From sample to answer EV Profiler 2* is our second generation reagent kit for visualization and phenotyping of extracellular vesicles using dSTORM microscopy on the Aplo Scope and Nanoimager. The new kit introduces our Pan-EV stain, internal cargo detection as well as major improvements to reproducibility and EV capture efficiency. Capture stunning super-resolution images of EVs labeled with Pan-EV and Tetraspanin Trio for high confidence EV identification and sizing (with accuracy comparable to TEM), while using the only tool on the market that can characterize the spatial distribution and colocalise up to three biomarkers at the individual EV level. When combined with our newest software offering, AutoEV, your system will be calibrated and optimized to acquire and analyze a 4-lane chip automatically and deliver a comprehensive report of EV size and positivity for each individual lane in 90 mins. What's in the kit? - 3 Assay Chips, each with four lanes - Surface Reagent - A selection of capture antibodies to choose from - Pan-EV Detection and Tetraspanin Trio - Fixative, Wash Buffer and Staining Buffer - Permeabilization Buffer - dSTORM Imaging Buffer Optional add ons - 3 Color Tetraspanin Antibody Panel (CD9, CD63 and CD81) - HEK EV Standard Control (EVs from HEK culture media) *EV Profiler 2 has a shelf life of two months. AutoEV requires a CODI account and an active internet connection. Access to AutoEV without an internet connection will come later. EV Profiler Kit 2 and AutoEV are compatible with and Aplo Scope or Nanoimagers manufactured from 2021 on. Confidently identify your EVs Be confident your EVs are really EVs EV Profiler 2 includes ONI's Pan-EV Detection and pooled Tetraspanin Trio reagents. This combination ensures the exclusion of contaminating particles or antibody aggregates from the analysis. Key outputs: - Identification of true EVs through the utilization of ONI's Pan-EV Detection reagent - Tetraspanin Trio Detection reagents ensures precise analysis, excluding contaminating particles and antibody aggregates - EV Profiler 2's advanced features support the 2023 MISEV guidelines enabling orthogonal characterization of EV samples with a robust and reliable EV analysis workflow Visualize & size like TEM Visualize and size your EVs accurately, quickly and easily Quick and unbiased visualization and sizing of EVs through the pan-EV stain, reveals both membrane and shape details for a comprehensive analysis of their dimensions and characteristics. Key outputs: - Visualization of EVs providing a clear and detailed view of the EV membranes with sizing comparable to TEM, but faster and from your labs benchtop - The pan-EV stain labels EV membranes, ensuring unbiased sizing for accurate dimension information - Unveil the shape of EVs, and have a more comprehensive understanding of their characteristics Profile surface & cargo markers Detect and visualize biomarkers inside or on the surface of EVs Detect and visualize biomarkers inside and on the surface of EVs, allowing for efficient phenotyping and insight into biomarker clustering. Key outputs: - Label samples in stunning 3-color super-resolution and assay for cargo or surface biomarkers all in one kit - Reveal the clustering of biomarkers on or within EVs, offering a more detailed understanding of their distribution Co-localize up to 3 channels Co-localize up to three biomarkers EV Profiler 2 detects biomarkers in up to three channels, enabling measurement of single, double, and triple positivity on individual EVs with a 10% SD between assay chips. Key outputs: - Assay for size and positivity by combining Pan-EV, Pooled Tetraspanin Trio, and a biomarker of your interest or analyze each independent Tetraspanin in its own channel - A validated antibody panel including anti-CD9 (488), anti-CD63 (561) and anti-CD81 (647) can be purchased as an add on - Biomarker positivity across multiple chips and lanes (left) falls below 10% standard deviation when measuring single, double, and triple positivity for CD9, CD63, and CD81
This kit contains two single-domain antibodies (sdABs) for DNA-PAINT as well as the necessary imager strands and buffers that are required for sample preparation and imaging. Each sdAB can be coupled to a single DNA-PAINT docking site and two sdABs can bind one primary antibody, resulting in two docking sites per primary antibody. The reagents are tested and optimized for the Nanoimager. Simply add your primary antibodies of interest and you are ready for 2 color DNA-PAINT imaging.
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This kit (MASSIVE-sdAB ONI 2-PLEX Kit) includes two single-domain antibodies (sdABs) for DNA-PAINT, along with imager strands and buffers for sample prep and imaging. Each sdAB binds a DNA-PAINT docking site, with two sdABs per primary antibody. Optimized for ONI microscopes—just add your primary antibodies for 2-color DNA-PAINT imaging.
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When combined with our newest software offering, AutoEV, your system will be calibrated and optimized to acquire and analyze a 4-lane chip automatically and deliver a comprehensive report of EV size and positivity for each individual lane in 90 mins.
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Simple. Engineered. Dynamic. Powerful. A new era in super-resolution imaging Meet Aplo Scope – ONI’s new single-molecule super-resolution microscope designed for effortless molecular imaging. Perfect for researchers of all levels, Aplo Scope combines advanced imaging, precise laser control, and intuitive software to deliver high-quality results, from research on the foundations of life to drug discovery, biomarker research, and disease studies. With its compact design, Aplo Scope transitions seamlessly from live-cell to super-resolution imaging down to 15 nm resolution, without complex setup.Its unparalleled ease of use and integration with the Aplo Platform make it ideal for uncovering molecular interactions and driving breakthroughs. Simple Designed to simplify complex microscopy with one easy-to-use tool. Start imaging quickly, generate data with ease, and unlock answers quicker than ever before. Engineered Robustly engineered for stability, consistency, and reliability, to maximize reproducibility across experiments and users. Dynamic Seamlessly transition between live-cell imaging and super-resolution without reconfiguration for unmatched flexibility in one platform. Powerful Compact footprint, yet powerful enough to enable complex scientific inquiry. Aplo Scope is designed to fit into your lab, not the other way around. The new and groundbreaking SMLM microscope More Channels, More Insights Flexibility Redefined Maximize Throughput Homogeneous Illumination More Channels, More Insights Multicolor imaging reveals biological context Achieve maximal spectral separation with minimized cross-talk and background thanks to an optimized filter set including single, dual, and quad options. Using only 4 lasers, you can generate detailed multi-color panels of 5 or more labels to elucidate complex protein and particle interactions that span both large pan-cellular structures and nanoscale molecular features. Leverage the rapid laser switching with multiband filters for nearly simultaneous multi-channel imaging. Flexibility Redefined Live cell to super-resolution imaging Effortlessly capture dynamic cellular events in real-time, then zoom into molecular details with super-resolution precision — all within a single, intuitive platform designed to enable your cutting-edge science. From low-power live-cell imaging to high-power super-resolution imaging, the Aplo Scope eliminates the need for hardware reconfiguration. Aplo Scope includes unique illumination stability, even at extremely low power (<0.01 kW/cm2), to minimize phototoxicity in extended live cell imaging sessions. At the other end, high laser powers enable rapid, super-resolution imaging to uncover molecular level details. Maximize Throughput Expansive field-of-view Aplo Scope contains a 110 by 110 micrometres field-of-view, in both diffraction-limited and single-molecule localization microscopy. The expansive field-of-view significantly increases the chances of capturing rare events and elusive phenotypes. Detect ultra-rare cell populations and particle biomarkers with unmatched throughput, accelerating groundbreaking discoveries and driving transformative breakthroughs. Homogeneous Illumination Variation that reflects biology Aplo Scope produces a consistently flat, symmetric illumination across the entire field of view, vital for statistical and comparative intensity analysis. With consistent resolution and precision edge to edge, every frame produces more data, maximizing throughput of critical and sensitive assays. This enables quantitative molecular studies to better understand crucial mechanisms in disease and drug discovery studies. Key features of Aplo Scope Next-gen lasers Power density continuously adjustable from 0.01 through 3.325 kW/cm2 Light engine Packed with technology to enable diffraction-limited and SMLM imaging Illumination Illumination angle control from Epi to HILO and TIRF. Consistent symmetric illumination across the field-of-view Temperature control Precise temperature control up to 40°C Filter Wheel Four laser channels can be interlaced with any combination of emission filters to perform multicolor imaging, easily generating 5-color overlays Camera Sensitive next-gen CMOS camera for low noise, high-speed imaging Focus Z-lock Rapid and precise focus with locking technology to minimize Z-drift Field-Of-View 110 by 110 micrometres in both diffraction-limited and single-molecule microscopy Stage Fully automated piezo stage fits most microscopy slides and plates Mechanical stability Integral dual-stage isolation system minimizes vibration Sample enclosure Integral lid interlock ensures user safety, class 1 laser instrument Aplo Scope Aplo Scope is the flagship innovation behind the Aplo Platform: solid performance in a versatile, easy-to-use microscope. Experience a range of imaging modalities with this highly compact microscope designed to simplify multicolor super-resolution microscopy. Aplo Scope allows you to start imaging right away, no dark room or optical table is required. Its integrated software enables fixed or live imaging anywhere, and seamless up to 5 color multimode images! How much training is needed to get started using Aplo Scope? With unparalleled ease-of-use and simplicity, Aplo Scope has been designed to eliminate barriers to entry into the traditionally complex world of super-resolution microscopy and single-molecule imaging, and allow users of any skill level to utilize the instrument within one day of training. What are the dimensions of Aplo Scope? The footprint of the microscope body is as small as a standard laptop 220 mm (w) x 421 mm (d) x 242 mm (h), the light engine measures 215 mm (w) x 420 mm (d) x 456 mm (h) and looks like a computer tower. They’re attached to each other by a 1.5 m long cable and, importantly, can be used on any bench or desk without needing a dark room or optical table. Is Aplo Scope compatible with third-party kits and data analysis tools? Yes, samples can be stained with any super-resolution microscopy-compatible dye and imaged using Aplo Scope. However, this would require manual acquisition and analysis within CODI, or a third party application. ONI offers end-to-end kits where sample preparation, image acquisition, analysis and reporting are all integrated into a workflow, making imaging a seamless and more automated experience.
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