We are expert in large-scale quantitative proteomics, structural mass spectrometry, and bioinformatics for the in depth study of proteomes and signaling pathways, and for the characterization of pivotal proteins and protein complexes, like the proteasome. We developed integrated strategies using in-vivo crosslinking, affinity-purification and quantitative proteomics to characterize the diversity of proteasome complexes and their dynamics in various cells, physiological and pathological contexts. We then optimized structural MS approaches to determine the structural features that govern protein-protein interactions and allosteric changes during proteasome association to its regulatory protein complexes.
