Automation of the SUPR-DSF, whether modular or fully integrated allows increased capacities, more sample or condition screening and collection of data for the user to analyse. To illustrate the accuracy, and repeatability of microplate-based screening with the SUPR-DSF, 16 x 384-well microplates were prepared with lysozyme using a modular approach with each stage of the process running independently. Full integration of the modular workstations is easily achieved should additional automation and walk-away time be desired. Thermal denaturation data from over 6000 replicates was collected and the statistics, including mean and standard deviations presented. The whole process achieved a standard deviation of only 0.14°C, across all 16 microplates while reducing the amount of sample consumed. This not only lends confidence to the use of microplates, in the screening of proteins' conformational stability, but also shows how the modern platform of the SUPR-DSF can be connected in a wider screening protocol.
