By NanoBrand
Dynamic light scattering (DLS), zeta potential, and UV-vis spectroscopy provide complementary data on nanoparticle size, surface properties, and dispersion stability. DLS measures hydrodynamic diameter, including the particle and its surface layer in liquid, which is typically larger than the TEM diameter. NanoBrand measures hydrodynamic diameter and zeta potential, which characterizes effective particle-surface charge, using a Malvern ZSU5600. UV-visible spectra are recorded on a PerkinElmer Lambda 365+ spectrophotometer from 200 to 1100 nm in 1 nm steps. For gold and silver nanoparticles, changes in the plasmon peak, including shifts or broadening, can indicate aggregation or surface change. See our UV-vis stability testing guide for practical interpretation and monitoring.