Automation to accelerate discovery Aplo Flow The ecosystem for simplified fluidic automation Aplo Flow combines automated sample preparation with programmable fluidics to deliver reliable, end-to-end super-resolution workflows. Integrated with the Aplo Platform, it minimizes hands-on time, improves reproducibility, and streamlines applications from DNA-PAINT and exchange imaging to extracellular vesicle (EV) and lipid nanoparticle (LNP) characterization. Enhanced throughput and modular architecture Aplo Flow's modular architecture decouples sample preparation from imaging, enabling parallel workflows for greater throughput. Aplo Flow prepares up to 16 samples in 4.5 hours with up to 85% less hands-on time, powering scalable DNA-PAINT experiments and standardized sample-to-answer workflows for EV and LNP research. One system, two modes of operation PREP Fluidics Driver Automated sample preparation Automate parallel sample preparation directly on the Aplo Flow deck. Process up to 16 samples with up to 85% less hands-on time while improving reproducibility and reducing time to results. Combined with ONI Application Kits™, Aplo Flow delivers complete sample-to-answer workflows for quantitative single-particle characterization of LNPs and EVs. LNP Profiler™ + AutoLNP - Characterize up to 16 LNP samples/day - Quantify size, cargo loading, integrity, and ligand abundance EV Profiler™ 2 + AutoEV - Characterize up to 12 EV samples/day - Measure EV heterogeneity at the single-particle level Boundless fluidic microscopy Aplo Flow transforms complex super-resolution assays into fully automated, reproducible workflows. Programmable fluid handling and proprietary microfluidic consumables enable scalable DNA-PAINT, spatial biology, live-cell uptake, and dosing studies with minimal hands-on intervention. By integrating fluidics, imaging, and analysis into one platform, Aplo Flow delivers reliable unattended operation without compromising image quality or repeatability. Engineered thermal control Long-duration imaging with exceptional drift stability. Purpose-built consumables Seamless setup with reliable fluid exchange. Flexible workflow software Create, automate, and scale complex assays. Key features Maximized throughput Decoupled fluidics and imaging increases speed, processing up to 16 samples in one day Automated fluidics Hands-off operation that eliminates manual error and experimental variance Standardized workflows Seamless sample-to-answer nanoparticle characterization workflows Unified platform Fully automates complex microscopy, infeasible without integrated fluidics Discover Aplo Flow ONI Application Kits™ Reagent kits for visualization and characterization of EV or LNP samples with single-particle sensitivity Pipettor Provides precise, automated fluid handling, enhancing efficiency and reproducibility Chip holder Custom ONI fluidics chip holder (four positions) Dual tip rack storage Sufficient tips for uninterrupted preparation of up to 16 samples Waste capture station Collects used tips and sample waste for disposal Custom fluidics pass-through Fluidic tether pass through for sequential reagent perfusion Light safe windows Helps to block harmful light from damaging samples Custom reagent holder Workflow specific labware positions for intuitive deck loading Fluidics driver All-in-one hardware to automate almost any fluidic-based assay Transfer station Scalable, parallel sample processing, including on deck temperature control for sensitive reagents How does Aplo Flow improve workflow efficiency? Aplo Flow enhances workflow efficiency by fully automating sample preparation for any of ONIs custom reagent kits. With less than 30 minutes of hands-on time, even a novice scientist can prepare up to 16 samples with high reliability and consistency. Instead of spending hours preparing samples by hand, Aplo Flow users are free to image other samples, analyze yesterday's dataset, or plan their next experiment. More answers in less time; the true meaning of efficiency. How many samples can Alpo flow process per run? Process anywhere from 1 to 16 samples using ONI's particle characterization kits (3x chips with 4x lanes on each), indicating custom experimental conditions for each lane before starting the experiment. Each ONI Cell Flow Chip can accommodate up to 4 samples in a single automated fluidics assay. How adaptable is Aplo Flow to different research needs? Aplo Flow's modular design provides sample level flexibility control in both end-to-end particle characterization workflows and unparalleled versatility for bespoke fluidic assays. Combined, Aplo Flow can cater to virtually limitless experimental designs, while ensuring adaptability and robust scalability in a diverse set of scientific applications. What support and resources are available for users of Aplo Flow? Users of Aplo Flow have access to comprehensive support and resources, including training materials, technical assistance, and software updates. This ensures that researchers can maximize the capabilities of Aplo Flow and achieve their scientific goals efficiently and effectively. Can I use Aplo Flow to build DNA-PAINT workflows? Yes, the Aplo Platform is designed to accommodate complex assay types exactly like DNA PAINT and exchange PAINT. The Aplo SDK paired with robust Aplo Flow fluidics hardware is well-suited to execute assay types that are typically too tedious to conduct manually, due the need for repeated user intervention over many hours. With Aplo, you can design an experiment in basic python code, place the necessary reagents on the Aplo Flow deck, and trust the Aplo Platform to handle the rest. DNA PAINT experiments with 10+ exchange cycles across up to four (4) samples can be easily automated and run "lights-out".
This kit contains two single-domain antibodies (sdABs) for DNA-PAINT as well as the necessary imager strands and buffers that are required for sample preparation and imaging. Each sdAB can be coupled to a single DNA-PAINT docking site and two sdABs can bind one primary antibody, resulting in two docking sites per primary antibody. The reagents are tested and optimized for the Nanoimager. Simply add your primary antibodies of interest and you are ready for 2 color DNA-PAINT imaging.
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This kit (MASSIVE-sdAB ONI 2-PLEX Kit) includes two single-domain antibodies (sdABs) for DNA-PAINT, along with imager strands and buffers for sample prep and imaging. Each sdAB binds a DNA-PAINT docking site, with two sdABs per primary antibody. Optimized for ONI microscopes—just add your primary antibodies for 2-color DNA-PAINT imaging.
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When combined with our newest software offering, AutoEV, your system will be calibrated and optimized to acquire and analyze a 4-lane chip automatically and deliver a comprehensive report of EV size and positivity for each individual lane in 90 mins.
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Simple. Engineered. Dynamic. Powerful. A new era in super-resolution imaging Meet Aplo Scope – ONI’s new single-molecule super-resolution microscope designed for effortless molecular imaging. Perfect for researchers of all levels, Aplo Scope combines advanced imaging, precise laser control, and intuitive software to deliver high-quality results, from research on the foundations of life to drug discovery, biomarker research, and disease studies. With its compact design, Aplo Scope transitions seamlessly from live-cell to super-resolution imaging down to 15 nm resolution, without complex setup.Its unparalleled ease of use and integration with the Aplo Platform make it ideal for uncovering molecular interactions and driving breakthroughs. Simple Designed to simplify complex microscopy with one easy-to-use tool. Start imaging quickly, generate data with ease, and unlock answers quicker than ever before. Engineered Robustly engineered for stability, consistency, and reliability, to maximize reproducibility across experiments and users. Dynamic Seamlessly transition between live-cell imaging and super-resolution without reconfiguration for unmatched flexibility in one platform. Powerful Compact footprint, yet powerful enough to enable complex scientific inquiry. Aplo Scope is designed to fit into your lab, not the other way around. The new and groundbreaking SMLM microscope More Channels, More Insights Flexibility Redefined Maximize Throughput Homogeneous Illumination More Channels, More Insights Multicolor imaging reveals biological context Achieve maximal spectral separation with minimized cross-talk and background thanks to an optimized filter set including single, dual, and quad options. Using only 4 lasers, you can generate detailed multi-color panels of 5 or more labels to elucidate complex protein and particle interactions that span both large pan-cellular structures and nanoscale molecular features. Leverage the rapid laser switching with multiband filters for nearly simultaneous multi-channel imaging. Flexibility Redefined Live cell to super-resolution imaging Effortlessly capture dynamic cellular events in real-time, then zoom into molecular details with super-resolution precision — all within a single, intuitive platform designed to enable your cutting-edge science. From low-power live-cell imaging to high-power super-resolution imaging, the Aplo Scope eliminates the need for hardware reconfiguration. Aplo Scope includes unique illumination stability, even at extremely low power (<0.01 kW/cm2), to minimize phototoxicity in extended live cell imaging sessions. At the other end, high laser powers enable rapid, super-resolution imaging to uncover molecular level details. Maximize Throughput Expansive field-of-view Aplo Scope contains a 110 by 110 micrometres field-of-view, in both diffraction-limited and single-molecule localization microscopy. The expansive field-of-view significantly increases the chances of capturing rare events and elusive phenotypes. Detect ultra-rare cell populations and particle biomarkers with unmatched throughput, accelerating groundbreaking discoveries and driving transformative breakthroughs. Homogeneous Illumination Variation that reflects biology Aplo Scope produces a consistently flat, symmetric illumination across the entire field of view, vital for statistical and comparative intensity analysis. With consistent resolution and precision edge to edge, every frame produces more data, maximizing throughput of critical and sensitive assays. This enables quantitative molecular studies to better understand crucial mechanisms in disease and drug discovery studies. Key features of Aplo Scope Next-gen lasers Power density continuously adjustable from 0.01 through 3.325 kW/cm2 Light engine Packed with technology to enable diffraction-limited and SMLM imaging Illumination Illumination angle control from Epi to HILO and TIRF. Consistent symmetric illumination across the field-of-view Temperature control Precise temperature control up to 40°C Filter Wheel Four laser channels can be interlaced with any combination of emission filters to perform multicolor imaging, easily generating 5-color overlays Camera Sensitive next-gen CMOS camera for low noise, high-speed imaging Focus Z-lock Rapid and precise focus with locking technology to minimize Z-drift Field-Of-View 110 by 110 micrometres in both diffraction-limited and single-molecule microscopy Stage Fully automated piezo stage fits most microscopy slides and plates Mechanical stability Integral dual-stage isolation system minimizes vibration Sample enclosure Integral lid interlock ensures user safety, class 1 laser instrument Aplo Scope Aplo Scope is the flagship innovation behind the Aplo Platform: solid performance in a versatile, easy-to-use microscope. Experience a range of imaging modalities with this highly compact microscope designed to simplify multicolor super-resolution microscopy. Aplo Scope allows you to start imaging right away, no dark room or optical table is required. Its integrated software enables fixed or live imaging anywhere, and seamless up to 5 color multimode images! How much training is needed to get started using Aplo Scope? With unparalleled ease-of-use and simplicity, Aplo Scope has been designed to eliminate barriers to entry into the traditionally complex world of super-resolution microscopy and single-molecule imaging, and allow users of any skill level to utilize the instrument within one day of training. What are the dimensions of Aplo Scope? The footprint of the microscope body is as small as a standard laptop 220 mm (w) x 421 mm (d) x 242 mm (h), the light engine measures 215 mm (w) x 420 mm (d) x 456 mm (h) and looks like a computer tower. They’re attached to each other by a 1.5 m long cable and, importantly, can be used on any bench or desk without needing a dark room or optical table. Is Aplo Scope compatible with third-party kits and data analysis tools? Yes, samples can be stained with any super-resolution microscopy-compatible dye and imaged using Aplo Scope. However, this would require manual acquisition and analysis within CODI, or a third party application. ONI offers end-to-end kits where sample preparation, image acquisition, analysis and reporting are all integrated into a workflow, making imaging a seamless and more automated experience.
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