How many times can qEV columns be reused? qEV columns are intended to be used no more than 5 times, with a cleaning step between each use. You can find the cleaning instructions in the respective qEV column's User Manual. Can the qEV column be regenerated? qEV columns should be cleaned/flushed between uses according to protocols in the relevant qEV user manual. If you have one of our customised large-scale columns, we can repack your column for repeated use. Are qEV columns sterile? No, qEV columns are not sterile. After production, all qEV columns are equilibrated in filtered PBS containing <0.1% ProClin 200, a common microbial control agent. For cGMP applications, GMP-ready qEV columns are manufactured using aseptic process methods and controls. Can qEV columns be used to clean EV samples after they have been incubated with fluorescent labels or antibodies? Yes. Fluorescent labels and antibodies are much smaller than EVs, so any free label or antibody can be separated from EVs. The qEV isolation platform is widely used for removing unbound labels and antibodies. What quality standards do qEV columns comply with? Izon's off-the-shelf qEV columns are designed and manufactured in Christchurch, New Zealand under a quality system certified to ISO 13485:2016. How many qEV columns come in a pack? - qEVsingle: 20 Columns - qEVoriginal: 5 or 100 Columns - qEV1: 5 Columns - qEV2: 2 Columns - qEV10: Sold individually - qEV100: Sold individually What is the difference between the 20 nm, 35 nm and 70 nm columns? qEV columns come in three series – 20 nm, 35 nm and 70 nm – named after the pore size of the resin used. Each series offers a different balance of particle recovery and purity: the 20 nm series maximises recovery, the 35 nm series balances recovery and purity, and the 70 nm series delivers the purest EV isolates with the highest level of lipoprotein depletion. This trade-off comes down to size exclusion chromatography: larger particles like EVs pass around the resin beads and elute first, while proteins and other small molecules detour through the pores and trail behind. Larger pores trap more of these small molecules (leading to purer isolates); smaller pores trap fewer (leading to higher recovery, including smaller EVs). For more information, visit the qEV series comparison page or get in touch with the team for more guidance. How do I determine which column I should use for my application? Choose your qEV column size based on your sample loading volume. To choose your column type, you will need to consider whether you would prefer to prioritise particle recovery or particle purity. Visit the qEV series comparison page to compare qEV series. If you are still unsure, reach out to our friendly support staff for guidance. Is it possible to tailor fractions so that they correspond to a specific size profile? No. The column length is not long enough to achieve this type of resolution effectively. Is it possible that the protein fractions will contain EVs? Yes. Depending on the size of the resin pores, particles that are smaller than the pores will enter the resin and their progress through the column will be slowed. Hence, these particles may appear in later fractions. Columns have been optimised to reduce this as much as possible. What buffer should be used to store the column between uses? Load the column with PBS containing a bactericide or bacteriostatic agent (e.g., 0.05% w/v sodium azide or 0.05% ProClin 200), or 20% ethanol. Column cleaning and storage instructions are provided in qEV User Manuals. Can I load a plasma sample directly onto a qEV column? Most 'raw' samples cannot be directly run on qEV columns without prior preparation steps to remove large particulate matter. qEV user manuals provide recommendations on how to prepare your sample appropriately. Are there any discounts available when buying in bulk? Yes — we don't want price to be a barrier to scale-up. qEVoriginal columns are available in bulk packs of 100 for a discounted price, and volume discounts can be arranged across all column types for large orders. Get in touch with our friendly sales team for more information on bulk pricing. Which qEV columns can be used with the Automatic Fraction Collector (AFC)? The following qEV columns can be used with the AFC: qEVsingle, qEVoriginal, qEV1, qEV2 and qEV10. The qEV100 and larger customised qEV columns are not AFC-compatible. How do I know which fractions will contain my target particles? Eluted volumes containing your particles of interest will vary in composition based on the sample, sample preparation and column type. You will need to study the protein and EV content of your fractions and identify which portion is optimal to collect. This will vary depending on whether you would prefer to maximise isolate purity or particle recovery. Typical EV elution profiles for human plasma samples can be found in qEV User Manuals and may serve as a useful starting point. Why does column size and sample loading volume matter? Selecting the optimal column size for your sample volume is important for maximising the purity of EVs in your sample. Loading a sample volume higher than what is recommended results in a lower level of purity in later vesicle-containing volumes, a greater overlap between protein and EV elution peaks, and a higher protein peak within the purified containing volume. Loading a sample volume lower than what is recommended results in a higher dilution factor of the sample. Check the optimal recommended sample volume for your qEV column. What's the difference between qEV Gen 2 and competitor columns? Gen 2 qEV columns are built with a novel, high-performance agarose resin which provides a more purified EV-containing sample, compared to the resin previously used in our earlier (now discontinued) Legacy columns. This legacy-style resin is still used by some other providers in the market today, meaning Izon's Gen 2 resin offers a level of purification performance that has moved beyond the industry standard. What are GMP-ready qEV columns? GMP-ready qEV columns are manufactured and quality-controlled to support use in GMP-regulated environments. Although qEV users are still responsible for regulatory compliance and related risk assessments, the additional measures put in place for GMP-ready column production provide a good starting point if you are working in GMP environments. GMP-ready qEV columns are filled using aseptic methods, with final sanitisation steps performed in a laminar flow cabinet that provides a local environment meeting the ISO Class 7 (Class 10,000) clean room Non-Viable Particulate (NVP) standard. Each batch also undergoes additional cleaning steps and rigorous quality control testing for bioburden and endotoxin levels, against strict targets that must be met before release. Which qEV columns are compatible with the qEV DXter? Up to 24 qEVsingle, qEVoriginal or qEV1 columns can be processed by the qEV DXter, our robotic automated liquid handling instrument for parallel processing. To learn more visit the qEV DXter webpage. Which qEV columns are compatible with the qEV Zenco? The qEV2, qEV10, qEV100 and larger customised columns can be processed by the qEV Zenco, our automated chromatography system for purifying samples. To learn more visit the Zenco webpage.
Using Tunable Resistive Pulse Sensing (TRPS), the Exoid measures nanoparticles one-by-one as they pass through a tunable nanopore, allowing you to obtain measurements of particle size and concentration, or particle size and zeta potential, on a single-particle basis. Unlike ensemble techniques, where larger particles can skew size measurements, the Exoid provides insightful, high-resolution measurements that allow you to detect important differences in particle size distribution. The Exoid is capable of measuring polydisperse samples over a wide size distribution, at a high resolution. This allows you to identify subpopulations within your sample and obtain an accurate measure of the overall concentration and number-based particle size distribution. Tunable Resistive Pulse Sensing (TRPS) measurements have in-built requirements which ensure a standardised approach to measurement: Traceable calibration - Measurements are calibrated using Izon standards characterised against NIST particles, ensuring reproducible and comparable results. Real-time system feedback - Live monitoring of current and noise helps maintain stable, high-quality measurements. Buffer control checks - Running a particle-free buffer confirms that detected events represent true particles in the sample. EV-ready workflow - Pair with qEV isolation to support consistent, standardised EV characterisation. The Exoid enables detailed, single-particle analysis across a broad range of nanoparticle types. Measure particle size and concentration from 40 nm to 11 µm, and size and zeta potential from 40 nm to 2,000 nm, using interchangeable nanopores to match your sample. Its wide dynamic range and tunable measurement conditions make it well suited to complex or heterogeneous samples, including extracellular vesicles, lipid nanoparticles, virus-like particles, and other biological or synthetic nanoparticles. For researchers who require flexibility and depth of insight across diverse particle systems, the Exoid remains a trusted and versatile platform. Over the years, TRPS instruments have evolved significantly, improving usability and workflow compared with earlier generations. That said, the Exoid remains a hands-on platform. Users adjust measurement conditions and occasionally troubleshoot to maintain optimal performance, particularly when working with complex samples. With training and experience, this level of control becomes a strength, enabling detailed analysis across a wide range of particle types. Izon provides training and ongoing support to help users get the most from the system. As particle size decreases, measurement becomes more technically demanding. Smaller particles require tighter control of conditions, careful calibration, and greater user skill to obtain reliable data. The Exoid is designed to deliver precise, repeatable single-particle measurements across a wide size range, including challenging sub-100 nm particles. Achieving optimal results requires good sample preparation and active user oversight. For experienced users who value flexibility and control, this involvement enables robust, defensible data, particularly at the smallest sizes. Specifications of the Exoid TRPS Measurement System Analysis range: 40 nm to 11 µm Compatible electrolyte solutions: Include PBS, HEPES, MES and KCI Footprint: 300 x 300 mm Concentration range: 1E5 to 1E11 particles/mL (size-dependent) Weight: 10.8 kg Height: 250 mm Purchase price $47.5K Lease $4.5K / month
CATEGORY
A New Era in Nanoparticle Characterisation High-Resolution Nanoparticle Measurement, Made Simple The Pulsoid is Izon’s brand-new nanoparticle measurement system, delivering high-resolution, single-particle insight through a faster, more intuitive workflow. Powered by Nanopore Pulse Sensing (NPS), the Pulsoid removes the traditional trade-off between measurement performance and usability, making direct measurement of particle size, concentration, and zeta potential more accessible. See What Ensemble Methods Miss Ensemble techniques report averages - but important differences between particles can be hidden beneath them. The Pulsoid provides particle-by-particle insights, allowing you to explore distributions, heterogeneity, and subtle population differences that may otherwise go undetected. Exceptionally Accurate Size Measurement Access high-resolution measurements of size and concentration, with TEM-level size accuracy¹. The precision and consistency of solid-state nanopores enable accurate measurement of small particles from 40 nm and above. Size accuracy is exceptionally high, demonstrated by 99.7% agreement with TEM for NIST-traceable polystyrene standards. Single-particle analysis ensures the Pulsoid maintains accuracy even when analysing polydisperse samples. High-Throughput, Easy to Learn A solid-state silicon nanopore housed in an easy-to-clean fluid cell reduces handling, maintenance, and calibration between runs. The result is faster workflows and higher throughput. With a simplified setup and fewer opportunities for user error, the Pulsoid requires only a fraction of the setup time of the Exoid and delivers around double 2 the throughput. It also offers a significantly lower learning curve and more consistent results across users. True Particle-Count–Based Concentration Measurement The Pulsoid measures concentration by directly counting individual particles, delivering a linear response across a wide concentration range of 10⁹ to 10¹² particles per mL. This ensures measured changes reflect real differences in particle number, not artefacts, making the Pulsoid well suited to formulation work, process monitoring, and comparability studies. Direct, Single-Particle Zeta Potential The Pulsoid measures particle size and zeta potential simultaneously on a particle-by-particle basis, revealing surface charge detail that ensemble techniques average away. Resolving charge distributions and subpopulations provides clearer insight into formulation behaviour, stability, and process-related changes, particularly for heterogeneous samples. A New Solid-State Nanopore Chip at the Core At the heart of the Pulsoid is a precision-manufactured silicon nanopore chip. Its fixed pore geometry delivers consistent, repeatable single-particle measurements with the resolution required for high-quality nanoparticle analysis. As particles pass through the nanopore, brief changes in ionic current are measured.Signal magnitude correlates with particle size, event frequency reflects concentration, and event duration enables zeta potential measurement. The result is highly detailed, particle by particle characterisation. Optimised for LNPs and Beyond The Pulsoid is designed for lipid nanoparticles and other nanoscale particle types used in biological and therapeutic research. High-resolution, single-particle analysis helps detect subtle changes caused by aggregation, instability, storage conditions, or processing effects. With access to size, concentration, and zeta potential data, you can build a clearer picture of particle behaviour and stability over time.
CATEGORY
Measure Nanoparticles with Single Particle Resolution Using Tunable Resistive Pulse Sensing (TRPS), the Exoid measures nanoparticles one-by-one as they pass through a tunable nanopore, allowing you to obtain measurements of particle size and concentration, or particle size and zeta potential, on a single-particle basis. Unlike ensemble techniques, where larger particles can skew size measurements, the Exoid provides insightful, high-resolution measurements that allow you to detect important differences in particle size distribution. The Exoid is capable of measuring polydisperse samples over a wide size distribution, at a high resolution. This allows you to identify subpopulations within your sample and obtain an accurate measure of the overall concentration and number-based particle size distribution. A Proven, Standardised Platform Tunable Resistive Pulse Sensing (TRPS) measurements have in-built requirements which ensure a standardised approach to measurement: - Traceable calibration: Measurements are calibrated using Izon standards characterised against NIST particles, ensuring reproducible and comparable results. - Real-time system feedback: Live monitoring of current and noise helps maintain stable, high-quality measurements. - Buffer control checks: Running a particle-free buffer confirms that detected events represent true particles in the sample. - EV-ready workflow: Pair with qEV isolation to support consistent, standardised EV characterisation. Characterise Your Samples Confidently With the Exoid The Exoid enables detailed, single-particle analysis across a broad range of nanoparticle types. Measure particle size and concentration from 40 nm to 11 µm, and size and zeta potential from 40 nm to 2,000 nm, using interchangeable nanopores to match your sample. Its wide dynamic range and tunable measurement conditions make it well suited to complex or heterogeneous samples, including extracellular vesicles, lipid nanoparticles, virus-like particles, and other biological or synthetic nanoparticles. For researchers who require flexibility and depth of insight across diverse particle systems, the Exoid remains a trusted and versatile platform. What Can You Expect From the Exoid? Over the years, TRPS instruments have evolved significantly, improving usability and workflow compared with earlier generations. That said, the Exoid remains a hands-on platform. Users adjust measurement conditions and occasionally troubleshoot to maintain optimal performance, particularly when working with complex samples. With training and experience, this level of control becomes a strength, enabling detailed analysis across a wide range of particle types. Izon provides training and ongoing support to help users get the most from the system. Skill Required for Insightful, Reliable Nanoparticle Measurements As particle size decreases, measurement becomes more technically demanding. Smaller particles require tighter control of conditions, careful calibration, and greater user skill to obtain reliable data. The Exoid is designed to deliver precise, repeatable single-particle measurements across a wide size range, including challenging sub-100 nm particles. Achieving optimal results requires good sample preparation and active user oversight. For experienced users who value flexibility and control, this involvement enables robust, defensible data, particularly at the smallest sizes. Specifications of the Exoid TRPS Measurement System Analysis range: 40 nm to 11 µm Compatible electrolyte solutions: Include PBS, HEPES, MES and KCI Footprint: 300 x 300 mm Concentration range: 1E5 to 1E11 particles/mL (size-dependent) Weight: 10.8 kg Height: 250 mm There are a number of ways you can incorporate the accuracy and precision of TRPS in your research. You can buy the Exoid outright for $47,500 USD, or get in touch with our team to discuss a lease agreement. Alternatively, we also offer sample measurement services for our EU and US customers. All prices listed below are in USD. Purchase price $47.5K Lease $4.5K / month
CATEGORY
Tunable Resistive Pulse Sensing (TRPS) is a true single-particle technique which uses changes in electrical current to evaluate individual particles suspended in an electrolyte solution as they move between electrodes through a tunable nanopore. Current flow is monitored, and each particle is detected as it passes through the nanopore, due to the transient change in current that is subsequently created. Physical characteristics of particles in the sample are obtained via the analysis of resulting blockades, which represent individual particles. Specifically, particle volume is directly proportional to blockade magnitude, and particle concentration is precisely calculated from the particle flow rate measured at several different applied pressures. Zeta potential is derived via measurements of electrophoretic mobility, which is calculated from the velocity with which the particle traverses the nanopore. Tunable Resistive Pulse Sensing (TRPS) is a single-particle measurement technique that generates high-resolution data on the physical characteristics of nanoparticles. Given the direct relationships that exist between blockade characteristics and physical properties of nanoparticles, TRPS enables true particle-by-particle analysis. The resulting high-resolution measurements enable you to gain deep insights on particle characteristics. Different sized nanopores are available to facilitate a range of particle sizes, from 40 nm to 11 µm in diameter, and only 35 μL of sample is needed for a TRPS measurement. With TRPS, the use of standardised calibration particles and ability to monitor the signal-to-noise ratio in real time ensures that conditions are optimised and maintained throughout the calibration and measurement process. Key features of tunable resistive pulse sensing (TRPS) showing nanoparticles passing through a nanopore (top left), signal trace with resistive pulses (top right), high-resolution particle size distribution (bottom left), and a breakdown of how each individual blockade is analysed (bottom right). Approach to size measurement: Particle size is directly proportional to blockade magnitude; each particle is measured individually. Size range capabilities: Wide size range; particles from 40 nm - 11 µm can be measured using appropriately sized nanopores. Limitations of size analysis: Different sized nanopores are needed to cover the entire size range. Resolution capabilities: Can resolve subpopulations in multimodal samples. Concentration: Calculated directly from blockade frequency, using standardised calibration particles of known size and concentration. Ease of data processing: Automated data processing with user-friendly data visualisation interface. Influence of instrument parameters/particle properties: Parameters are actively monitored to ensure they are optimised for the measurement of calibration and sample particles.
CATEGORY
