By BioNavis Ltd
Characterization and interaction measurements of EVs are crucial parts of the research process. Extracellular vesicle size and concentration MP-SPR is an ideal tool for measuring EV size, especially for EVs smaller than 100nm due to high sensitivity of the technology. The unique multi-wavelength setup of MP-SPR, along with complete SPR curve measurements, are crucial features for size and concentration measurements. Extracellular Vesicle corona BioNavis MP-SPR measurements can be performed with complex media such as cell culture media, plasma or serum. Plasma proteins have been shown to interact with EVs, and MP-SPR has been used to study the interaction between vesicles and serum proteins forming protein corona. As the measurements are performed on dynamic flow, this allows the study of proteins forming hard and soft corona on EVs, samples can be obtained for further analysis. Affinity and kinetics MP-SPR measures the binding affinity of EVs with high precision and their diagnostic molecules (such as antibodies, peptides, or aptamers) without labels. The dynamic flow principle also provides binding kinetics. The derived kinetic measurements can be used to improve the diagnostic molecule interaction parameters. Cellular uptake MP-SPR allows you to either form a lipid bilayer on the SiO2 sensor surface to mimic the cellular interaction or culture adherent cells on suitable sensor surface. MP-SPR’s flexible sensor slide design allows the use of standard cell culture protocols to form a confluent cell layer on a sensor slide ex situ. Dynamic measurements can be used to observe the uptake of extracellular vesicles in living cells. This unique capability, enabled by the complete SPR curve measurement of MP-SPR, provides new insights into EV uptake. Additionally, after real-time MP-SPR measurements, the sensor slide can be further validated ex situ with microscopy techniques, including AFM and SEM.
