Tracking fluorescent DIVTECH for an effective cell internalization DIVERSA fluorescently-labelled lipid nanoemulsions for tracking effective cell internalization. Reference number: DIV000F1 Reagent: DIVERSA FluoGreen cell internalization Reagent Amount of reagent: 1 mL Molecules delivered: – Cell types: Adherent & suspension cells, organoids and 3D cultures Cell culture conditions: Cell culture medium supplemented with/without serum Number of experiments: 1 mL of DIVTECH Small molecule is sufficient to perform 25 delivery experiments in 6-well plates, 126 experiments in 24-well plates and 750 experiments in 96-well plates. In vivo models: Rodents and zebrafish Administration route: Intravenous, subcutaneous, intraperitoneal, intra-articular, topical… Shipping: Room Temperature Storage: -20 ºC upon arrival, once formulated at 4 ºC Provided with: DIVTECH empty vials, 2 tips for 1 mL pipette Quantitative flow cytometry analysis of in vitro cellular uptake of DIVTECH FluoGreen Uptake Kit in A549 cells in a time dependent manner (left) and to a large variety of cell lines and primary cells after two-hours incubation (right). Flow cytometry analysis of in vitro cellular delivery of DIVTECH FluoGreen Uptake Kit in H460 cells after two-hours incubation and compared to untreated cells. Confocal microscopy images showing the cell internalization of DIVTECH FluoGreen to fibroblast after two-hours incubation (blue: cell nuclei, red: cytoskeleton, green: DIVTECH FluoGreen ).
Enhancing intracellular delivery of a broad range of proteins DIVERSA lipid nanoemulsions for promoting effective intracellular and extracellular delivery of proteins. Technical information Protein MW (kDa) pl DIV031 (BR) DIV042 (Anionic) Anakinra 17.3 5.5 ✔ ✔ Green Fluorescent Protein 28.7 ~6.0 ✔ Luciferase (LucR8) 37.0 8.3 ✔ Ovalbumin 44.5 4.6 ✔ ✔ Integrin ɑ6β4 188.8 5.5 ✔ ✔ R-Phycoerythrin 250.0 5.6 ✔ ✔ β-Galactosidase 540.0 4.6 ✔ ✔ Examples of proteins efficiently associated with DIVTECH and delivered to cells Reference number DIV031 Reagent DIVERSA Protein Delivery Reagent Amount of reagent 1 mL Molecules delivered Proteins Cell types Adherent & suspension cells, organoids and 3D cultures Cell culture conditions Cell culture medium supplemented with/without serum Number of experiments 1 mL of DIVTECH Broad Range Protein kit is sufficient to perform 225 delivery experiments in 6-well plates, 1150 experiments in 24-well plates and 6815 experiments in 96-well plates. In vivo models Rodents and zebrafish Administration route Intravenous, subcutaneous, intraperitoneal, intra-articular, topical... Shipping Dry Ice Storage -20 ºC upon arrival, once formulated at 4 ºC Provided with 1 x DIVTECH empty vial, linker vial, minifilter, 2 tips for 1 mL pipette Intracellular delivery of RLuc8 protein to L3.6pl cells using DIVTECH. RLuc8 activity was measured after protein activation and detected by a luminometer. Signal was only detected (455 nm) after using DIVTECH Protein Kit, showing that the protein was efficiently delivered. Intracellular delivery of the enzyme β-Galactosidase to L3.6pl cells using DIVTECH. The β-Galactosidase activity (blue signal) was significantly enhanced when delivered by DIVTECH and showed no toxic effects as compared to commercially available competitors where cell debris and changes in the cell morphology were observed.
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Enhancing intracellular delivery of small molecules DIVERSA lipid nanoemulsions for promoting effective intracellular delivery of drugs (small molecules, APIs) Reference number: DIV010 Reagent: DIVERSA Small Molecule Delivery Reagent Amount of reagent: 4 x 1 mL Molecules delivered: Small molecules Cell types: Adherent & suspension cells, organoids and 3D cultures Cell culture conditions: Cell culture medium supplemented with/without serum Number of experiments: 1 mL of DIVTECH Small molecule is sufficient to perform 27 delivery experiments in 6-well plates, 134 experiments in 24-well plates and 800 experiments in 96-well plates. In vivo models: Rodents and zebrafish Administration route: Intravenous, subcutaneous, intraperitoneal, intra-articular, topical… Shipping: Room temperature Storage: -20 ºC upon arrival, once formulated at 4 ºC Provided with: 4x DIVTECH empty vials, 8 tips for 1 mL pipette Technical information Drug MW (g/mol) log P OW Mass (mg) % Loading (w/w) Molar (mM) % EE Galunisertib 369.42 2.4 0.75 15% 2.03 80% Doramapimod 527.66 5.7 0.75 15% 1.42 99% Disulfiram 296.54 3.9 0.75 15% 2.53 90% Paclitaxel 853.91 2.5 0.05 1% 0.06 80% Etoposide 588.56 0.6 0.05 1% 0.09 80% Doxorubicin 543.52 1.3 1 20% 1.84 90% Oleuropein 540.51 -0.4 0.75 15% 1.39 99% Rose Bengal 973.67 8.5 1 20% 1.03 95% MW: molecular weight, Log Pow: n-octanol/water partition coefficient, EE: encapsulation efficiency
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Enhancing intracellular delivery of anionic peptides DIVERSA lipid nanoeulsions for promoting effective intracellular delivery of anionic peptides. Intracellular delivery of the enzyme β-Galactosidase to L3.6pl cells using DIVTECH. The β-Galactosidase activity (blue signal) was significantly enhanced when delivered by DIVTECH and showed no toxic effects as compared to commercially available competitors where cell debris and changes in the cell morphology were observed. Reference number DIV042 Reagent DIVERSA Peptide Delivery Reagent Amount of reagent 1 mL Molecules delivered Anionic Peptides Cell types Adherent & suspension cells, organoids and 3D cultures Cell culture conditions Cell culture medium supplemented with/without serum Number of experiments 1 mL of DIVTECH Anionic Peptide Delivery Reagent is sufficient to perform 225 delivery experiments in 6-well plates, 1150 experiments in 24-well plates and 6815 experiments in 96-well plates. In vivo models Rodents and zebrafish Administration route Intravenous, subcutaneous, intraperitoneal, intra-articular, topical… Shipping Room Temperature Storage -20 ºC upon arrival, once formulated at 4 ºC Provided with 1x DIVTECH empty vial, 2 tips for 1 mL pipette
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Tracking intracellular and extracellular delivery of a broad range of proteins DIVERSA fluorescently-labelled lipid nanoemulsions for tracking and promoting effective intracellular and extracellular delivery of proteins. Reference number: DIV031F1 Reagent: DIVERSA FluoGreen Protein Delivery Reagent Amount of reagent: 1 mL Molecules delivered: Proteins Cell types: Adherent & suspension cells, organoids and 3D cultures Cell culture conditions: Cell culture medium supplemented with/without serum Number of experiments: 1 mL of DIVTECH FluoGreen Broad Range Protein Kit is sufficient to perform 25 delivery experiments in 6-well plates, 69 experiments in 24-well plates and 750 experiments in 96-well plates. In vivo models: Rodents and zebrafish Administration route: Intravenous, subcutaneous, intraperitoneal, intra-articular, topical… Shipping: Dry Ice Storage: -20 ºC upon arrival, once formulated at 4 ºC Provided with: 1 x DIVTECH empty vial, linker vial, minifilter, 2 tips for 1 mL pipette
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