AutoEV is a CODI application that connects the ONI Nanoimager to CODI cloud-based analysis platform, enabling Extracellular Vesicle (EV) characterization using the ONI Application Kit TM: EV Profiler 2. AutoEV consists of several advanced features that allow users to perform super-resolution imaging and analysis of an entire 4-lane EV Profiler 2 chip with reduced hands-on time and a robust pipeline. Nanoimager requirements: 1. Requires active internet connection on the Nanoimager PC 2. Requires a standard Nanoimager configuration: Lasers: 488, 561 and 640; Nanoimager Mark III (contact ONI if unsure) Prior to using AutoEV - Placing the chip holder on the Nanoimager: AutoEV comes with a chip holder that makes the Nanoimager feel like it was made for EV Profiler 2 chips. It also allows AutoEV to know where each lane is without any specific calibration. With the Nanoimager still switched off, place the chip holder on the Nanoimager before opening the CODI System App (CSA). 1. Place the chip holder on the Nanoimager stage as shown in the illustration below. 2. Place the EV Profiler 2 chip or bead slide (with magnets) inside the chip holder. Installation: To run the EV Profiler 2 kit, you will need to register to ONI's CODI software platform, download the CODI System App and use AutoEV app.
