cellZscope systems are the ideal tools for studying epithelial or endothelial cells in vitro. cellZscope systems allow automated, long-term monitoring experiments with up to 96 different cell cultures simultaneously. The transepithelial/transendothelial electrical resistance (TEER) and optionally the capacitance (C cl) of various types of barrier-forming cells cultured on permeable membranes can be analyzed in real-time. The cellZscope is easy to operate and has a broad range of possible applications. Impedance Based Cell Monitoring – From Short-Term Tight Junction (TJ) Dynamics To Long-Term Layer Formation. Applications include: Influence of different concentrations of EGTA on the tight junction network (TEER) of Madin-Darby canine kidney (MDCK) cells; Control of layer formation and differentiation of Caco-2 cells over several weeks; Monitoring of the junction formation of Madin-Darby canine kidney (MDCK) cells; Dose dependent response of barrier (TEER) and cell layer capacitance C cl on MBCD; Barrier of cultured primary endothelial cells derived from porcine brain microvessels dependent on the presence of hydrocortisone; Influence of serum on the cell layer morphology of cultured primary epithelial cells derived from porcine choroid plexus; Automated and continious measurement of the TEER with the cellZscope compared to taking single measuring points with a hand-held device; Advantages of a non-interfering measurement in the incubator compared to disruptive influences during manual handling; Cell membrane lysis (MDCK-I) due to different concentrations of Saponin; Monitoring of fast processes with the cellZscope3.
