is not required. Basic concepts are explained but theoretically and in practice sessions. Standard measurement techniques are discussed and simulated using ASAP. PRE-REQUISITES - Completion of the ASAP Introductory Tutorial or equivalent knowledge and experience - Ability to build and view elementary systems with surface and edge-based entities - Ability to define and trace rays from various sources, and perform power, irradiance, and intensity calculations - Ability to comfortably write and run command scripts and macros COURSE OUTLINE Light scattering by a single particle - Introduction into the phenomenology of light scattering - Observable quantities: cross-sections, efficiencies and the phase function - Mie theory Scattering Media - Quantities to measure the amount of scatters: number density, volume concentration and area obscuration per length - Particle size distributions Multiple Scattering - Radiative transfer and Monte Carlo simulation - Phenomenological radiative transfer equation and the Henyey-Greenstein model - Inhomogeneous media - User-defined scattering models - Analysis of light propagation and absorption inside a scattering media using VOXELS Fluorescence - Physical background - Fluorescence measurement methods - Simulation and analysis methods This a three-day tutorial and participation is limited. If you are unsure you meet the course requirements, please contact the instructor before enrolling.
