By Queen Mary University of London
State-of-the art, fast scanning, near super-resolution confocal microscope. The Airyscan detector makes it possible to increase the resolution of your imaging far beyond the resolution of a classic confocal point scanning microscope. In addition, images can be acquired with high sensitivity and high speed. Fast scanning: 27 frames/sec @ 480 x 480 pixels. Resolution: max 140nm lateral and 400nm axial resolution. Light source: The system is equipped with a Diode 405nm laser, a Multiline Argon laser (wavelengths 458nm, 488nm and 514nm), a DPSS 561nm laser, a HeNe 594nm laser and a HeNe 633nm laser. Additionally, a transmitted light detector is available. Detectors: The confocal has a range of ultra-sensitive detectors (2x PMT, 1x GaAsP, 1x Airyscan). Available objectives: - Plan-Apochromat, 10x (NA: 0.45; WD:2.0), Dry - Plan-Apochromat, 20x (NA: 0.80; WD:0.55), Dry - Plan-Apochromat, 40x (NA: 1.3; DIC UVVIS-IR), Oil - Plan-Apochromat, 63x (NA: 1.4; DIC f/ELYRA), Oil Software: - ZEN Black software interface - Modules for 3D/4D reconstruction and animation; spectral scanning; analysis and quantification of co-localisation; spectral unmixing, advanced tiling and photo-bleaching (FRAP, FRET). Other features: - An incubation system with temperature and CO2 control. - Fully motorised stage - Piezo z-drive for accurate high-speed z-stacks Costs Price per hour:£25 Contact details Facility:Endocrinology Campus:WHRI (1st floor) CHS Address:Endocrinology, John Vane Building, Charterhouse Square, London, EC1M 6BQ Manager:Lisa Romano & Paul Chapple Contact:020 7882 6242
